Streptomyces sp. BT01 was isolated from the root tissues of Boesenbergia rotunda (L.) Mansf. A spore suspension of Streptomyces sp. BT01 was prepared in distilled water from cultures grown on ISP-4 medium at 30 ℃ for 10 days. The suspension, 10⁸ spores per 100 ml of liquid medium, was added to ISP-2 broth in each 500-ml Erlenmeyer flask. Cultures were kept on a shaker at 120 rpm at 30 ℃ for 48 h and used as seed stocks. For large production of culture filtrates, the strain BT01 was grown in a modified 3000 ml glass container containing 1500 ml of ISP-2 broth, and incubated in an orbital shaker for 5 days in the same condition.