The endophytic fungus Penicillium chermesinum EN-480 was isolated from the marine red alga Pterocladiella tenuis, which was collected at Rongcheng, Shandong Province of P. R. China, in July 2014. The fungal strain P. chermesinum EN-480 was grown on PDA medium at 28 ℃ for 4 days, which was inoculated statically for 30 days in 90 × 1 L Erlenmeyer flasks with rice solid medium at room temperature (each flask contained 70 g of rice, 0.1 g of corn flour, 0.3 g of peptone, 0.1 g of sodium glutamate, and 100 mL of natural, filtered seawater that was obtained from the Huiquan Gulf of the Yellow Sea near the campus of IOCAS, pH 6.5-7.0).