The endophytic fungus Aspergillus sp. CPCC 400735 was isolated from the plant Kadsura longipedunculata collected in Leishan of Guizhou province, China. The strain was cultured on PDA (0.3% potato extract, 2% glucose, 1.5% agar power) at 28 ℃ for 5 days. Under aseptic conditions, agar plugs were cut into small pieces and inoculated in 500 mL Erlenmeyer flasks, each containing 100 mL of seed medium [glucose 2%, sucrose 1%, soybean powder 0.2%, peptone 1%, K₂HPO₄ 0.03%, poly(ethylene glycol) 0.25%, NaNO₃ 0.3%, and (NH₄)₂SO₄ 0.3%; the final pH of the medium was adjusted to 6.0 before sterilization with an autoclave at 121 ℃ for 20 min]. Cultures were incubated in flasks at 28 ℃ on a rotary shaker at 190 rpm for 2 days to prepare the seed culture. We filled each of 32 500 mL Erlenmeyer flasks with 80 g of rice and 120 mL of distilled H₂O. After soaking for 5 h, the contents were sterilized with an autoclave at 121 ℃ for 20 min. After cooling to room temperature, each flask was inoculated with 10 mL of seed culture and incubated at 28 ℃ for 40 days.