General Information of Species (ID: SP0410)
  Species Name
Streptomyces fradiae
  Species Genus Streptomyces
  Species Family Streptomycetaceae
  Microbe Type Endophytic Actinomycetes
  Studied Organism Streptomyces fradiae strain SP4
  External Links
Taxonomy ID 1906
Genbank ID KT962909
  Host Plant (s)
Withania somnifera Species Info

 The Content Variation of Natural Product Induced by Different Factor(s)
      Arnow's assays [1]
              Factor Info Click to show the detail information of this Factor
               Experiment Detail
Healthy medicinal plants were collected from CSIR-National Botanical Research Institute (Banthara) (26° 55′ N, 80° 59′ E), Lucknow, India during June, 2013. Catechol type and hydroxamate siderophores were estimated by Arnow's method (Arnow 1937) and Csaky test (Csaky 1948) respectively. The amount of IAA produced by Actinomycetes isolates was determined using GYM broth supplemented with different concentration of l-tryptophan (Basal, 0, 2 and 5 mg/ml) and incubated at 30 ℃ with shaking at 125 rev/min for 7 days (Gordon and Weber 1951). Appearance of a pink colour indicated IAA production. The level of IAA produced was estimated by comparison with an IAA standard. Gibberellic acid (GA3) production was determined according to Borrow et al. (1955)(In submerged aerated culture on Raulin-Thom medium (4% sucrose) in 18 days at 25 ℃.).
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  Arnow's assays (Assay for catecholate siderophore)   (Part: Roots; Location: Lucknow, India)
               NP Name Link Host Plant Part Location NP Content
 
Catechol-Type Siderophores
NP Info Withania somnifera Roots Lucknow, India
NP Content: 9.45 ± 0.74 µg/ml
      Csaky assay [1]
              Factor Info Click to show the detail information of this Factor
               Experiment Detail
Healthy medicinal plants were collected from CSIR-National Botanical Research Institute (Banthara) (26° 55′ N, 80° 59′ E), Lucknow, India during June, 2013. Catechol type and hydroxamate siderophores were estimated by Arnow's method (Arnow 1937) and Csaky test (Csaky 1948) respectively. The amount of IAA produced by Actinomycetes isolates was determined using GYM broth supplemented with different concentration of l-tryptophan (Basal, 0, 2 and 5 mg/ml) and incubated at 30 ℃ with shaking at 125 rev/min for 7 days (Gordon and Weber 1951). Appearance of a pink colour indicated IAA production. The level of IAA produced was estimated by comparison with an IAA standard. Gibberellic acid (GA3) production was determined according to Borrow et al. (1955)(In submerged aerated culture on Raulin-Thom medium (4% sucrose) in 18 days at 25 ℃.).
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  Csaky assay (Assay for hydroxamate siderophore)   (Part: Roots; Location: Lucknow, India)
               NP Name Link Host Plant Part Location NP Content
 
Hydroxamate Siderophores
NP Info Withania somnifera Roots Lucknow, India
NP Content: 27.36 ± 1.42 µg/ml
      GYM medium [1]
              Factor Info Click to show the detail information of this Factor
               Experiment Detail
Healthy medicinal plants were collected from CSIR-National Botanical Research Institute (Banthara) (26° 55′ N, 80° 59′ E), Lucknow, India during June, 2013. Catechol type and hydroxamate siderophores were estimated by Arnow's method (Arnow 1937) and Csaky test (Csaky 1948) respectively. The amount of IAA produced by Actinomycetes isolates was determined using GYM broth supplemented with different concentration of l-tryptophan (Basal, 0, 2 and 5 mg/ml) and incubated at 30 ℃ with shaking at 125 rev/min for 7 days (Gordon and Weber 1951). Appearance of a pink colour indicated IAA production. The level of IAA produced was estimated by comparison with an IAA standard. Gibberellic acid (GA3) production was determined according to Borrow et al. (1955)(In submerged aerated culture on Raulin-Thom medium (4% sucrose) in 18 days at 25 ℃.).
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  GYM medium with 0 mg/ml L-tryptophan (30℃ + 7 days)   (Part: Roots; Location: Lucknow, India)
               NP Name Link Host Plant Part Location NP Content
 
Indole-3-Acetic Acid
NP Info Withania somnifera Roots Lucknow, India
NP Content: 5.99 ± 0.13 µg/ml
  GYM medium with 2 mg/ml L-tryptophan (30℃ + 7 days)   (Part: Roots; Location: Lucknow, India)
               NP Name Link Host Plant Part Location NP Content
 
Indole-3-Acetic Acid
NP Info Withania somnifera Roots Lucknow, India
NP Content: 25.55 ± 1.28 µg/ml
  GYM medium with 5 mg/ml L-tryptophan (30℃ + 7 days)   (Part: Roots; Location: Lucknow, India)
               NP Name Link Host Plant Part Location NP Content
 
Indole-3-Acetic Acid
NP Info Withania somnifera Roots Lucknow, India
NP Content: 45.83 ± 1.82 µg/ml
      Raulin-Thom medium [1]
              Factor Info Click to show the detail information of this Factor
               Experiment Detail
Healthy medicinal plants were collected from CSIR-National Botanical Research Institute (Banthara) (26° 55′ N, 80° 59′ E), Lucknow, India during June, 2013. Catechol type and hydroxamate siderophores were estimated by Arnow's method (Arnow 1937) and Csaky test (Csaky 1948) respectively. The amount of IAA produced by Actinomycetes isolates was determined using GYM broth supplemented with different concentration of l-tryptophan (Basal, 0, 2 and 5 mg/ml) and incubated at 30 ℃ with shaking at 125 rev/min for 7 days (Gordon and Weber 1951). Appearance of a pink colour indicated IAA production. The level of IAA produced was estimated by comparison with an IAA standard. Gibberellic acid (GA3) production was determined according to Borrow et al. (1955)(In submerged aerated culture on Raulin-Thom medium (4% sucrose) in 18 days at 25 ℃.).
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  Raulin-Thom medium (25℃ + 18 days)   (Part: Roots; Location: Lucknow, India)
               NP Name Link Host Plant Part Location NP Content
 
Gibberellic Acid
NP Info Withania somnifera Roots Lucknow, India
NP Content: 5.74 ± 0.72 µg/ml
References
1 Evaluation of antagonistic and plant growth promoting activities of chitinolytic endophytic actinomycetes associated with medicinal plants against Sclerotium rolfsii in chickpea