Details of Species

 General Information of Species (ID: SP0461)
  Species Name
Aspergillus aculeatinus
  Species Genus Aspergillus
  Species Family Aspergillaceae
  Microbe Type Endophytic Fungi
  Studied Organism Aspergillus aculeatinus Tax-6
  External Links
Taxonomy ID 487661
Genbank ID KU378102
  Host Plant (s)
Taxus chinensis Species Info

 The Content Variation of Natural Product Induced by Different Factor(s)
      PDB medium [1]
              Factor Info Click to show the detail information of this Factor
               Experiment Detail
Fungi were isolated from the outer and inner bark of Taxus chinensis var. maire in Baiji, Anhui Province, China. The endophytic fungus was aerobically cultured in potato dextrose broth medium at 28 ℃ for one week. Plackett-Burman design: In this study, seven factors were included. For each factor, a high (+) and low (-) level was tested. Twelve runs of various levels of factors were formulated by Design-Expert version 8.0 and the response was measured according to the yield of taxol. All runs were conducted in 250 mL Erlenmeyer flasks containing 100 mL PDA liquid medium at 30 ℃ with an initial pH of 7.0. The tested factors were: sodium benzoate; salicylic acid; phenylalanine; sodium acetate; glycine; CuSO4; methyl jasmonate. After culturing for 8 days, the fungal taxol was extracted as described above.
Click to Show/Hide
  PDB medium (28℃ + 7 days)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 334.92 µg/L
      PDA medium [1]
              Factor Info Click to show the detail information of this Factor
               Experiment Detail
Fungi were isolated from the outer and inner bark of Taxus chinensis var. maire in Baiji, Anhui Province, China. The endophytic fungus was aerobically cultured in potato dextrose broth medium at 28 ℃ for one week. Plackett-Burman design: In this study, seven factors were included. For each factor, a high (+) and low (-) level was tested. Twelve runs of various levels of factors were formulated by Design-Expert version 8.0 and the response was measured according to the yield of taxol. All runs were conducted in 250 mL Erlenmeyer flasks containing 100 mL PDA liquid medium at 30 ℃ with an initial pH of 7.0. The tested factors were: sodium benzoate; salicylic acid; phenylalanine; sodium acetate; glycine; CuSO4; methyl jasmonate. After culturing for 8 days, the fungal taxol was extracted as described above.
Click to Show/Hide
  PDA medium with 10, 15, 8, 25, 0.15 mg/L sodium benzoate, salicylic acid, phenylalanine, glycine, CuSO4 and 6 g/L sodium acetate, 150 µmol/L methyl jasmonate model (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 492.48 µg/L
  PDA medium with 20, 5, 2, 25, 0.05 mg/L sodium benzoate, salicylic acid, phenylalanine, glycine, CuSO4 and 6 g/L sodium acetate, 150 µmol/L methyl jasmonate model (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 493.81 µg/L
  PDA medium with 20, 5, 8, 15, 0.15 mg/L sodium benzoate, salicylic acid, phenylalanine, glycine, CuSO4 and 10 g/L sodium acetate, 150 µmol/L methyl jasmonate model (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 468.54 µg/L
  PDA medium with 20, 15, 2, 15, 0.15 mg/L sodium benzoate, salicylic acid, phenylalanine, glycine, CuSO4 and 6 g/L sodium acetate, 150 µmol/L methyl jasmonate model (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 822.1 µg/L
  PDA medium with 10, 5, 2, 15, 0.05 mg/L sodium benzoate, salicylic acid, phenylalanine, glycine, CuSO4 and 6 g/L sodium acetate, 50 µmol/L methyl jasmonate model (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 355.23 µg/L
  PDA medium with 10, 15, 2, 25, 0.05 mg/L sodium benzoate, salicylic acid, phenylalanine, glycine, CuSO4 and 10 g/L sodium acetate, 150 µmol/L methyl jasmonate model (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 365.25 µg/L
