Details of Factor

General Information of Factor (ID: FP094)
  Factor Name R5A Medium
  Factor Type Environmental Conditions
  Factor Description
Each microbial strain has the potential to produce multiple compounds, but only subsets of these compounds are made under specific growth conditions. Therefore, variations in cultivation parameters can elicit the production and discovery of new secondary metabolites by changing cultivation parameters such as media composition, various nutrients, trace elements, physical parameters (i.e., pH, temperature), and chemical elicitors (i.e., sub-lethal concentrations of antibiotics, communication molecules). Moreover, the co-cultivation of microbes and the addition of factors affecting epigenetic control can also be framed within the OSMAC principle.
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 The Content Variation of Natural Product Induced by This Factor
      Species Name: Microbispora sp. LGMB259
          Species Info Click to show the detail information of this Factor
          Experiment Detail
Strain LgMB259 was isolated by V. divergens leaves collected from the Pantanal, in the region of Nhecol ndia (S18° 10.07′, W57° 23.03′) in Brazil. The Microbispora sp. LGMB259 was cultivated on ISP3-agar plates at 37 ℃ for 7 days. Chunks of agar with the fully-grown strains were used to inoculate five (250 mL) Erlenmeyer flasks, each containing 50 mL of R5A medium . Individual cultures were grown at 37 ℃ for 3 days and subsequently used as seed cultures for the scale-up fermentation. The seed cultures were used to inoculate 80 Erlenmeyer flasks (250 mL) each containing 100 mL of R5A medium. Fermentation (8 L) was continued at 37 ℃ with, shaking (250 rpm) for 10 days.
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            1-(2-Carboxyethyl)-Beta-Carboline-3-Carboxylic Acid 3-Methyl Ester [1]
               Factor Link Part Location NP Content
 
R5A medium (37℃ + 10 days)
    NP Info    Leaves Brazil
NP Content: 11 mg
            1-Vinyl- Beta -Carboline-3-Carboxy Acid [1]
               Factor Link Part Location NP Content
 
R5A medium (37℃ + 10 days)
    NP Info    Leaves Brazil
NP Content: 11 mg
            Indole-3-Acetic Acid [1]
               Factor Link Part Location NP Content
 
R5A medium (37℃ + 10 days)
    NP Info    Leaves Brazil
NP Content: 12.3 mg
            Indole-3-Carboxaldehyde [1]
               Factor Link Part Location NP Content
 
R5A medium (37℃ + 10 days)
    NP Info    Leaves Brazil
NP Content: 10.8 mg
            Indole-3-Carboxylic Acid [1]
               Factor Link Part Location NP Content
 
R5A medium (37℃ + 10 days)
    NP Info    Leaves Brazil
NP Content: 9.6 mg
            Jbir-133 [1]
               Factor Link Part Location NP Content
 
R5A medium (37℃ + 10 days)
    NP Info    Leaves Brazil
NP Content: 11 mg
            Kitasetaline [1]
               Factor Link Part Location NP Content
 
R5A medium (37℃ + 10 days)
    NP Info    Leaves Brazil
NP Content: 40 mg

References
1 Microbispora sp. LGMB259 endophytic actinomycete isolated from Vochysia divergens (Pantanal, Brazil) producing beta-carbolines and indoles with biological activity