| The Content Variation of Natural Product Induced by Different Factor(s) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Species Name: Artemisia absinthium | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Chemotype Comparison | [1] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Ten different plants of wormwood were collected in March 1997 from each one of the following four wild populations in the Spanish Pyrenees: Tallo de Aulet (prov. Huesca) and Pont de Suert, Sort and Farga de Moles (prov. Lleida). In three of the four populations studied, there was another chemotype, with 25-65% of cis-epoxyocimene and 15-50% of chrysanthenyl acetate. This chemotype, called chemotype B, was less frequent in the Pyrenees than the chemotype A, appearing only in 17% of the samples (two samples in TallO de Aulet and in Pont de Suert and three samples in Farga de Moles).
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| Factor Function |
Two chemotypes were detected; a cis-epoxyocimene type (with more than 50% of this compound) which was predominant in all the populations, and a cis-epoxyocimene + chrysanthenyl acetate type (with 25-65% of cis-epoxyocimene and 15-50% of chrysanthenyl acetate). The distribution of these chemotypes had no relation with the altitude of the samples.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Chemotype (cis-epoxyocimene type)
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Leaves | Spain |
NP Content: <0.03 %
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Chemotype (cis-epoxyocimene + chrysanthenyl acetate type)
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Leaves | Spain |
NP Content: 0.08 %
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| Species Name: Citrus sinensis (Hongjiang) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Variety Comparison | [2] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Four kinds of fresh sweet oranges were obtained in the same season, November 2000, in Guangzhou. Citrus sinensis var. Hongjiang (called 'hong jiang chen' in Chinese) and C. sinensis Osbeck var. Anliu (called 'luo gang chen') were obtained at an orchard in Luo gang in Guangzhou (25 km from the center of Guangzhou). Citrus sinensis var. Sihui (called 'sihui ju') was harvested at the Shigou Experimental Farm in Sihui City in Guangdong Province (75 km far away from Guangzhou). Citrus sinensis var. Washington navel (called 'qi chen') which was produced in Jiangxi Province (200 km from Guangzhou; bordering Guangdong Province), was purchased at the wholesale market in Guangzhou. All oranges were kept in a cold room until prepared a few days later.
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| Factor Function |
The peel oil compositions of four kinds of sweet oranges in China, Citrus sinensis Osbeck var. Hongjian, C. sinensis Osbeck var. Anliu, C. sinensis Osbeck var. Sihui and C. sinensis Osbeck var. Washington navel, were investigated by GC and GC/MS. The essential oils were extracted by cold-pressing method. Forty-two to 53 compounds were quantitatively determined for each variety. Their percentages, respectively, were: > 97.3%, > 98.4%, > 97.5% and > 98.0% in hydrocarbons; > 1.5%, > 0.7%, > 0.8% and > 0.9% in total aldehydes; 0.8%, 0.5%, 0.5% and 0.5% in alcohols. Either cis-or trans-limonene oxide was detected in small amounts in each of the four samples, with Hongjiang containing both limonene oxides. delta-3-Carene was commonly quantified at a level of 0.1% in all the samples. The content of aliphatic aldehydes, including octanal, nonanal, decanal and dodecanal, exceeded that of terpene aldehydes, such as neral and geranial in Hongjiang (0.9%) and Washington navel (0.6%), whereas the aliphatic aldehydes in Anliu and Sihui were present to a lesser degree than the terpene aldehydes. Either alpha- or beta-sinensal was detected in trace amounts in each of the four samples. Linalool was the major alcohol in all the samples. Nootkatone was not detected.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Citrus sinensis var. Anliu
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Fruits | China |
NP Content: < 0.005 %
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Citrus sinensis var. Hongjiang
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Fruits | China |
NP Content: < 0.005 %
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Citrus sinensis var. Sihui
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Fruits | China |
NP Content: < 0.005 %
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Citrus sinensis var. Washington navel
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Fruits | China |
NP Content: < 0.005 %
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| Species Name: Coriandrum sativum | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Locality Variation | [3] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Two samples (20 kg each) of mature coriander (Coriandrum sativum L.) fruits were used for this study. The first was purchased from a spice market of Korba in Tunisia (Tn), the second, from Canada (Can), was supplied by General Herboristerie Laboratory (Marseille, France).
