| The Content Variation of Natural Product Induced by Different Factor(s) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Species Name: Jishengella endophytica 161111 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Fermentation medium | [1] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Jishengella endophytica 161111 was isolated and identified from the healthy roots of the mangrove plant, Xylocarpus granatum, collected from the mangrove reserve zone in Hainan Province, China. The spores of J. endophytica 161111 were directly cultured in 1000 mL Erlenmeyer flasks containing 200 mL fermentation media consisted of glucose 2%, yeast extract 0.5%, peptone 0.5%, KNO3 1.5%, CaCO3 0.4%, and NaCl 0.4% (pH 7.2). The cultures were incubated on a rotatory shaker at 220 rpm at 28 ℃ for 30 days.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Fermentation medium containing glucose 2%, yeast extract 0.5%, peptone 0.5%, KNO3 1.5%, CaCO3 0.4%, and NaCl 0.4% (28℃ + 30 days + PH7.2 + 220 rpm)
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Roots | Hainan Province, China |
NP Content: 5 mg
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| Species Name: Trichoderma atroviride isolate s361 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Rice medium | [2] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
The strain, No. S361, was isolated from the bark of C. fortunei collected in Jiande, Zhejiang province, China, in May 2009. After cultured on PDA medium in Petri dish for one week, the plaques of S361 mycelium in PDA medium (5 mm in diameter) were punched out and inoculated to 100 Erlenmeyer flasks (1000 mL) using a self-designed cutter. Each shake flask was filled with rice solid medium (250 g rice and 300 mL distilled water), which was subsequently sterillized at 125 ℃ for 20 min. All flasks were incubated at 25 ℃ for 30 days.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Solid rice medium (25℃ +30 days)
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Bark | Jiande, Zhejiang province, China |
NP Content: 13.8 mg
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