Details of Factor

General Information of Factor (ID: FP084)
  Factor Name Millet Medium
  Factor Type Environmental Conditions
  Factor Description
Each microbial strain has the potential to produce multiple compounds, but only subsets of these compounds are made under specific growth conditions. Therefore, variations in cultivation parameters can elicit the production and discovery of new secondary metabolites by changing cultivation parameters such as media composition, various nutrients, trace elements, physical parameters (i.e., pH, temperature), and chemical elicitors (i.e., sub-lethal concentrations of antibiotics, communication molecules). Moreover, the co-cultivation of microbes and the addition of factors affecting epigenetic control can also be framed within the OSMAC principle.
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 The Content Variation of Natural Product Induced by This Factor
      Species Name: Cephalosporium sp. IFB-E001
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          Experiment Detail
A total of 1626 cultivable endophytic fungal isolates were obtained from the surface-sterilized fresh tissues of apparently healthy wild vines of T. jasminoides after collection in November (2001), April, and June (2002) from the eastern suburbs of Nanjing, China. The culture liquor (20 ml) of the endophyte Cephalosporium sp. IFB-E001 was applied to solid-matrix steady fermentation using millet medium (millet 7.5 g, bran 7.5 g, yeast extract 0.5 g, FeSO4 0.01 g, tartrate sodium 0.1 g, glutamine sodium 0.1 g and pure corn oil 0.1 ml in tap water 15 ml per bottle). The fermentation, accomplished in a total of 420 bottles, was subsequently accomplished by standing for another 30 d at 28 ℃.
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            Graphislactone A [1]
               Factor Link Part Location NP Content
 
Millet medium (28℃ + 30 days)
    NP Info    Wild vines Nanjing, China
NP Content: 13 mg

References
1 Characterization of graphislactone A as the antioxidant and free radical-scavenging substance from the culture of Cephalosporium sp. IFB-E001, an endophytic fungus in Trachelospermum jasminoides