| The Content Variation of Natural Product Induced by Different Factor(s) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| MGs medium | [1] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Factor Info
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| Experiment Detail |
Soil samples were collected from 16 medicinal plant rhizospheres in Lumphun Province. Indole acetic acid (IAA) production: The actinomycete discs (8 mm), grown on yeast malt extract agar (YM) incubated at 28 ℃ for 5 days, were inoculated into 5 ml YM broth containing 0.2% L-tryptophan and incubated at 28 ℃ with shaking at 125 rev/min for 7 days. Cultures were centrifuged at 11,000 rev/min for 15 min. One milliliter of the supernatant was mixed with 2 ml of Salkowski reagent. Appearance of a pink color indicated IAA production. Optical density (OD) was read at 530 nm using a spectrophotometer. The level of IAA produced was estimated by comparison with an IAA standard. The actinomycete discs (8 mm), grown on YM agar incubated at 28 ℃ for 5 days were inoculated on CAS-substrates with modified Gaus No.1 medium (MGs) and incubated at 28 ℃ for 10 days. The colonies with orange zones were considered as siderophore-producing isolates. The functional groups of the siderophores were determined. The active isolates (width of orange zone on CAS plate >20 mm) were cultured on modified Gaus No.1 broth and incubated at 28 ℃ with shaking at 125 rpm for 10 days. Catechol-type siderophores were estimated by Arnow's method (Arnow 1937) and hydroxamate siderophores were estimated by the Csaky test (Csaky 1948).
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| MGs medium with CAS-substrates (28℃ + 10 days) (Part: Soil samples were collected from rhizospheres; Location: Lumphun Province, Thailand) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| NP Name | Link | Host | Plant Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Hydroxamate Siderophores
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NP Info | . | Soil samples were collected from rhizospheres | Lumphun Province, Thailand |
NP Content: 52.42 ± 0.3 µg/ml
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| References | ||||||||||||||||||||||||
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| 1 | Actinomycetes isolated from medicinal plant rhizosphere soils: diversity and screening of antifungal compounds, indole-3-acetic acid and siderophore production | |||||||||||||||||||||||