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| Factor Function |
The first from Tunisia (Tn) and the second from Canada (Can). The highest essential oil yield was observed for Can with 0.44% (w/w) and 0.37% (w/w) for Tn. Forty-five compounds were identified in the essential oils and the main compound of both samples was linalool. The total phenol contents varied between two coriander fruit samples; Can sample presented high polyphenol contents (15.16 mg GAE/g) compared with Tn one (12.10 mg GAE/g). Significant differences were also found in total tannin contents among representing 0.7 mg GAE/g in Can and 0.34 mg GAE/g in Tn. The highest contents of total flavonoids were observed in Can sample with 13.2 mg CE/g.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Locality: Canada
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Fruits | Canada |
NP Content: 0.1 %
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| Species Name: Cymbopogon martinii | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Ethyl methane sulfonate Treatment; Ethyleneimine Treatment; Gamma ray Treatment; Cultivar Comparison | [4] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
The materials (dry palmarosa seeds with 12% moisture content of four cultivars 'Tripta', 'Trishna', 'PRC' and 'MP') and methods of gamma rays (15 Kr, 60CO source with dose rate 0.38 Mrads\h) and chemicals ethyl methane sulfonate (EMS - 0.4%) and ethyleneimine (EI-0.04%) induced mutagenesis. The seed of the four varieties had already been selfed for 4-5 flowering seasons before mutagenesis to maintain genetic homogeneity (or purity) and thereafter all the selected M1 generation suspected mutant plants were individually selfed by bagging to give rise to controlled M2 plant to progeny segregants for further selection. Oil content was estimated on freshly harvested herbage (stems, leaves and inflorescence) using a Clevenger-type apparatus.
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| Factor Function |
The oil content was increased in all the 20 mutants as compared to their respective contols. Most M2 generation mutants were found to exhibit a straight relationship between high herbage (stem, leaves and inflorescence) yield, oil content (%) and oil quality in terms of major and trace constituents of the oil. Six mutants specifically were endowed with the desirable rosy note which remained predominant in the samples of Trishna-gamma-5, MP-gamma-13, Tripta-gamma-8, Tripta-Ethyl methane sulfonate (EMS)-19, Tripta-EMS-21 and PRC-Ethyleneimine (EI)-44. The fresh herbage and oil yield and odor criteria, i.e., rosy note were satisified by the three best mutants, viz., Trishna-gamma-5, MP-gamma-13 and Tripta-gamma-19. The results have been interpreted in the sense that induction of mutations brings about gene level changes from dominance to recessive and vice versa in morpho-economic traits having quantitative trait loci (QTL) under polygenic genetic control.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Cymbopogon martinii cv. MP (Control)
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Herbages | India |
NP Content: 0.09 %
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Mutant 31: C. martinii cv. MP induced by ethyl methane sulfonate
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Herbages | India |
NP Content: 0.1 %
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Mutant 31: C. martinii cv. MP induced by ethyl methane sulfonate
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Herbages | India |
NP Content: 0.1 %
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Mutant 13: C. martinii cv. MP induced by gamma rays
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Herbages | India |
NP Content: 0.1 %
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Cymbopogon martinii cv. PRC (Control)
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Herbages | India |
NP Content: 0.07 %
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Mutant 43: C. martinii cv. PRC induced by ethyleneimine
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Herbages | India |
NP Content: 0.08 %
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Mutant 44: C. martinii cv. PRC induced by ethyleneimine
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Herbages | India |
NP Content: 0.88 %
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Mutant 45: C. martinii cv. PRC induced by ethyleneimine
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Herbages | India |
NP Content: 0.07 %
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Mutant 48: C. martinii cv. PRC induced by ethyleneimine
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Herbages | India |
NP Content: 0.1 %
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Mutant 9: C. martinii cv. PRC induced by gamma rays
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Herbages | India |
