| General Information of Species (ID: SP0253) | |||||||
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| Species Name |
Streptomyces sp.
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| Species Genus | Streptomyces | ||||||
| Species Family | Streptomycetaceae | ||||||
| Microbe Type | Endophytic Actinomycetes | ||||||
| Studied Organism | Streptomyces sp. strain GT20021503 | ||||||
| External Links | |||||||
| Taxonomy ID | 1931 | ||||||
| Host Plant (s) | |||||||
| Bruguiera gymnorrhiza | Species Info | ||||||
| The Content Variation of Natural Product Induced by Different Factor(s) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Fermentation medium | [1] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Factor Info
Click to show the detail information of this Factor
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| Experiment Detail |
Streptomyces sp. GT20021503 was isolated from the stem of B. gymnorrhiza. Liquid organic medium 79 (dextrose 10 g, bacto peptone 10 g, casamino acids 1 g, yeast extract 2 g, NaCl 6 g, H2O 1 L) (2 × 100 mL/flask) was inoculated with a suspension of mycelium and spores (about 1 × 1 cm) of the title strain grown on agar slants or agar plates (oatmeal 20 g, agar 18 g, H2O 1 L, pH 7.2,). After incubation for 48 h on a rotary shaker at 28 ℃, the culture was transferred to 3200 mL of medium 2 (oatmeal 20 g, CaCl2 5.7 × 10-3 g, Fe-citrate 2.5 × 10-3 g, MnSO4 5.0 × 10-4 g, ZnCl2 2.5 × 10-4 g, CuSO4 4.0 × 10-5 g, Na2B4O2 5.0 × 10-5g, CoCl 1.0 × 10-5 g, NaMoO4 2.1x 10-5g, H2O 1 L, pH 7.8) (eight 1000 mL-scale Erlenmeyer flasks with 400 mL of medium 2 each) and incubated at 28 ℃ under shaking conditions for 48 h to yield prefermentation culture, which was poured into a 300 L-scale fermenter filled with 200 L of medium 2 and fermented for 5 days.
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| Fermentation medium containing oatmeal 20 g, CaCl22 5.7 × 10-3 g, Fe-citrate 2.5 × 10-3 g, MnSO4 5.0 × 10-4 g, ZnCl22 2.5 × 10-4 g, CuSO4 4.0 × 10-5 g, Na2B4O2 5.0 × 10-5g, CoCl 1.0 × 10-5 g, NaMoO4 2.1x 10-5g, H2O 1 L (28℃ + 5 days) (Part: Stems; Location: .) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| NP Name | Link | Host | Plant Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Xiamycin Methyl Ester
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NP Info | Bruguiera gymnorrhiza | Stems | NA |
NP Content: 1.5 mg
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Xiamycin
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NP Info | Bruguiera gymnorrhiza | Stems | NA |
NP Content: 50 mg
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| References | ||||||||||||||||||||||||
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| 1 | Xiamycin, a pentacyclic indolosesquiterpene with selective anti-HIV activity from a bacterial mangrove endophyte | |||||||||||||||||||||||