General Information of Species (ID: SP0190)
  Species Name
Alternaria infectoria
  Species Genus Alternaria
  Species Family Pleosporaceae
  Microbe Type Endophytic Fungi
  Studied Organism infectoria sp. SNB-GTC2402
  External Links
Taxonomy ID 1347960
Genbank ID KF164320

 The Content Variation of Natural Product Induced by Different Factor(s)
      PDA medium [1]
              Factor Info Click to show the detail information of this Factor
               Experiment Detail
Isolation of endophytes: The leaves of 24 plant species were collected in the Amazon Rainforest biome, in Cayenne and Roura, French Guiana. After collection, the plant material was washed with sterile water and surface sterilised by sequential immersion in 70% aqueous ethanol (3 min), followed by 5% aqueous sodium hypochlorite (5 min) and finally by 70% aqueous ethanol (1 min). After these procedures, the leaves were rinsed with sterilised water. The residual sterilised water was incubated in Petri dishes to ensure that all the epiphytic microorganisms were eliminated. The surface-sterilised leaves were cut into 64 small pieces which were placed in 2 ml Eppendorf tubes containing 1 ml of potato dextrose agar medium (PDA, Fluka Analytical, Germany) at 26 ℃. Each individual hyphal tip of emerging fungi was removed and placed on a sterile PDA culture medium in 10 cm Petri dishes. The leaf fragments were cultured for a maximum of 5 months. All strains, including bacteria, were cultivated on solid PDA medium at 26 ℃ for 15 days, on 3 Petri dishes of 14 cm diameter (150 cm2). On the large scale, the microorganisms were cultivated under the identical conditions with 130 Petri dishes of 14 cm diameter (2 m2).
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  PDA medium (26℃ + 15 days)   (Part: Leaves; Location: Cayenne and Roura, French Guiana)
               NP Name Link Host Plant Part Location NP Content
 
Pyrrocidine C
NP Info . Leaves Cayenne and Roura, French Guiana
NP Content: 5 mg
 
Alteichin
NP Info . Leaves Cayenne and Roura, French Guiana
NP Content: 50.3 mg
 
Novaezelandin B
NP Info . Leaves Cayenne and Roura, French Guiana
NP Content: 5.3 mg
 
Pyrenocine A
NP Info . Leaves Cayenne and Roura, French Guiana
NP Content: 3.6 mg
References
1 Antimicrobial and cytotoxic secondary metabolites from tropical leaf endophytes: Isolation of antibacterial agent pyrrocidine C from Lewia infectoria SNB-GTC2402