NP Content: 0.1 %
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Mutant 10: C. martinii cv. PRC induced by gamma rays
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Herbages | India |
NP Content: 0.11 %
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Mutant 12: C. martinii cv. PRC induced by gamma rays
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Herbages | India |
NP Content: 0.04 %
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Cymbopogon martinii cv. Tripta (Control)
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Herbages | India |
NP Content: 0.1 %
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Mutant 18: C. martinii cv. Tripta induced by ethyl methane sulfonate
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Herbages | India |
NP Content: 0.11 %
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Mutant 19: C. martinii cv. Tripta induced by ethyl methane sulfonate
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Herbages | India |
NP Content: 0.13 %
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Mutant 20: C. martinii cv. Tripta induced by ethyl methane sulfonate
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Herbages | India |
NP Content: 0.11 %
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Mutant 21: C. martinii cv. Tripta induced by ethyl methane sulfonate
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Herbages | India |
NP Content: 0.05 %
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Mutant 1: C. martinii cv. Trishna induced by gamma rays
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Herbages | India |
NP Content: 0.1 %
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Mutant 2: C. martinii cv. Trishna induced by gamma rays
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Herbages | India |
NP Content: 0.1 %
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Cymbopogon martinii cv. Trishna (Control)
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Herbages | India |
NP Content: 0.09 %
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Mutant 41: C. martinii cv. Trishna induced by ethyleneimine
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Herbages | India |
NP Content: 0.06 %
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Mutant 5: C. martinii cv. Trishna induced by gamma rays
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Herbages | India |
NP Content: 0.12 %
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Mutant 6: C. martinii cv. Trishna induced by gamma rays
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Herbages | India |
NP Content: 0.12 %
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Mutant 8: C. martinii cv. Trishna induced by gamma rays
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Herbages | India |
NP Content: 0.1 %
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| Species Name: Melaleuca ericifolia | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Locality Variation | [5] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Samples of M. ericifolia leaves were obtained from 19 locations as follows: DL3104- 3110, Coopernook, New South Wales (NSW), 31° 49′ 31″ S, 152° 36′ 48″ E (Site No. 1); DL3114-3120, Hawks Nest, NSW, 32° 40′ 09″ S, 152° 10′ 12″ E (Site No. 2); DL3240-3244, Hexham, NSW, 32° 48′ 50″ S, 151° 42′ E (Site No. 3); DL3245-3249, The Entrance, NSW, 32° 22′ 24″ S, 151° 28′ 19″ E (Site No. 4); DL3397-3401, Tuggerah Lake, NSW, 33° 21′ S, 151° 27′ E (Site No. 5); DL3250-3254, Georges River, NSW, 33° 58′ 42″ S, 151° 00′ 14″ E (Site No. 6); DL3255-3259, Berry, NSW, 34° 46′ 37″ S, 150° 45′ 27″ E (Site No. 7); DL3260-3264, Lake Durras, NSW, 35° 36′ 00″ S, 150° 16′ 17″ E (Site No. 8); DL3265- 3269, Wallaga Lake, NSW, 36° 23′ 43″ S, 150° 03′ 04″ E (Site No. 9); DL3270-3274, Wallagoot, NSW, 36° 44′ 50″ S, 149° 55′ 46″ E (Site No. 10); DL3275-3279, Genoa, Victoria (Vic), 37° 25′ 56″ S, 149° 38′ 41″ E (Site No. 11); BVG3024- 3028, West of Lakes Entrance, Vic, 37° 48′ S, 148° 03′E (Site No. 12); BVG3014-3018, West of Lang Lang, Vic, 38° 13′ S, 145° 30′ 13″ E (Site No. 13); BVG3019-3023, East of Welshpool, Vic, 38° 38′ 28″ S, 146° 30′53″ E (Site No. 14); ACC1019/1-2, 5-7, Nelson on the Glenelg River, Vic, 38° 03′ S, 141° 00′ E (Site No. 15); KJ1-5, Airport Flinders Island, Tasmania (Tas), 40° 05′ S, 148° 00′ E (Site No. 16); KJ6-10, Lackrana Road Flinders Island, Tas, 40° 18′ S, 148° 06′ E (Site No. 17); ACR1848/1-3, Woolnorth Point, Tas, 40° 38′ 30″ S, 144° 43′ 30″ E (Site No. 18); JB4509, Robins Island Track, Tas, 40° 45′ S, 144°53′E (Site No. 19). The majority of samples were collected during June to December 1999 with the exceptions being sites 5, 15 and 18, which were collected during July to October 2000. Leaf material totaling about 100 g of fresh leaves and twigs was obtained mainly from five widely spaced individual trees per location.
