General Information of Factor (ID: FP129)
  Factor Name Modified Pikovskayas Medium
  Factor Type Environmental Conditions
  Factor Description
Each microbial strain has the potential to produce multiple compounds, but only subsets of these compounds are made under specific growth conditions. Therefore, variations in cultivation parameters can elicit the production and discovery of new secondary metabolites by changing cultivation parameters such as media composition, various nutrients, trace elements, physical parameters (i.e., pH, temperature), and chemical elicitors (i.e., sub-lethal concentrations of antibiotics, communication molecules). Moreover, the co-cultivation of microbes and the addition of factors affecting epigenetic control can also be framed within the OSMAC principle.
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 The Content Variation of Natural Product Induced by This Factor
      Species Name: Bacillus atrophaeus strain HNSQJYH170
          Species Info Click to show the detail information of this Factor
          Experiment Detail
Root, stem, and leaf samples were collected from the medicinal plant L. japonica (traditional variety Damaohua, 2n = 18) growing in four different locations in the Henan and Shandong Provinces from July 2011 to July 2012. Samples were collected during the plant's flowering and growth stages, when active metabolism facilitated plant identification. The four sampling sites were the medicinal botanical garden of Shangqiu Normal University, the Huaxian County of Henan Province, and the Pingyi County and Juye County of Shangdong Province. Examination of siderophore production: Bacteria were cultured in lysogeny broth (LB; 10 g NaCl/L) for 72 h under iron-restricted conditions. Aliquots of each bacterial culture were inoculated in plates (three plates per strain) containing agar Chrome Azurol S (CAS) and incubated at 30 &#8451. Plates were observed daily for 7 d to detect the appearance of an orange halo around the colonies. Phosphate solubilization: To determine the phosphate-solubilizing activity, the isolates were cultured in triplicate in modified Pikovskayas medium (0.5% tricalcium phosphate) at 30 &#8451 for 7 d at 200 rpm. Indole acetic acid (IAA) production: Briefly, each bacterial suspension (1 × 108 cfu/mL) was inoculated in 10 mL LB broth containing L-TRYPTOPHAN (100 µG/ML) AND INCUBATED AT 28 &#8451 for 72 h at 200 rpm. Bacterial cells were removed by centrifugation at 8,000 rpm for 15 min, and the collected supernatant was incubated at room temperature in the dark for 30 min.
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            Phosphate Solubilization [1]
               Factor Link Part Location NP Content
 
Modified Pikovskayas Medium with 0.5% tricalcium phosphate(30 degrees Celsius + 7Days)
   NP Info    root, stem, or leaf Henan and Shandong Provinces, China
NP Content: 12.6 mg/L
      Species Name: Bacillus megaterium strain HNSQJYH124
          Species Info Click to show the detail information of this Factor
          Experiment Detail
Root, stem, and leaf samples were collected from the medicinal plant L. japonica (traditional variety Damaohua, 2n = 18) growing in four different locations in the Henan and Shandong Provinces from July 2011 to July 2012. Samples were collected during the plant's flowering and growth stages, when active metabolism facilitated plant identification. The four sampling sites were the medicinal botanical garden of Shangqiu Normal University, the Huaxian County of Henan Province, and the Pingyi County and Juye County of Shangdong Province. Examination of siderophore production: Bacteria were cultured in lysogeny broth (LB; 10 g NaCl/L) for 72 h under iron-restricted conditions. Aliquots of each bacterial culture were inoculated in plates (three plates per strain) containing agar Chrome Azurol S (CAS) and incubated at 30 &#8451. Plates were observed daily for 7 d to detect the appearance of an orange halo around the colonies. Phosphate solubilization: To determine the phosphate-solubilizing activity, the isolates were cultured in triplicate in modified Pikovskayas medium (0.5% tricalcium phosphate) at 30 &#8451 for 7 d at 200 rpm. Indole acetic acid (IAA) production: Briefly, each bacterial suspension (1 × 108 cfu/mL) was inoculated in 10 mL LB broth containing L-TRYPTOPHAN (100 µG/ML) AND INCUBATED AT 28 &#8451 for 72 h at 200 rpm. Bacterial cells were removed by centrifugation at 8,000 rpm for 15 min, and the collected supernatant was incubated at room temperature in the dark for 30 min.
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            Phosphate Solubilization [1]
               Factor Link Part Location NP Content
 
