The seeds and roots of five adult healthy plants of P. cupana from each clonal cultivars CMU 871 (tolerant to anthracnose and oversprouting) and CMU 300 (tolerant to anthracnose and susceptible to oversprouting) were collected in November 2014 in the Embrapa (Brazilian Agricultural Research Company) experimental farms located in the cities of Manaus (2° 53′ 29.14″ S and 59° 58′ 39.90″ W, 99 m high) and Maues (3° 22′ 54″ S and 57° 42′ 55″ W, 18 m high), State of Amazonas, Brazil. The plant material was collected at the end of the dry season, when the accumulated rainfall and average temperature were respectively 196.0 mm and 28.46 ℃ in Manaus, and 272.3 mm and 28.17 ℃ in Maues. Five millimeter diameter plugs of each endophytic fungus were place at the center of Petri dishes (90 mm diameter), each containing 20 mL of PDA, and cultured for 13 days at 25 ℃.