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| Factor Function |
Oil composition varied quantitatively throughout the species range rather than qualitatively in an apparent association with latitude of occurrence. Linalool and linalool oxide were abundant in the oils from the north of the species range in New South Wales with a gradual southerly decline in these compounds to central Victoria with concomitant increase in the proportions of 1,8-cineole, alpha-terpineol and limonene. The most southerly populations sampled in southern Victoria and Tasmania gave oils containing relatively high proportions of 1,8-cineole (mean 34.5%) and low proportions of linalool (3%). Four populations from the Central Coast of NSW (Coopernook, Hawks Nest, The Entrance and Tuggerah Lake) provided the greatest opportunity of identifying seed trees that combine the attributes required for plantation development. The tree that had the best combination of oil traits (DL 3116 from Hawks Nest) had an oil yield of 4.5%, a linalool content of 60% and a 1,8-cineole content of 16%.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Locality: northern Australia
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Leaves | Australia |
NP Content: 0.3 %
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Locality: southern Australia
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Leaves | Australia |
NP Content: 0.3 %
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| Species Name: Myrtus communis var. italica | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Month Variation | [6] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Myrtle (M. communis var. italica) aerial parts were collected monthly during 2006-2007 from Jbal Stara of Haouaria region in North Tunisia, belonging to a subhumid bioclimate.
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| Factor Function |
In conclusion, high fluctuations were observed in the oil yields and composition of different parts of Myrtus communis var. italica during all the collecting periods. They could be explained by genetic and environmental factors. Moreover, significant differences were revealed in the main oil compounds. alpha-Pinene percentages showed the most remarkable changes among the different part oils. So, leaf oils contained more alpha-pinene than those of the fruits and stems during the myrtle vegetative cycle.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Leaf: (Harvesting time: January)
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Leaves | Tunisia |
NP Content: 0.1 %
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Leaf: (Harvesting time: February)
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Leaves | Tunisia |
NP Content: 0.1 %
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Leaf: (Harvesting time: March)
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Leaves | Tunisia |
NP Content: 0.1 %
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Leaf: (Harvesting time: April)
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Leaves | Tunisia |
NP Content: 0.1 %
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Leaf: (Harvesting time: May)
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Leaves | Tunisia |
NP Content: 0.2 %
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Leaf: (Harvesting time: June)
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Leaves | Tunisia |
NP Content: 0.7 %
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Leaf: (Harvesting time: July)
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Leaves | Tunisia |
NP Content: 0.1 %
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Leaf: (Harvesting time: August)
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Leaves | Tunisia |
NP Content: 0.1 %
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Leaf: (Harvesting time: September)
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Leaves | Tunisia |
NP Content: 0.1 %
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Leaf: (Harvesting time: October)
|
Leaves | Tunisia |
NP Content: 0.1 %
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Leaf: (Harvesting time: November)
|
Leaves | Tunisia |
NP Content: 0.1 %
|
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Leaf: (Harvesting time: December)
|
Leaves | Tunisia |
NP Content: 0.1 %
|
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Fruit: (Harvesting time: January)
|
Fruits | Tunisia |
NP Content: 0.1 %
|
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Fruit: (Harvesting time: August)
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Fruits | Tunisia |
NP Content: 6.1 %
|
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Fruit: (Harvesting time: September)
|
Fruits | Tunisia |
NP Content: 0.1 %
|
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Fruit: (Harvesting time: October)
|
Fruits | Tunisia |
NP Content: 0.1 %
|
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Fruit: (Harvesting time: November)
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Fruits | Tunisia |
NP Content: 0.1 %
|
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Fruit: (Harvesting time: December)
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Fruits | Tunisia |
NP Content: 0.1 %
|
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Stem: (Harvesting time: January)
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Stems | Tunisia |
NP Content: 0.5 %
|
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Stem: (Harvesting time: February)
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Stems | Tunisia |
NP Content: 0.6 %
|
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Stem: (Harvesting time: March)
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Stems | Tunisia |
NP Content: 0.6 %
|
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Stem: (Harvesting time: April)
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Stems | Tunisia |
NP Content: 1.7 %
|
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Stem: (Harvesting time: May)
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Stems | Tunisia |
NP Content: 0.9 %
|
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Stem: (Harvesting time: June)
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Stems | Tunisia |
NP Content: 0.1 %
|
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Stem: (Harvesting time: July)
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Stems | Tunisia |
NP Content: 0.2 %
|
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Stem: (Harvesting time: August)
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Stems | Tunisia |
NP Content: 0.1 %
|
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Stem: (Harvesting time: September)
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Stems | Tunisia |
NP Content: 0.1 %
|
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Stem: (Harvesting time: October)
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Stems | Tunisia |
NP Content: 0.6 %
|
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Stem: (Harvesting time: November)
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Stems | Tunisia |
NP Content: 0.5 %
|
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Stem: (Harvesting time: December)
|
Stems | Tunisia |
NP Content: 0.4 %
|
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| Species Name: Ocimum basilicum | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Variety Comparison | [7] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Aerial parts of Ocimum basilicum var. purpurascens Benth, Ocimum basilicum var. dianatnejadii Salimi at flowering stage were collected from plants grown in Experimental Station of Pykan Shahr, near Tehran. Elevation 1215 m above sea level, latitude 35° 42′ North, 51° 8′ East, average humidity 36% and climatic category semi-arid.
