The fungus Ampelomyces sp. was isolated from fresh healthy flowers of Urospermum picroides (L.) F.W. Schmidt growing in the wild. The plant was collected in April 2004 near Alexandria, Egypt. Mass growth of the fungus for the isolation and identification of new metabolites was carried out in Erlenmeyer flasks (1 l each). The fungus was grown in liquid Wickerham medium (3 g yeast extract, 3 g malt extract, 5 g peptone, 10 g glucose, distilled water added up to 1000 ml, pH adjusted to 7.2-7.4) or on rice solid medium (to 100 g commercially available rice was added 100 ml of distilled water and kept overnight prior to autoclaving) at room temperature under static conditions for 21 or 30 days, respectively.