Collection of the plants A. conyzoides L. was carried after careful scrutiny of the area around the department of Microbiology and Molecular Genetics (MMG), University of the Punjab, Lahore. For pre-culture, 2 × 200 ml GYM broth was prepared and pH was adjusted to 7.8. Inoculation was done by adding agar blocks cut from well grown plates. Linear shaking incubation was done at 180 rpm for 3 days at 28 ℃. For crude extract preparation, 2 × 300 m/L of GYM broth for each strain was prepared and the pH was adjusted to 7.8 with 1 N NaOH and 1 N HCl. For each strain duplicate flasks were prepared and two flasks were kept for negative control. The prepared media were autoclaved at 121 ℃ for 20 min. Each flask was inoculated with the 10% pre-culture and incubated at 180 rpm on linear shakers for 3 days at 28 ℃.

Healthy medicinal plants were collected from CSIR-National Botanical Research Institute (Banthara) (26° 55′ N, 80° 59′ E), Lucknow, India during June, 2013. Catechol type and hydroxamate siderophores were estimated by Arnow's method (Arnow 1937) and Csaky test (Csaky 1948) respectively. The amount of IAA produced by Actinomycetes isolates was determined using GYM broth supplemented with different concentration of l-tryptophan (Basal, 0, 2 and 5 mg/ml) and incubated at 30 ℃ with shaking at 125 rev/min for 7 days (Gordon and Weber 1951). Appearance of a pink colour indicated IAA production. The level of IAA produced was estimated by comparison with an IAA standard. Gibberellic acid (GA3) production was determined according to Borrow et al. (1955)(In submerged aerated culture on Raulin-Thom medium (4% sucrose) in 18 days at 25 ℃.).

Healthy medicinal plants were collected from CSIR-National Botanical Research Institute (Banthara) (26° 55′ N, 80° 59′ E), Lucknow, India during June, 2013. Catechol type and hydroxamate siderophores were estimated by Arnow's method (Arnow 1937) and Csaky test (Csaky 1948) respectively. The amount of IAA produced by Actinomycetes isolates was determined using GYM broth supplemented with different concentration of l-tryptophan (Basal, 0, 2 and 5 mg/ml) and incubated at 30 ℃ with shaking at 125 rev/min for 7 days (Gordon and Weber 1951). Appearance of a pink colour indicated IAA production. The level of IAA produced was estimated by comparison with an IAA standard. Gibberellic acid (GA3) production was determined according to Borrow et al. (1955)(In submerged aerated culture on Raulin-Thom medium (4% sucrose) in 18 days at 25 ℃.).

Healthy medicinal plants were collected from CSIR-National Botanical Research Institute (Banthara) (26° 55′ N, 80° 59′ E), Lucknow, India during June, 2013. Catechol type and hydroxamate siderophores were estimated by Arnow's method (Arnow 1937) and Csaky test (Csaky 1948) respectively. The amount of IAA produced by Actinomycetes isolates was determined using GYM broth supplemented with different concentration of l-tryptophan (Basal, 0, 2 and 5 mg/ml) and incubated at 30 ℃ with shaking at 125 rev/min for 7 days (Gordon and Weber 1951). Appearance of a pink colour indicated IAA production. The level of IAA produced was estimated by comparison with an IAA standard. Gibberellic acid (GA3) production was determined according to Borrow et al. (1955)(In submerged aerated culture on Raulin-Thom medium (4% sucrose) in 18 days at 25 ℃.).

Healthy medicinal plants were collected from CSIR-National Botanical Research Institute (Banthara) (26° 55′ N, 80° 59′ E), Lucknow, India during June, 2013. Catechol type and hydroxamate siderophores were estimated by Arnow's method (Arnow 1937) and Csaky test (Csaky 1948) respectively. The amount of IAA produced by Actinomycetes isolates was determined using GYM broth supplemented with different concentration of l-tryptophan (Basal, 0, 2 and 5 mg/ml) and incubated at 30 ℃ with shaking at 125 rev/min for 7 days (Gordon and Weber 1951). Appearance of a pink colour indicated IAA production. The level of IAA produced was estimated by comparison with an IAA standard. Gibberellic acid (GA3) production was determined according to Borrow et al. (1955)(In submerged aerated culture on Raulin-Thom medium (4% sucrose) in 18 days at 25 ℃.).

Healthy medicinal plants were collected from CSIR-National Botanical Research Institute (Banthara) (26° 55′ N, 80° 59′ E), Lucknow, India during June, 2013. Catechol type and hydroxamate siderophores were estimated by Arnow's method (Arnow 1937) and Csaky test (Csaky 1948) respectively. The amount of IAA produced by Actinomycetes isolates was determined using GYM broth supplemented with different concentration of l-tryptophan (Basal, 0, 2 and 5 mg/ml) and incubated at 30 ℃ with shaking at 125 rev/min for 7 days (Gordon and Weber 1951). Appearance of a pink colour indicated IAA production. The level of IAA produced was estimated by comparison with an IAA standard. Gibberellic acid (GA3) production was determined according to Borrow et al. (1955)(In submerged aerated culture on Raulin-Thom medium (4% sucrose) in 18 days at 25 ℃.).

Healthy medicinal plants were collected from CSIR-National Botanical Research Institute (Banthara) (26° 55′ N, 80° 59′ E), Lucknow, India during June, 2013. Catechol type and hydroxamate siderophores were estimated by Arnow's method (Arnow 1937) and Csaky test (Csaky 1948) respectively. The amount of IAA produced by Actinomycetes isolates was determined using GYM broth supplemented with different concentration of l-tryptophan (Basal, 0, 2 and 5 mg/ml) and incubated at 30 ℃ with shaking at 125 rev/min for 7 days (Gordon and Weber 1951). Appearance of a pink colour indicated IAA production. The level of IAA produced was estimated by comparison with an IAA standard. Gibberellic acid (GA3) production was determined according to Borrow et al. (1955)(In submerged aerated culture on Raulin-Thom medium (4% sucrose) in 18 days at 25 ℃.).