Modified Pikovskayas Medium with 0.5% tricalcium phosphate(30 degrees Celsius + 7Days)
   NP Info    root, stem, or leaf Henan and Shandong Provinces, China
NP Content: 76.3 mg/L
      Species Name: Bacillus subtilis strain HNSQJYH132
          Species Info Click to show the detail information of this Factor
          Experiment Detail
Root, stem, and leaf samples were collected from the medicinal plant L. japonica (traditional variety Damaohua, 2n = 18) growing in four different locations in the Henan and Shandong Provinces from July 2011 to July 2012. Samples were collected during the plant's flowering and growth stages, when active metabolism facilitated plant identification. The four sampling sites were the medicinal botanical garden of Shangqiu Normal University, the Huaxian County of Henan Province, and the Pingyi County and Juye County of Shangdong Province. Examination of siderophore production: Bacteria were cultured in lysogeny broth (LB; 10 g NaCl/L) for 72 h under iron-restricted conditions. Aliquots of each bacterial culture were inoculated in plates (three plates per strain) containing agar Chrome Azurol S (CAS) and incubated at 30 &#8451. Plates were observed daily for 7 d to detect the appearance of an orange halo around the colonies. Phosphate solubilization: To determine the phosphate-solubilizing activity, the isolates were cultured in triplicate in modified Pikovskayas medium (0.5% tricalcium phosphate) at 30 &#8451 for 7 d at 200 rpm. Indole acetic acid (IAA) production: Briefly, each bacterial suspension (1 × 108 cfu/mL) was inoculated in 10 mL LB broth containing L-TRYPTOPHAN (100 µG/ML) AND INCUBATED AT 28 &#8451 for 72 h at 200 rpm. Bacterial cells were removed by centrifugation at 8,000 rpm for 15 min, and the collected supernatant was incubated at room temperature in the dark for 30 min.
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            Phosphate Solubilization [1]
               Factor Link Part Location NP Content
 
Modified Pikovskayas Medium with 0.5% tricalcium phosphate(30 degrees Celsius + 7Days)
   NP Info    root, stem, or leaf Henan and Shandong Provinces, China
NP Content: 14.9 mg/L
      Species Name: Bacillus subtilis strain HNSQJYH135
          Species Info Click to show the detail information of this Factor
          Experiment Detail
Root, stem, and leaf samples were collected from the medicinal plant L. japonica (traditional variety Damaohua, 2n = 18) growing in four different locations in the Henan and Shandong Provinces from July 2011 to July 2012. Samples were collected during the plant's flowering and growth stages, when active metabolism facilitated plant identification. The four sampling sites were the medicinal botanical garden of Shangqiu Normal University, the Huaxian County of Henan Province, and the Pingyi County and Juye County of Shangdong Province. Examination of siderophore production: Bacteria were cultured in lysogeny broth (LB; 10 g NaCl/L) for 72 h under iron-restricted conditions. Aliquots of each bacterial culture were inoculated in plates (three plates per strain) containing agar Chrome Azurol S (CAS) and incubated at 30 &#8451. Plates were observed daily for 7 d to detect the appearance of an orange halo around the colonies. Phosphate solubilization: To determine the phosphate-solubilizing activity, the isolates were cultured in triplicate in modified Pikovskayas medium (0.5% tricalcium phosphate) at 30 &#8451 for 7 d at 200 rpm. Indole acetic acid (IAA) production: Briefly, each bacterial suspension (1 × 108 cfu/mL) was inoculated in 10 mL LB broth containing L-TRYPTOPHAN (100 µG/ML) AND INCUBATED AT 28 &#8451 for 72 h at 200 rpm. Bacterial cells were removed by centrifugation at 8,000 rpm for 15 min, and the collected supernatant was incubated at room temperature in the dark for 30 min.
Click to Show/Hide
            Phosphate Solubilization [1]
               Factor Link Part Location NP Content
 
