General Information of Factor (ID: FP109)
  Factor Name Csaky Assay
  Factor Type Environmental Conditions
  Factor Description
Each microbial strain has the potential to produce multiple compounds, but only subsets of these compounds are made under specific growth conditions. Therefore, variations in cultivation parameters can elicit the production and discovery of new secondary metabolites by changing cultivation parameters such as media composition, various nutrients, trace elements, physical parameters (i.e., pH, temperature), and chemical elicitors (i.e., sub-lethal concentrations of antibiotics, communication molecules). Moreover, the co-cultivation of microbes and the addition of factors affecting epigenetic control can also be framed within the OSMAC principle.
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 The Content Variation of Natural Product Induced by This Factor
      Species Name: Streptomyces collinus strain SP8
          Species Info Click to show the detail information of this Factor
          Experiment Detail
Healthy medicinal plants were collected from CSIR-National Botanical Research Institute (Banthara) (26° 55′ N, 80° 59′ E), Lucknow, India during June, 2013. Catechol type and hydroxamate siderophores were estimated by Arnow's method (Arnow 1937) and Csaky test (Csaky 1948) respectively. The amount of IAA produced by Actinomycetes isolates was determined using GYM broth supplemented with different concentration of l-tryptophan (Basal, 0, 2 and 5 mg/ml) and incubated at 30 ℃ with shaking at 125 rev/min for 7 days (Gordon and Weber 1951). Appearance of a pink colour indicated IAA production. The level of IAA produced was estimated by comparison with an IAA standard. Gibberellic acid (GA3) production was determined according to Borrow et al. (1955)(In submerged aerated culture on Raulin-Thom medium (4% sucrose) in 18 days at 25 ℃.).
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            Hydroxamate Siderophores [1]
               Factor Link Part Location NP Content
 
Csaky assay (Assay for hydroxamate siderophore)
   NP Info    Roots Lucknow, India
NP Content: 39.55 ± 1.43 µg/ml
      Species Name: Streptomyces diastaticus strain SP2
          Species Info Click to show the detail information of this Factor
          Experiment Detail
Healthy medicinal plants were collected from CSIR-National Botanical Research Institute (Banthara) (26° 55′ N, 80° 59′ E), Lucknow, India during June, 2013. Catechol type and hydroxamate siderophores were estimated by Arnow's method (Arnow 1937) and Csaky test (Csaky 1948) respectively. The amount of IAA produced by Actinomycetes isolates was determined using GYM broth supplemented with different concentration of l-tryptophan (Basal, 0, 2 and 5 mg/ml) and incubated at 30 ℃ with shaking at 125 rev/min for 7 days (Gordon and Weber 1951). Appearance of a pink colour indicated IAA production. The level of IAA produced was estimated by comparison with an IAA standard. Gibberellic acid (GA3) production was determined according to Borrow et al. (1955)(In submerged aerated culture on Raulin-Thom medium (4% sucrose) in 18 days at 25 ℃.).
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            Hydroxamate Siderophores [1]
               Factor Link Part Location NP Content
 
Csaky assay (Assay for hydroxamate siderophore)
   NP Info    Roots Lucknow, India
NP Content: 41.87 ± 3.89 µg/ml
      Species Name: Streptomyces fradiae strain SP4
          Species Info Click to show the detail information of this Factor
          Experiment Detail
Healthy medicinal plants were collected from CSIR-National Botanical Research Institute (Banthara) (26° 55′ N, 80° 59′ E), Lucknow, India during June, 2013. Catechol type and hydroxamate siderophores were estimated by Arnow's method (Arnow 1937) and Csaky test (Csaky 1948) respectively. The amount of IAA produced by Actinomycetes isolates was determined using GYM broth supplemented with different concentration of l-tryptophan (Basal, 0, 2 and 5 mg/ml) and incubated at 30 ℃ with shaking at 125 rev/min for 7 days (Gordon and Weber 1951). Appearance of a pink colour indicated IAA production. The level of IAA produced was estimated by comparison with an IAA standard. Gibberellic acid (GA3) production was determined according to Borrow et al. (1955)(In submerged aerated culture on Raulin-Thom medium (4% sucrose) in 18 days at 25 ℃.).
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            Hydroxamate Siderophores [1]
               Factor Link Part Location NP Content
 
