The fungus Phyllosticta capitalensis was isolated from the leaves of Loropetalum chinense var. rubrum, which collected from Nanjing, Jiangsu Province, P. R. China (31° 14′ N, 118° 22′ E), in August 2019. To prepare the seed culture, the strain was cultured on potato dextrose agar (PDA) at 28 &#8451 for 7 days. Agar plugs were cut into small pieces (approximately 0.5 × 0.5 × 0.5 cm³). Small pieces were added to ten Erlenmeyer flasks (250 mL) with 100 mL of potato dextrose liquid medium, and flasks were incubated on a rotary shaker at 28 &#8451 and 150 rpm for 5 days to prepare seed culture. Solid fermentation was carried out in 200 Erlenmeyer flasks (1 L) each containing 140 g of rice and 210 mL of distilled water with 5 mmol/L Nicotinamide, 0.1 mmol/L 5-aza-2-deoxycytidine, 0.25 mmol/L CuSO₄ 5H₂O, 0.5 g/L MgSO₄ and 0.5 g/L NaCl were sterilized at autoclave, and then, 10 mL of seed culture was added into each flask. All flasks were incubated at 28 &#8451 for 30 days.