| The Content Variation of Natural Product Induced by Different Factor(s) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Chitin agar medium | [1] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Factor Info
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| Experiment Detail |
Seven endophytic Streptomyces spp. (AImp 6-1, AMbr-1, AFat-1, AB131-1, AB131-2, AB131-3, and DImp6-1) and three Streptomyces spp. isolates obtained from the collections of the Microbiology Laboratory, Bogor Agricultural University (PS4-16, LBR02, and LSW05) were used in this study. Chitinase production was determined using the methods described by Taechowisan et al. An agar disc of each isolate obtained from a 7-day-old culture grown on yeast malt extract medium was placed on a Petri dish containing chitin agar medium (20 g colloidal chitin; 0.1 g K2HPO4 ; 0.1 g MgSO4.7H2O; 1 g NaCl; 2.5 g(NH4)2SO4; 1 g yeast extract; 20 g agar and 1000 ml distilled water). Petri dishes were incubated at 30 ℃ for 6 days. Observations were conducted by measuring the clear zone around the colony (halo) which indicated chitin solubilization by chitinase producing bacteria.
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| Chitin agar medium (30℃ + 6 days) (Part: .; Location: .) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| NP Name | Link | Host | Plant Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Chitinase
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NP Info | . | NA | NA |
NP Content: 21 mm
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| References | ||||||||||||||||||||||||
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| 1 | Endophytic Streptomyces spp. as Biocontrol Agents of Rice Bacterial Leaf Blight Pathogen (Xanthomonas oryzae pv. oryzae) | |||||||||||||||||||||||
