Details of Factor

General Information of Factor (ID: FP127)
  Factor Name MID Medium
  Factor Type Environmental Conditions
  Factor Description
Each microbial strain has the potential to produce multiple compounds, but only subsets of these compounds are made under specific growth conditions. Therefore, variations in cultivation parameters can elicit the production and discovery of new secondary metabolites by changing cultivation parameters such as media composition, various nutrients, trace elements, physical parameters (i.e., pH, temperature), and chemical elicitors (i.e., sub-lethal concentrations of antibiotics, communication molecules). Moreover, the co-cultivation of microbes and the addition of factors affecting epigenetic control can also be framed within the OSMAC principle.
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 The Content Variation of Natural Product Induced by This Factor
      Species Name: Pestalotiopsis microspora
          Species Info Click to show the detail information of this Factor
          Experiment Detail
The fresh stem fragments of Taxus wallichiana Zucc., the Himalayan yew, were collected during January 1998 from Shivapuri, 15 km north from Kathmandu at an altitude of 2200 m (voucher specimen KS-100 is deposited at National Herbarium Center, Godawari, Kathmandu). Several fungal endophytes were isolated from its inner bark. Each pure isolate was grown in 500 mL MID media with 1 g soytone (Difco Co) per litre for three weeks
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            Paclitaxel [1]
               Factor Link Part Location NP Content
 
500 mL MID Medium with 1 g soytone (Difco Co) per litre (21Days)
    NP Info    fresh stem Shivapuri, 15 km north from Kathmandu at an altitude of 2200 m (voucher specimen KS-100 is deposited at National Herbarium Center, Godawari, Kathmandu).
NP Content: 25.7 ng/L
 
500 mL MID Medium with 1 g soytone (Difco Co) per litre (21Days)
    NP Info    fresh stem Shivapuri, 15 km north from Kathmandu at an altitude of 2200 m (voucher specimen KS-100 is deposited at National Herbarium Center, Godawari, Kathmandu).
NP Content: 167.2 ng/L
 
500 mL MID Medium with 1 g soytone (Difco Co) per litre (21Days)
    NP Info    fresh stem Shivapuri, 15 km north from Kathmandu at an altitude of 2200 m (voucher specimen KS-100 is deposited at National Herbarium Center, Godawari, Kathmandu).
NP Content: 203.4 ng/L
      Species Name: Sporormia minima
          Species Info Click to show the detail information of this Factor
          Experiment Detail
The fresh stem fragments of Taxus wallichiana Zucc., the Himalayan yew, were collected during January 1998 from Shivapuri, 15 km north from Kathmandu at an altitude of 2200 m (voucher specimen KS-100 is deposited at National Herbarium Center, Godawari, Kathmandu). One of the new isolated fungi, Sporormia minima, was cultured in 2 L MID media containing 1 g soytone per liter for three weeks
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            Paclitaxel [1]
               Factor Link Part Location NP Content
 
500 mL MID Medium with 1 g soytone (Difco Co) per litre (21Days)
    NP Info    fresh stem Shivapuri, 15 km north from Kathmandu at an altitude of 2200 m (voucher specimen KS-100 is deposited at National Herbarium Center, Godawari, Kathmandu).
NP Content: 15.7 ng/L
      Species Name: Trichothecium sp.
          Species Info Click to show the detail information of this Factor
          Experiment Detail
The fresh stem fragments of Taxus wallichiana Zucc., the Himalayan yew, were collected during January 1998 from Shivapuri, 15 km north from Kathmandu at an altitude of 2200 m (voucher specimen KS-100 is deposited at National Herbarium Center, Godawari, Kathmandu). Several fungal endophytes were isolated from its inner bark. Each pure isolate was grown in 500 mL MID media with 1 g soytone (Difco Co) per litre for three weeks
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            Paclitaxel [1]
               Factor Link Part Location NP Content
 
500 mL MID Medium with 1 g soytone (Difco Co) per litre (21Days)
    NP Info    fresh stem Shivapuri, 15 km north from Kathmandu at an altitude of 2200 m (voucher specimen KS-100 is deposited at National Herbarium Center, Godawari, Kathmandu).
NP Content: 165.7 ng/L

References
1 Evidence for paclitaxel from three new endophytic fungi of Himalayan yew of Nepal