Endophytic fungus Phomopsis sp. 33# was isolated with PDA medium from the bark of the mangrove plant Rhizophora stylosa, collected in the intertidal region of Zhanjiang, in Guangdong Province, China. Small agar slices bearing mycelia were placed in 1000 mL Erlenmeyer flasks containing rice medium (composed of 60 g rice, 80 mL distilled water, and 0.24 g sea salt) and incubated for 30 days at 28 ℃.