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| Factor Function |
Methyl chavicol (43.0%) and linalool (28.9%) were identified as the major compounds in the oil of O. basilicum var. purpurascens, while methyl chavicol (37.6%), linalool (33.4%) and alpha-cadinol (5.7%) were the major constituents in the oil of O. basilicum var. dianatnejadii.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Ocimum basilicum var. dianatnejadii Salimi
|
Aerial parts | Iran |
NP Content: 0.3 %
|
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| Species Name: Ocimum basilicum L | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Chemotype Comparison | [8] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
The study was conducted in North-Central Anatolia under semi arid conditions. Seeds of 18 basil landraces (O. basilicum L.) were collected from local farms and home gardens in Turkey. To examine essential oil composition of the basil landraces without environmental influences, the plants were grown under identical (same environmental and soil conditions) conditions. Seeds were sown on a medium (1:1:1 washed sand, horse manure and field soil) in greenhouse conditions on March 25, 2003. Seedlings were grown until the 3-5 leaf stage. The seedlings were transplanted into pilots in the Gaziosmanpasxa University Experimental Research Station on May 15, 2003. The plants were harvested at the full blooming stage and dried at 35 ℃ for essential oil isolation.
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| Factor Function |
Variation of essential oils in the landraces was subjected to cluster analysis, and seven different chemotypes were identified. They were (1) linalool, (2) methyl cinnamate, (3) methyl cinnamate/linalool, (4) methyl eugenol, (5) citral, (6) methyl chavicol (estragol), and (7) methyl chavicol/citral. Methyl chavicol with high citral contents (methyl chavicol/citral) can be considered as a 'new chemotype' in the Turkish basils.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
Chemotype (linalool-rich type)
|
Leaves | Turkey |
NP Content: 0.1 %
|
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|
Chemotype (citral-rich type)
|
Leaves | Turkey |
NP Content: 1.4 %
|
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|
Chemotype (methyl chavicol and citral-rich type)
|
Leaves | Turkey |
NP Content: 1.8 %
|
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| Species Name: Pelargonium graveolens L'Her. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Cultivar Comparison; Seasonal Variation | [9] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
One-month-old rooted stem cuttings of three rosescented geranium cultivars, namely Bourbon type, CIM-Pawan and Kelkar were transplanted at 50 × 50 cm plant spacing in the experimental field of Central Institute of Medicinal and Aromatic Plants, Research Center Purara, Uttarakhand, in October 2007. Plants were irrigated immediately after transplanting and further crops were raised following the normal agricultural practices needed to grow the plant. The experimental site is located between the coordinates 28° 60′ and 31° 29′ N, 77° 49′ and 80° 60 m E, and at an altitude of 1250 m in the Kattyur valley. Climatologically, it is categorized as a sub-temperate (1200-1700 m) zone, where monsoon usually breaks in June and continues up to September. Sampling of the rose-scented geranium cultivars was started from March 2008 and taken on the tenth of every month until February 2009. Samples were collected in triplicate in each season. (A, cv. Bourbon type; B, cv. CIM-Pawan; C, cv. Kelkar; I, spring season; II, summer season; III, rainy season;IV, autumn season; V, winter season).
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| Factor Function |
The major components in the essential oil of cv. Bourbon type were geraniol (14.1-34.6%), citronellol (15.2-31.3%), linalool (2.9-9.2%), citronellyl formate (4.4-9.2%), isomenthone (4.5-6.6%), 10-epi-gamma-eudesmol (4.7-6.7%) and geranyl formate (3.8-6.2%). The dominant constituents of the cv. CIM-Pawan essential oil were geraniol (11.9-31.9%), citronellol (16.1-30.2%), citronellyl formate (5.2-8.9%), linalool (3.7-6.4%), isomenthone (4.0-6.3%), 10-epi-gamma-eudesmol (4.4-5.2%) and geranyl formate (4.3-5.0%). However, the chemical composition and odor of cv. Kelkar was quite different from the other two cultivars and the major components found in this oil were citronellol (51.0-63.4%) and isomenthone (9.8-17.8%).