  PDA medium with 10, 5, 8, 25, 0.15 mg/L sodium benzoate, salicylic acid, phenylalanine, glycine, CuSO4 and 6 g/L sodium acetate, 50 µmol/L methyl jasmonate model (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 384.26 µg/L
  PDA medium with 20, 15, 2, 25, 0.15 mg/L sodium benzoate, salicylic acid, phenylalanine, glycine, CuSO4 and 10 g/L sodium acetate, 50 µmol/L methyl jasmonate model (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 851.25 µg/L
  PDA medium with 10, 15, 8, 15, 0.05 mg/L sodium benzoate, salicylic acid, phenylalanine, glycine, CuSO4 and 10 g/L sodium acetate, 50 µmol/L methyl jasmonate model (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 404.52 µg/L
  PDA medium with 20, 5, 8, 25, 0.05 mg/L sodium benzoate, salicylic acid, phenylalanine, glycine, CuSO4 and 10 g/L sodium acetate, 50 µmol/L methyl jasmonate model (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 410.45 µg/L
  PDA medium with 20, 15, 8, 15, 0.05 mg/L sodium benzoate, salicylic acid, phenylalanine, glycine, CuSO4 and 6 g/L sodium acetate, 150 µmol/L methyl jasmonate model (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 502.54 µg/L
  PDA medium with 10, 5, 2, 15, 0.15 mg/L sodium benzoate, salicylic acid, phenylalanine, glycine, CuSO4 and 10 g/L sodium acetate, 150 µmol/L methyl jasmonate model (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 305.65 µg/L
  PDA medium with 0.05 mg/L CuSO4, 5 mg/L salicylic acid and 8 g/L sodium acetate (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 335.28 µg/L
  PDA medium with 0.15 mg/L CuSO4, 5 mg/L salicylic acid and 10 g/L sodium acetate (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 476.73 µg/L
  PDA medium with 0.05 mg/L CuSO4, 15 mg/L salicylic acid and 8 g/L sodium acetate (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 454.64 µg/L
  PDA medium with 0.15 mg/L CuSO4, 15 mg/L salicylic acid and 8 g/L sodium acetate (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 282.62 µg/L
  PDA medium with 0.05 mg/L CuSO4, 10 mg/L salicylic acid and 6 g/L sodium acetate (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 413.73 µg/L
  PDA medium with 0.15 mg/L CuSO4, 10 mg/L salicylic acid and 6 g/L sodium acetate (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 547.26 µg/L
  PDA medium with 0.05 mg/L CuSO4, 10 mg/L salicylic acid and 10 g/L sodium acetate (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 854.9 µg/L
  PDA medium with 0.15 mg/L CuSO4, 10 mg/L salicylic acid and 10 g/L sodium acetate (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 843.26 µg/L
  PDA medium with 0.1 mg/L CuSO4, 5 mg/L salicylic acid and 6 g/L sodium acetate (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 633.38 µg/L
  PDA medium with 0.1 mg/L CuSO4, 15 mg/L salicylic acid and 6 g/L sodium acetate (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 338.24 µg/L
  PDA medium with 0.1 mg/L CuSO4, 5 mg/L salicylic acid and 10 g/L sodium acetate (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 565.72 µg/L
  PDA medium with 0.1 mg/L CuSO4, 15 mg/L salicylic acid and 10 g/L sodium acetate (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 828.8 µg/L
  PDA medium with 0.1 mg/L CuSO4, 10 mg/L salicylic acid and 8 g/L sodium acetate (30℃ + 8 days + PH7)   (Part: Outer and inner bark; Location: Baiji, Anhui Province, China)
               NP Name Link Host Plant Part Location NP Content
 
Paclitaxel
 NP Info Taxus chinensis Outer and inner bark Baiji, Anhui Province, China
NP Content: 1377.56 µg/L
References
1 Enhancing taxol production in a novel endophytic fungus, Aspergillus aculeatinus Tax-6, isolated from Taxus chinensis var. mairei