Modified Pikovskayas Medium with 0.5% tricalcium phosphate(30 degrees Celsius + 7Days)
   NP Info    root, stem, or leaf Henan and Shandong Provinces, China
NP Content: 18.3 mg/L
      Species Name: Paenibacillus ehimensis strain HNSQJYH130
          Species Info Click to show the detail information of this Factor
          Experiment Detail
Root, stem, and leaf samples were collected from the medicinal plant L. japonica (traditional variety Damaohua, 2n = 18) growing in four different locations in the Henan and Shandong Provinces from July 2011 to July 2012. Samples were collected during the plant's flowering and growth stages, when active metabolism facilitated plant identification. The four sampling sites were the medicinal botanical garden of Shangqiu Normal University, the Huaxian County of Henan Province, and the Pingyi County and Juye County of Shangdong Province. Examination of siderophore production: Bacteria were cultured in lysogeny broth (LB; 10 g NaCl/L) for 72 h under iron-restricted conditions. Aliquots of each bacterial culture were inoculated in plates (three plates per strain) containing agar Chrome Azurol S (CAS) and incubated at 30 &#8451. Plates were observed daily for 7 d to detect the appearance of an orange halo around the colonies. Phosphate solubilization: To determine the phosphate-solubilizing activity, the isolates were cultured in triplicate in modified Pikovskayas medium (0.5% tricalcium phosphate) at 30 &#8451 for 7 d at 200 rpm. Indole acetic acid (IAA) production: Briefly, each bacterial suspension (1 × 108 cfu/mL) was inoculated in 10 mL LB broth containing L-TRYPTOPHAN (100 µG/ML) AND INCUBATED AT 28 &#8451 for 72 h at 200 rpm. Bacterial cells were removed by centrifugation at 8,000 rpm for 15 min, and the collected supernatant was incubated at room temperature in the dark for 30 min.
Click to Show/Hide
            Phosphate Solubilization [1]
               Factor Link Part Location NP Content
 
Modified Pikovskayas Medium with 0.5% tricalcium phosphate(30 degrees Celsius + 7Days)
   NP Info    root, stem, or leaf Henan and Shandong Provinces, China
NP Content: 0.9 mg/L
      Species Name: Paenibacillus polymyxa strain HNSQJYH122
          Species Info Click to show the detail information of this Factor
          Experiment Detail
Root, stem, and leaf samples were collected from the medicinal plant L. japonica (traditional variety Damaohua, 2n = 18) growing in four different locations in the Henan and Shandong Provinces from July 2011 to July 2012. Samples were collected during the plant's flowering and growth stages, when active metabolism facilitated plant identification. The four sampling sites were the medicinal botanical garden of Shangqiu Normal University, the Huaxian County of Henan Province, and the Pingyi County and Juye County of Shangdong Province. Examination of siderophore production: Bacteria were cultured in lysogeny broth (LB; 10 g NaCl/L) for 72 h under iron-restricted conditions. Aliquots of each bacterial culture were inoculated in plates (three plates per strain) containing agar Chrome Azurol S (CAS) and incubated at 30 &#8451. Plates were observed daily for 7 d to detect the appearance of an orange halo around the colonies. Phosphate solubilization: To determine the phosphate-solubilizing activity, the isolates were cultured in triplicate in modified Pikovskayas medium (0.5% tricalcium phosphate) at 30 &#8451 for 7 d at 200 rpm. Indole acetic acid (IAA) production: Briefly, each bacterial suspension (1 × 108 cfu/mL) was inoculated in 10 mL LB broth containing L-TRYPTOPHAN (100 µG/ML) AND INCUBATED AT 28 &#8451 for 72 h at 200 rpm. Bacterial cells were removed by centrifugation at 8,000 rpm for 15 min, and the collected supernatant was incubated at room temperature in the dark for 30 min.
Click to Show/Hide
            Phosphate Solubilization [1]
               Factor Link Part Location NP Content
 
Modified Pikovskayas Medium with 0.5% tricalcium phosphate(30 degrees Celsius + 7Days)
   NP Info    root, stem, or leaf Henan and Shandong Provinces, China
NP Content: 41.7 mg/L

References
1 Screening and characterization of endophytic Bacillus and Paenibacillus strains from medicinal plant Lonicera japonica for use as potential plant growth promoters