General Information of Species (ID: SP0246)
  Species Name
Phomopsis sp.
  Species Genus Phomopsis
  Species Family Valsaceae
  Microbe Type Endophytic Fungi
  Studied Organism Phomopsis sp. YM 355364
  External Links
Taxonomy ID 1077315
  Host Plant (s)
Aconitum carmichaeli Species Info

 The Content Variation of Natural Product Induced by Different Factor(s)
      PDB medium [1]
              Factor Info Click to show the detail information of this Factor
               Experiment Detail
Fungal Material. The fungus was isolated from surface-sterilized fresh stems of an apparently healthy Aconitum carmichaeli collected in August 2008 in Huize County, Yunnan Province, P. R. China. The fungal strain was cultured on slants of PDA at 28 ℃ for 7 d. Then, the fresh mycelium was inoculated into 20x500-ml Erlenmeyer flasks each containing 120 ml of PDB medium (200 g potato and 20 g glucose in 1 l of H2O). After 4 d of the incubation at 28 ℃ on a rotary shaker at 200 r.p.m., a 20-ml culture liquid was transferred as seed into each of a total of 100 1-l Erlenmeyer flasks containing 250 ml of PDB medium. The following cultivation was kept for 7 d at 28 ℃ at 200 r.p.m. on a rotary shaker.
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  PDB medium (28℃ +7 days)   (Part: Stems; Location: Huize County, Yunnan Province, China)
               NP Name Link Host Plant Part Location NP Content
 
(5Alpha,6Beta,15Beta,22E)-6-Ethoxy-5,15-Dihydroxyergosta-7,22-Dien-3-One
NP Info Aconitum carmichaeli Stems Huize County, Yunnan Province, China
NP Content: 7.8 mg
 
(14Beta,22E)-9,14-Dihydroxyergosta-4,7,22-Triene-3,6-Dione
NP Info Aconitum carmichaeli Stems Huize County, Yunnan Province, China
NP Content: 10.7 mg
 
Calvasterol B
NP Info Aconitum carmichaeli Stems Huize County, Yunnan Province, China
NP Content: 15.2 mg
 
Ganodermaside D
NP Info Aconitum carmichaeli Stems Huize County, Yunnan Province, China
NP Content: 11.6 mg
 
Calvasterol A
NP Info Aconitum carmichaeli Stems Huize County, Yunnan Province, China
NP Content: 8.5 mg
References
1 Two new steroids from an endophytic fungus Phomopsis sp