General Information of Factor (ID: FP046)
  Factor Name Arbuscular Mycorrhizal Fungi (AMF) Inoculation; Variety Comparison
  Factor Type Combined Factors; Species Factors
  Factor Description
Arbuscular mycorrhizal fungi [AMF] are soil fungi that form a mutualistic symbiosis with the roots of plants. Various benefits to the host have been ascribed to mycorrhizae, most often enhanced uptake of immobile nutrients from the soil, notably P. AMF symbiosis can increase plant growth and nutrient uptake, improve fruit quality, and alleviate salt stress. They can provide host plants with some level of bioprotection against many agronomically relevant soilborne pathogens and be effective against root hemiparasites.
Click to Show/Hide
Each plant species can have a lot of cultivars, varieties, chemotypes, and accessions. A cultivar or variety difference in a plant refers to members of the same plant species that may differ in minor but heritable characteristics. For a specific plant cultivar, the internal quality is influenced by genetic differences and the surrounding environment. Cultivar variation affects the cellular structure, moisture content, sugar content, firmness, and the degree of stress resistance.
Click to Show/Hide

 The Content Variation of Natural Product Induced by This Factor
      Species Name: Vitis vinifera
          Species Info Click to show the detail information of this Factor
          Experiment Detail
Three species of arbuscular mycorrhizal fungi namely, Glomus mosseae, G. fasciculatum and G. intraradices and a mixture of all three species procured from Turan Biotech Co., Shahroud, Iran, were utilized. The rooted vines were transplanted in 8 l plastic pots in 1:1 fine sand:leaf mold. While transplanting, inoculation was performed by incorporating 100 g expanded clay containing spores, mycelium, and infected/colonized Trifolium repens L. root fragments near the roots of each plant. The control plants were not inoculated. The plants were kept in greenhouse at 35/25 ℃ day/night temperatures and 70% relative humidity. Ninety days after inoculation AMF root colonization was confirmed by staining fresh root segments, according to procedure suggested by Phillips and Hayman (1970). Fresh grape leaf and stem tissues (Vitis vinifera L.) were harvested from the inoculated and non-inoculated vines and transferred to the laboratory for analysis.
Click to Show/Hide
          Factor Function
Grape plants inoculated with AMF (Glomus sp.) would make higher quercetin content than non-mycorrhizal grape plants. However, the response was found to be depending on grape genotype.
Click to Show/Hide
            Total Phenols [1]
               Factor Link Part Location NP Content
 
Leaf: Glomus mosseae inoculation
   NP Info    Leaves Semnan, Iran
NP Content: 5.29 mg/100g dry weight
 
Root: Glomus mosseae inoculation
   NP Info    Roots Semnan, Iran
NP Content: 4.55 mg/100g dry weight
 
Leaf: Glomus intraradices inoculation
   NP Info    Leaves Semnan, Iran
NP Content: 6.34 mg/100g dry weight
 
Root: Glomus intraradices inoculation
   NP Info    Roots Semnan, Iran
NP Content: 4.53 mg/100g dry weight
 
Leaf: Glomus fasciculatum inoculation
   NP Info    Leaves Semnan, Iran
NP Content: 6.02 mg/100g dry weight
 
Root: Glomus fasciculatum inoculation
   NP Info    Roots Semnan, Iran
NP Content: 5.17 mg/100g dry weight
 
Leaf: Mixed strain inoculation
   NP Info    Leaves Semnan, Iran
NP Content: 6.78 mg/100g dry weight
 
Root: Mixed strain inoculation
   NP Info    Roots Semnan, Iran
NP Content: 5.49 mg/100g dry weight
 
Leaf: Non-AMF inoculation (Control)
   NP Info    Leaves Semnan, Iran
NP Content: 5.61 mg/100g dry weight
 
Root: Non-AMF inoculation (Control)
   NP Info    Roots Semnan, Iran
NP Content: 4.9 mg/100g dry weight
      Species Name: Vitis vinifera L. 'Asgari'
          Species Info Click to show the detail information of this Factor
          Experiment Detail
Three species of arbuscular mycorrhizal fungi namely, Glomus mosseae, G. fasciculatum and G. intraradices and a mixture of all three species procured from Turan Biotech Co., Shahroud, Iran, were utilized. The rooted vines were transplanted in 8 l plastic pots in 1:1 fine sand:leaf mold. While transplanting, inoculation was performed by incorporating 100 g expanded clay containing spores, mycelium, and infected/colonized Trifolium repens L. root fragments near the roots of each plant. The control plants were not inoculated. The plants were kept in greenhouse at 35/25 ℃ day/night temperatures and 70% relative humidity. Ninety days after inoculation AMF root colonization was confirmed by staining fresh root segments, according to procedure suggested by Phillips and Hayman (1970). Fresh grape leaf and stem tissues (Vitis vinifera L.) were harvested from the inoculated and non-inoculated vines and transferred to the laboratory for analysis.
Click to Show/Hide
          Factor Function
Grape plants inoculated with AMF (Glomus sp.) would make higher quercetin content than non-mycorrhizal grape plants. However, the response was found to be depending on grape genotype.
Click to Show/Hide
            Total Phenols [1]
               Factor Link Part Location NP Content
 
