| The Content Variation of Natural Product Induced by Different Factor(s) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Species Name: Aspergillus flocculosus strain CBS 112784 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Rice medium | [1] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Fungal material: The endophytic fungus was isolated from the fresh stems of Markhamia platycalyx family (Bignoniaceae) collected in October 2010 from Al-Zohriya gardens (Al-Zamalek, Giza, Egypt). Small-scale fermentation: A small-scale fermentation was carried out in two Erlenmeyer flasks (1 L each) on rice medium, which was prepared with 100 g of rice powder and approximately 100 mL of demineralized water just enough to cover the rice layer. The rice media was autoclaved prior to fungal inoculation. A 15-day fungal inoculum grown on petri dish was inoculated on the sterile rice medium and was allowed to grow at room temperature under static condition for 30 days. Medium-scale 30-day rice culture fermentation: A medium scale fermentation was performed in 10 Erlenmeyer flasks (1 L each) on rice solid medium for 30 days under same condition applied to small scale culture.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Medium-scale fermentation: Rice medium (25℃ + 30 days)
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Fresh stems | Al-Zamalek, Giza, Egypt |
NP Content: 8 mg
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| Species Name: Fusarium solani strain DI16 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: PDA medium | [2] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Isolation of fungal material: The fungus Fusarium solani was isolated from fresh healthy roots of Cassia alata Linn. The plant samples were collected from conservation forest of Gazipur, Bangladesh in August, 2013. Fusarium solani species (internal strain no. CARE-1), which had been isolated following surface sterilization from the root of the plant C. alata were cultivated at 28 ℃ for 28 days on potato dextrose agar medium.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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PDA medium (28℃ + 28 days)
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Fresh healthy roots | Gazipur, Bangladesh |
NP Content: 5.2 mg
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