Fungal strain G536 was isolated from surface sterilized twigs of paw paw (A. triloba (L.) Dunal, Annonaceae) collected from Pfafftown, NC, USA (36°09′ 58.8″ N 80°24′18.6″W). In preparation for chemical extraction, G536 was grown on rice, as this has been shown to be an efficient medium for the production of secondary metabolites in culture. To make seed cultures for inoculating rice, a piece of fresh culture grown in potato dextrose (PD) (Difco) or 2% malt extract (ME) (Difco) media was excised from the leading edge of the colony and transferred to a liquid seed medium containing 2% soy peptone, 2% dextrose, and 1% yeast extract (YESD; 5 g of yeast extract, 10 g of soy peptone, and 10 g of d-glucose in 500 ml distilled water). Following incubation for 7 days at 22°C with agitation, the culture was used to inoculate 10 g of rice media prepared using 30 ml of distilled H₂O and autoclaved in a 250 ml Erlenmeyer flask. This screener scale fermentation was incubated at 22°C for 14-21 days prior to chemical extraction. For large-scale production of fungal cultures, four 250 ml Erlenmeyer flasks containing 10 g of rice were inoculated using one seed culture for each flask.