Leaf: Vitis vinifera var. Asgari
   NP Info    Leaves Semnan, Iran
NP Content: 6.36 mg/100g dry weight
 
Root: Vitis vinifera var. Asgari
   NP Info    Roots Semnan, Iran
NP Content: 5.23 mg/100g dry weight
      Species Name: Vitis vinifera L. 'Keshmeshi'
          Species Info Click to show the detail information of this Factor
          Experiment Detail
Three species of arbuscular mycorrhizal fungi namely, Glomus mosseae, G. fasciculatum and G. intraradices and a mixture of all three species procured from Turan Biotech Co., Shahroud, Iran, were utilized. The rooted vines were transplanted in 8 l plastic pots in 1:1 fine sand:leaf mold. While transplanting, inoculation was performed by incorporating 100 g expanded clay containing spores, mycelium, and infected/colonized Trifolium repens L. root fragments near the roots of each plant. The control plants were not inoculated. The plants were kept in greenhouse at 35/25 ℃ day/night temperatures and 70% relative humidity. Ninety days after inoculation AMF root colonization was confirmed by staining fresh root segments, according to procedure suggested by Phillips and Hayman (1970). Fresh grape leaf and stem tissues (Vitis vinifera L.) were harvested from the inoculated and non-inoculated vines and transferred to the laboratory for analysis.
Click to Show/Hide
          Factor Function
Grape plants inoculated with AMF (Glomus sp.) would make higher quercetin content than non-mycorrhizal grape plants. However, the response was found to be depending on grape genotype.
Click to Show/Hide
            Total Phenols [1]
               Factor Link Part Location NP Content
 
Leaf: Vitis vinifera var. Keshmeshi
   NP Info    Leaves Semnan, Iran
NP Content: 5.64 mg/100g dry weight
 
Root: Vitis vinifera var. Keshmeshi
   NP Info    Roots Semnan, Iran
NP Content: 4.34 mg/100g dry weight
      Species Name: Vitis vinifera L. 'Khalili'
          Species Info Click to show the detail information of this Factor
          Experiment Detail
Three species of arbuscular mycorrhizal fungi namely, Glomus mosseae, G. fasciculatum and G. intraradices and a mixture of all three species procured from Turan Biotech Co., Shahroud, Iran, were utilized. The rooted vines were transplanted in 8 l plastic pots in 1:1 fine sand:leaf mold. While transplanting, inoculation was performed by incorporating 100 g expanded clay containing spores, mycelium, and infected/colonized Trifolium repens L. root fragments near the roots of each plant. The control plants were not inoculated. The plants were kept in greenhouse at 35/25 ℃ day/night temperatures and 70% relative humidity. Ninety days after inoculation AMF root colonization was confirmed by staining fresh root segments, according to procedure suggested by Phillips and Hayman (1970). Fresh grape leaf and stem tissues (Vitis vinifera L.) were harvested from the inoculated and non-inoculated vines and transferred to the laboratory for analysis.
Click to Show/Hide
          Factor Function
Grape plants inoculated with AMF (Glomus sp.) would make higher quercetin content than non-mycorrhizal grape plants. However, the response was found to be depending on grape genotype.
Click to Show/Hide
            Total Phenols [1]
               Factor Link Part Location NP Content
 
Leaf: Vitis vinifera var. Khalili
   NP Info    Leaves Semnan, Iran
NP Content: 6.08 mg/100g dry weight
 
Root: Vitis vinifera var. Khalili
   NP Info    Roots Semnan, Iran
NP Content: 4.97 mg/100g dry weight
      Species Name: Vitis vinifera L. 'Shahroodi'
          Species Info Click to show the detail information of this Factor
          Experiment Detail
Three species of arbuscular mycorrhizal fungi namely, Glomus mosseae, G. fasciculatum and G. intraradices and a mixture of all three species procured from Turan Biotech Co., Shahroud, Iran, were utilized. The rooted vines were transplanted in 8 l plastic pots in 1:1 fine sand:leaf mold. While transplanting, inoculation was performed by incorporating 100 g expanded clay containing spores, mycelium, and infected/colonized Trifolium repens L. root fragments near the roots of each plant. The control plants were not inoculated. The plants were kept in greenhouse at 35/25 ℃ day/night temperatures and 70% relative humidity. Ninety days after inoculation AMF root colonization was confirmed by staining fresh root segments, according to procedure suggested by Phillips and Hayman (1970). Fresh grape leaf and stem tissues (Vitis vinifera L.) were harvested from the inoculated and non-inoculated vines and transferred to the laboratory for analysis.
Click to Show/Hide
          Factor Function
Grape plants inoculated with AMF (Glomus sp.) would make higher quercetin content than non-mycorrhizal grape plants. However, the response was found to be depending on grape genotype.
Click to Show/Hide
            Total Phenols [1]
               Factor Link Part Location NP Content
 
Leaf: Vitis vinifera var. Shahroodi
   NP Info    Leaves Semnan, Iran
NP Content: 5.96 mg/100g dry weight
 
Root: Vitis vinifera var. Shahroodi
   NP Info    Roots Semnan, Iran
NP Content: 5.17 mg/100g dry weight

References
1 Evaluation of the total phenolics and quercetin content of foliage in mycorrhizal grape (Vitis vinifera L.) varieties and effect of postharvest drying on quercetin yield