Seven endophytic Streptomyces spp. (AImp 6-1, AMbr-1, AFat-1, AB131-1, AB131-2, AB131-3, and DImp6-1) and three Streptomyces spp. isolates obtained from the collections of the Microbiology Laboratory, Bogor Agricultural University (PS4-16, LBR02, and LSW05) were used in this study. Chitinase production was determined using the methods described by Taechowisan et al. An agar disc of each isolate obtained from a 7-day-old culture grown on yeast malt extract medium was placed on a Petri dish containing chitin agar medium (20 g colloidal chitin; 0.1 g K₂HPO₄ ; 0.1 g MgSO₄.7H₂O; 1 g NaCl; 2.5 g(NH₄)2SO₄; 1 g yeast extract; 20 g agar and 1000 ml distilled water). Petri dishes were incubated at 30 ℃ for 6 days. Observations were conducted by measuring the clear zone around the colony (halo) which indicated chitin solubilization by chitinase producing bacteria.

Seven endophytic Streptomyces spp. (AImp 6-1, AMbr-1, AFat-1, AB131-1, AB131-2, AB131-3, and DImp6-1) and three Streptomyces spp. isolates obtained from the collections of the Microbiology Laboratory, Bogor Agricultural University (PS4-16, LBR02, and LSW05) were used in this study. Chitinase production was determined using the methods described by Taechowisan et al. An agar disc of each isolate obtained from a 7-day-old culture grown on yeast malt extract medium was placed on a Petri dish containing chitin agar medium (20 g colloidal chitin; 0.1 g K₂HPO₄ ; 0.1 g MgSO₄.7H₂O; 1 g NaCl; 2.5 g(NH₄)2SO₄; 1 g yeast extract; 20 g agar and 1000 ml distilled water). Petri dishes were incubated at 30 ℃ for 6 days. Observations were conducted by measuring the clear zone around the colony (halo) which indicated chitin solubilization by chitinase producing bacteria.

Seven endophytic Streptomyces spp. (AImp 6-1, AMbr-1, AFat-1, AB131-1, AB131-2, AB131-3, and DImp6-1) and three Streptomyces spp. isolates obtained from the collections of the Microbiology Laboratory, Bogor Agricultural University (PS4-16, LBR02, and LSW05) were used in this study. Chitinase production was determined using the methods described by Taechowisan et al. An agar disc of each isolate obtained from a 7-day-old culture grown on yeast malt extract medium was placed on a Petri dish containing chitin agar medium (20 g colloidal chitin; 0.1 g K₂HPO₄ ; 0.1 g MgSO₄.7H₂O; 1 g NaCl; 2.5 g(NH₄)2SO₄; 1 g yeast extract; 20 g agar and 1000 ml distilled water). Petri dishes were incubated at 30 ℃ for 6 days. Observations were conducted by measuring the clear zone around the colony (halo) which indicated chitin solubilization by chitinase producing bacteria.

Seven endophytic Streptomyces spp. (AImp 6-1, AMbr-1, AFat-1, AB131-1, AB131-2, AB131-3, and DImp6-1) and three Streptomyces spp. isolates obtained from the collections of the Microbiology Laboratory, Bogor Agricultural University (PS4-16, LBR02, and LSW05) were used in this study. Chitinase production was determined using the methods described by Taechowisan et al. An agar disc of each isolate obtained from a 7-day-old culture grown on yeast malt extract medium was placed on a Petri dish containing chitin agar medium (20 g colloidal chitin; 0.1 g K₂HPO₄ ; 0.1 g MgSO₄.7H₂O; 1 g NaCl; 2.5 g(NH₄)2SO₄; 1 g yeast extract; 20 g agar and 1000 ml distilled water). Petri dishes were incubated at 30 ℃ for 6 days. Observations were conducted by measuring the clear zone around the colony (halo) which indicated chitin solubilization by chitinase producing bacteria.