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Pelargonium graveolens cv. Bourbon type: (Harvesting time: spring season)
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Fresh herb | Iran |
NP Content: 0.8 %
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Pelargonium graveolens cv. Bourbon type: (Harvesting time: summer season)
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Fresh herb | Iran |
NP Content: 1.1 %
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Pelargonium graveolens cv. Bourbon type: (Harvesting time: autumn season)
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Fresh herb | Iran |
NP Content: 1.5 %
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Pelargonium graveolens cv. Bourbon type: (Harvesting time: winter season)
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Fresh herb | Iran |
NP Content: 0.3 %
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Pelargonium graveolens cv. Bourbon type: (Harvesting time: rainy season)
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Fresh herb | Iran |
NP Content: 1.2 %
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Pelargonium graveolens cv. CIM-Pawan: (Harvesting time: spring season)
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Fresh herb | Iran |
NP Content: 0.7 %
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Pelargonium graveolens cv. CIM-Pawan: (Harvesting time: summer season)
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Fresh herb | Iran |
NP Content: 1 %
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Pelargonium graveolens cv. CIM-Pawan: (Harvesting time: autumn season)
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Fresh herb | Iran |
NP Content: 1.6 %
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Pelargonium graveolens cv. CIM-Pawan: (Harvesting time: winter season)
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Fresh herb | Iran |
NP Content: 0.6 %
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Pelargonium graveolens cv. CIM-Pawan: (Harvesting time: rainy season)
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Fresh herb | Iran |
NP Content: 1.1 %
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Pelargonium graveolens cv. Kelkar: (Harvesting time: spring season)
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Fresh herb | Iran |
NP Content: 0.3 %
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Pelargonium graveolens cv. Kelkar: (Harvesting time: summer season)
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Fresh herb | Iran |
NP Content: 0.2 %
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| Species Name: Pulicaria dysenterica | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Locality Variation | [10] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Aerial parts of P. dysenterica were collected during the flowering stage from two different locations in Greece in August 2002. Sample A: Katara (Perfecture Trikala). Sample B: Arahova (Perfecture Viotia).
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| Factor Function |
Fifty-four components were identified representing 80.5% (sample A) and 72.6% (sample B) of the total oils. The main components in sample A were (Z)-nerolidol (11.2%), caryophyllene oxide (9.1%) and (E)-nerolidol (6.6%), while those of sample B were beta-caryophyllene (12.8%), caryophyllene oxide (12.8%) and (E)-nerolidol (6.9%).
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Locality: Katara, Perfecture Trikala, Greece
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Aerial parts | Greece |
NP Content: 6.2 %
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Locality: Arahova, Perfecture Viotia, Greece
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Aerial parts | Greece |
NP Content: 4 %
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| Species Name: Salvia sclarea | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Locality Variation | [11] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
200 g of fresh flowering spikes were collected randomly at full bloom stage (browning of lower floret stage) from the 2006-2007 crops of clary sage cultivar CIM-Chandni cultivated at CIMAP Lucknow and resource center Purara, Uttarakhand. The oil of Kashmir origin was collected from the Chemistry division of IIIM Jammu.