Csaky assay (Assay for hydroxamate siderophore)
   NP Info    Roots Lucknow, India
NP Content: 27.36 ± 1.42 µg/ml
      Species Name: Streptomyces griseus strain SP12
          Species Info Click to show the detail information of this Factor
          Experiment Detail
Healthy medicinal plants were collected from CSIR-National Botanical Research Institute (Banthara) (26° 55′ N, 80° 59′ E), Lucknow, India during June, 2013. Catechol type and hydroxamate siderophores were estimated by Arnow's method (Arnow 1937) and Csaky test (Csaky 1948) respectively. The amount of IAA produced by Actinomycetes isolates was determined using GYM broth supplemented with different concentration of l-tryptophan (Basal, 0, 2 and 5 mg/ml) and incubated at 30 ℃ with shaking at 125 rev/min for 7 days (Gordon and Weber 1951). Appearance of a pink colour indicated IAA production. The level of IAA produced was estimated by comparison with an IAA standard. Gibberellic acid (GA3) production was determined according to Borrow et al. (1955)(In submerged aerated culture on Raulin-Thom medium (4% sucrose) in 18 days at 25 ℃.).
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            Hydroxamate Siderophores [1]
               Factor Link Part Location NP Content
 
Csaky assay (Assay for hydroxamate siderophore)
   NP Info    Roots Lucknow, India
NP Content: 53.58 ± 2.49 µg/ml
      Species Name: Streptomyces olivochromogenes strain SP5
          Species Info Click to show the detail information of this Factor
          Experiment Detail
Healthy medicinal plants were collected from CSIR-National Botanical Research Institute (Banthara) (26° 55′ N, 80° 59′ E), Lucknow, India during June, 2013. Catechol type and hydroxamate siderophores were estimated by Arnow's method (Arnow 1937) and Csaky test (Csaky 1948) respectively. The amount of IAA produced by Actinomycetes isolates was determined using GYM broth supplemented with different concentration of l-tryptophan (Basal, 0, 2 and 5 mg/ml) and incubated at 30 ℃ with shaking at 125 rev/min for 7 days (Gordon and Weber 1951). Appearance of a pink colour indicated IAA production. The level of IAA produced was estimated by comparison with an IAA standard. Gibberellic acid (GA3) production was determined according to Borrow et al. (1955)(In submerged aerated culture on Raulin-Thom medium (4% sucrose) in 18 days at 25 ℃.).
Click to Show/Hide
            Hydroxamate Siderophores [1]
               Factor Link Part Location NP Content
 
Csaky assay (Assay for hydroxamate siderophore)
   NP Info    Roots Lucknow, India
NP Content: 25.57 ± 2.78 µg/ml
      Species Name: Streptomyces ossamyceticus strain SP10
          Species Info Click to show the detail information of this Factor
          Experiment Detail
Healthy medicinal plants were collected from CSIR-National Botanical Research Institute (Banthara) (26° 55′ N, 80° 59′ E), Lucknow, India during June, 2013. Catechol type and hydroxamate siderophores were estimated by Arnow's method (Arnow 1937) and Csaky test (Csaky 1948) respectively. The amount of IAA produced by Actinomycetes isolates was determined using GYM broth supplemented with different concentration of l-tryptophan (Basal, 0, 2 and 5 mg/ml) and incubated at 30 ℃ with shaking at 125 rev/min for 7 days (Gordon and Weber 1951). Appearance of a pink colour indicated IAA production. The level of IAA produced was estimated by comparison with an IAA standard. Gibberellic acid (GA3) production was determined according to Borrow et al. (1955)(In submerged aerated culture on Raulin-Thom medium (4% sucrose) in 18 days at 25 ℃.).
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            Hydroxamate Siderophores [1]
               Factor Link Part Location NP Content
 
Csaky assay (Assay for hydroxamate siderophore)
   NP Info    Roots Lucknow, India
NP Content: 11.73 ± 1.82 µg/ml

References
1 Evaluation of antagonistic and plant growth promoting activities of chitinolytic endophytic actinomycetes associated with medicinal plants against Sclerotium rolfsii in chickpea