Seven endophytic Streptomyces spp. (AImp 6-1, AMbr-1, AFat-1, AB131-1, AB131-2, AB131-3, and DImp6-1) and three Streptomyces spp. isolates obtained from the collections of the Microbiology Laboratory, Bogor Agricultural University (PS4-16, LBR02, and LSW05) were used in this study. Chitinase production was determined using the methods described by Taechowisan et al. An agar disc of each isolate obtained from a 7-day-old culture grown on yeast malt extract medium was placed on a Petri dish containing chitin agar medium (20 g colloidal chitin; 0.1 g K₂HPO₄ ; 0.1 g MgSO₄.7H₂O; 1 g NaCl; 2.5 g(NH₄)2SO₄; 1 g yeast extract; 20 g agar and 1000 ml distilled water). Petri dishes were incubated at 30 ℃ for 6 days. Observations were conducted by measuring the clear zone around the colony (halo) which indicated chitin solubilization by chitinase producing bacteria.

Seven endophytic Streptomyces spp. (AImp 6-1, AMbr-1, AFat-1, AB131-1, AB131-2, AB131-3, and DImp6-1) and three Streptomyces spp. isolates obtained from the collections of the Microbiology Laboratory, Bogor Agricultural University (PS4-16, LBR02, and LSW05) were used in this study. Chitinase production was determined using the methods described by Taechowisan et al. An agar disc of each isolate obtained from a 7-day-old culture grown on yeast malt extract medium was placed on a Petri dish containing chitin agar medium (20 g colloidal chitin; 0.1 g K₂HPO₄ ; 0.1 g MgSO₄.7H₂O; 1 g NaCl; 2.5 g(NH₄)2SO₄; 1 g yeast extract; 20 g agar and 1000 ml distilled water). Petri dishes were incubated at 30 ℃ for 6 days. Observations were conducted by measuring the clear zone around the colony (halo) which indicated chitin solubilization by chitinase producing bacteria.

Seven endophytic Streptomyces spp. (AImp 6-1, AMbr-1, AFat-1, AB131-1, AB131-2, AB131-3, and DImp6-1) and three Streptomyces spp. isolates obtained from the collections of the Microbiology Laboratory, Bogor Agricultural University (PS4-16, LBR02, and LSW05) were used in this study. Chitinase production was determined using the methods described by Taechowisan et al. An agar disc of each isolate obtained from a 7-day-old culture grown on yeast malt extract medium was placed on a Petri dish containing chitin agar medium (20 g colloidal chitin; 0.1 g K₂HPO₄ ; 0.1 g MgSO₄.7H₂O; 1 g NaCl; 2.5 g(NH₄)2SO₄; 1 g yeast extract; 20 g agar and 1000 ml distilled water). Petri dishes were incubated at 30 ℃ for 6 days. Observations were conducted by measuring the clear zone around the colony (halo) which indicated chitin solubilization by chitinase producing bacteria.

Seven endophytic Streptomyces spp. (AImp 6-1, AMbr-1, AFat-1, AB131-1, AB131-2, AB131-3, and DImp6-1) and three Streptomyces spp. isolates obtained from the collections of the Microbiology Laboratory, Bogor Agricultural University (PS4-16, LBR02, and LSW05) were used in this study. Chitinase production was determined using the methods described by Taechowisan et al. An agar disc of each isolate obtained from a 7-day-old culture grown on yeast malt extract medium was placed on a Petri dish containing chitin agar medium (20 g colloidal chitin; 0.1 g K₂HPO₄ ; 0.1 g MgSO₄.7H₂O; 1 g NaCl; 2.5 g(NH₄)2SO₄; 1 g yeast extract; 20 g agar and 1000 ml distilled water). Petri dishes were incubated at 30 ℃ for 6 days. Observations were conducted by measuring the clear zone around the colony (halo) which indicated chitin solubilization by chitinase producing bacteria.