The strain Actinosynnema pretiosum FIM06-0063 from Fujian Institute of Microbiology was cultivated on solid state ISP4 media and incubated in 1.5% soluble starch, 0.5% glucose, 1% soybean flour, 0.5% peptone, 0.1% NaCl, 0.5% yeast extract and 0.5% CaCO₃, pH 7.0 medium at 28 ℃ for 24 hours on a rotary shaker (250 r/min ). In the progress of fermention, 6 mL of the vegetative culture were transferred to 250 mL flasks containing 60 mL production medium (1% dextrin, 3% glycerol, 2% soybean flour, 1% corn steep liquor, 0.05% KH₂PO₄, 0.05% NaCl and 0.5% CaCO₃ pH = 7.0) and incubated at 28 ℃ for 5 days on a rotary shaker (250 r/min ).