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| Factor Function |
Linalool (23.6%), alpha-terpineol (3.8%), linalyl acetate (51.2%), beta-caryophyllene (3.2%), germacrene D (1.3%) and sclareol (1.3%) were recorded in the oil S. sclarea cultivated in Lucknow UP while the Kashmir oil sample possessed the highest percentage of linalyl acetate (60.8%) and lowest linalool (14.5%) along with alpha-terpineol (1.8%), geranyl acetate (2.2%), beta-caryophyllene (1.9%), germacrene D (2.6%) and sclareol (1.3%) as the other minor constituents. In contrast, the oil of S. sclarea from Purara in Uttarakhand showed highest percentage of linalool (29.8%), alpha-terpineol (5.3%) and sclareol (2.3%) and the lowest linalyl acetate (45.7%) among all the three samples.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Locality: Lucknow UP, India
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Spikes | India |
NP Content: 0.7 %
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Locality: Jammu and Kashmir, India
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Spikes | India |
NP Content: 0.4 %
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Locality: Uttarakhand, India
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Spikes | India |
NP Content: 0.9 %
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| Species Name: Satureja parnassica ssp. parnassica | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Month Variation; Developmental Stage Variation | [12] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Fresh plant materials were obtained in 2004 and 2005. S. thymbra 1(vegetative stage: just before flowering, date: June 7, 2004, location: Mt. Immitos, altitude(m): 350); S. thymbra 2(vegetative stage: full flowering, date: July 7, 2004, location: Mt. Immitos, altitude(m): 350); S. thymbra 3(vegetative stage: after flowering, date: Aug 7, 2004, location: Mt. Immitos, altitude(m): 350); S. thymbra 4(vegetative stage: fruiting, date: Sept 7, 2004, location: Mt. Immitos, altitude(m): 350); S. thymbra 5(vegetative stage: fruiting, date: Nov 7, 2004, location: Mt. Immitos, altitude(m): 350); S. thymbra 6(vegetative stage: fruiting, date: Feb 7, 2005, location: Mt. Immitos, altitude(m): 350); S. thymbra 7(vegetative stage: before flowering, date: May 7, 2005, location: Mt. Immitos, altitude(m): 350); S. parnassica 8(vegetative stage: before flowering, date: June 16, 2004, location: Mt. Parnon, altitude(m): 1800); S. parnassica 9(vegetative stage: just before flowering, date: July 16, 2004, location: Mt. Parnon, altitude(m): 1800); S. parnassica 10(vegetative stage: full flowering, date: Aug 16, 2004, location: Mt. Parnon, altitude(m): 1800); S. parnassica 11(vegetative stage: after flowering, date: Sept 16, 2004, location: Mt. Parnon, altitude(m): 1800).
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| Factor Function |
It is evident that the phytochemical content of the essential oils for both Satureja species varied greatly, depending on the period examined, and showed large prevalence of phenolic content. It must also be pointed out that regardless of the vegetative stage of the plant collected, the sum of the two isomeric phenol monoterpenes (carvacrol and thymol) and their biosynthetic monoterpene precursors p-cymene and gamma-terpinene represented always the bulk of each essential oil (~76%). More specificallysfor both species-during their premature vegetative stage, gamma-terpinene constitutes the major component of their essential oils. The approach of the flowering period results in the simultaneous gradual diminishment of monoterpene precursors and the prevalence of their phenolic metabolites. Thus, essential oils obtained from plants collected during the 'just before their flowering' stage contain thymol as their major component, which constitutes 27.88 and 38.51% of the total oil content for S. thymbra and S. parnassica, respectively. On the other hand, during their full flowering period carvacrol prevails as the major component, accounting for 39.10% for S. thymbra and for 34.61% for S. parnassica. The end of the flowering stage delineates a sharp decrease of carvacrol levels and the predominance of thymol as the major component of the essential oils. A few months later, as the premature vegetative stage approached, the level of gamma-terpinene was restored. The content of p-cymenesthe other major monoterpene precursor-fluctuated seasonally in a manner similar to that shown by gamma-terpinene. Other monoterpene hydrocarbons such as myrcene and alpha-terpinene were also detected in smaller quantities, whereas various monoterpene alcohols such as linalool, borneol, and terpin-4-ol were found mainly in the oils obtained after the flowering stage. Finally, it is notable that the oils obtained during the just before the full flowering period contain beta-caryophyllene as one of their major components.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Harvesting time: before flowering satge; 16-June-2004
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Leaves and stems | Mt. Parnon, Peloponnese |
NP Content: 0.14 %
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Harvesting time: just before flowering satge; 16-June-2004
|
Leaves and stems | Mt. Parnon, Peloponnese |
NP Content: 0.31 %
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| Species Name: Satureja thymbra | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Month Variation; Developmental Stage Variation | [12] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Fresh plant materials were obtained in 2004 and 2005. S. thymbra 1(vegetative stage: just before flowering, date: June 7, 2004, location: Mt. Immitos, altitude(m): 350); S. thymbra 2(vegetative stage: full flowering, date: July 7, 2004, location: Mt. Immitos, altitude(m): 350); S. thymbra 3(vegetative stage: after flowering, date: Aug 7, 2004, location: Mt. Immitos, altitude(m): 350); S. thymbra 4(vegetative stage: fruiting, date: Sept 7, 2004, location: Mt. Immitos, altitude(m): 350); S. thymbra 5(vegetative stage: fruiting, date: Nov 7, 2004, location: Mt. Immitos, altitude(m): 350); S. thymbra 6(vegetative stage: fruiting, date: Feb 7, 2005, location: Mt. Immitos, altitude(m): 350); S. thymbra 7(vegetative stage: before flowering, date: May 7, 2005, location: Mt. Immitos, altitude(m): 350); S. parnassica 8(vegetative stage: before flowering, date: June 16, 2004, location: Mt. Parnon, altitude(m): 1800); S. parnassica 9(vegetative stage: just before flowering, date: July 16, 2004, location: Mt. Parnon, altitude(m): 1800); S. parnassica 10(vegetative stage: full flowering, date: Aug 16, 2004, location: Mt. Parnon, altitude(m): 1800); S. parnassica 11(vegetative stage: after flowering, date: Sept 16, 2004, location: Mt. Parnon, altitude(m): 1800).
