Strain LgMB259 was isolated by V. divergens leaves collected from the Pantanal, in the region of Nhecol ndia (S18° 10.07′, W57° 23.03′) in Brazil. The Microbispora sp. LGMB259 was cultivated on ISP3-agar plates at 37 ℃ for 7 days. Chunks of agar with the fully-grown strains were used to inoculate five (250 mL) Erlenmeyer flasks, each containing 50 mL of R5A medium . Individual cultures were grown at 37 ℃ for 3 days and subsequently used as seed cultures for the scale-up fermentation. The seed cultures were used to inoculate 80 Erlenmeyer flasks (250 mL) each containing 100 mL of R5A medium. Fermentation (8 L) was continued at 37 ℃ with, shaking (250 rpm) for 10 days.