The endophytic fungus P. minimoides was isolated from selected adult and healthy leaves of H. latiflora, collected by Sol Cristians in Huetamo (18° 31.709′ N, 101° 4.692′ W; 221 masl), State of Michoacan, Mexico, on July 2010. Erlenmeyer flasks with 50 mL of potato-dextrose broth (Difco) sterilized by autoclaving at 121 ℃ for 15 min were individually inoculated with 1 cm³ agar taken from a stock culture of P. minimoides on PDA plates. Cultures grown for 1 week were used to inoculate eight 2.8 L Fernbach flasks containing solid rice medium (200 g of white rice and 400 mL of H₂O). P. minimoides was cultured at room temperature for 45 days in static conditions with a 12/12 h daily light-dark period.