The fungus P. multirostrata EA-12 was isolated from fresh leaves of E. Adenophorum collected in Kunming Institute of Botany, Kunming, Yunnan province, PR China in July 2012. This fungal strain was cultured on potato dextrose agar medium at 25 ℃ for 10 days. The agar plugs were inoculated in 500-ml Erlenmeyer flasks, each containing 100 ml of potato dextrose media. Flask cultures were incubated at 28 ℃ on a rotary shaker at 160r.p.m. for 2 days as seed culture. Sixty 500-ml Erlenmeyer flasks each containing 150 ml of potato dextrose broth were individually inoculated with 25′l of seed culture and incubated at 25 ℃ on a rotary shaker at 160r.p.m. for 15 days.