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| Factor Function |
It is evident that the phytochemical content of the essential oils for both Satureja species varied greatly, depending on the period examined, and showed large prevalence of phenolic content. It must also be pointed out that regardless of the vegetative stage of the plant collected, the sum of the two isomeric phenol monoterpenes (carvacrol and thymol) and their biosynthetic monoterpene precursors p-cymene and gamma-terpinene represented always the bulk of each essential oil (~76%). More specificallysfor both species-during their premature vegetative stage, gamma-terpinene constitutes the major component of their essential oils. The approach of the flowering period results in the simultaneous gradual diminishment of monoterpene precursors and the prevalence of their phenolic metabolites. Thus, essential oils obtained from plants collected during the 'just before their flowering' stage contain thymol as their major component, which constitutes 27.88 and 38.51% of the total oil content for S. thymbra and S. parnassica, respectively. On the other hand, during their full flowering period carvacrol prevails as the major component, accounting for 39.10% for S. thymbra and for 34.61% for S. parnassica. The end of the flowering stage delineates a sharp decrease of carvacrol levels and the predominance of thymol as the major component of the essential oils. A few months later, as the premature vegetative stage approached, the level of gamma-terpinene was restored. The content of p-cymenesthe other major monoterpene precursor-fluctuated seasonally in a manner similar to that shown by gamma-terpinene. Other monoterpene hydrocarbons such as myrcene and alpha-terpinene were also detected in smaller quantities, whereas various monoterpene alcohols such as linalool, borneol, and terpin-4-ol were found mainly in the oils obtained after the flowering stage. Finally, it is notable that the oils obtained during the just before the full flowering period contain beta-caryophyllene as one of their major components.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Harvesting time: full flowering satge; 7-June-2004
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Leaves, stems and flowers | Mt. Immitos, Continental Greece |
NP Content: 0.31 %
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Harvesting time: after flowering satge; 7-August-2004
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Leaves, stems and flowers | Mt. Immitos, Continental Greece |
NP Content: <0.05 %
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Harvesting time: fruiting satge; 7-November-2004
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Leaves and stems | Mt. Immitos, Continental Greece |
NP Content: 0.42 %
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Harvesting time: fruiting satge; 7-February- 2005
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Leaves and stems | Mt. Immitos, Continental Greece |
NP Content: 0.34 %
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Harvesting time: before flowering satge; 7-May-2005
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Leaves and stems | Mt. Immitos, Continental Greece |
NP Content: 0.09 %
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Harvesting time: full flowering satge; 16-August-2004
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Leaves, stems and flowers | Mt. Parnon, Peloponnese |
NP Content: <0.05 %
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| Species Name: Teucrium chamaedrys | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Locality Variation | [13] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
The aerial parts of T. chamaedrys were collected at the flowering stage in June 2004 near Corti, Corsica, France and near Oristano, Sardinia, Italy
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| Factor Function |
The Corsican and Sardinian oils of T. chamaedrys investigated in this study were qualitatively similar but they differed by the amount of their major components. The major components were beta-caryophyllene (29.0% and 27.4%, respectively) and germacrene D (19.4% and 13.5%, respectively), followed by alpha-humulene (6.8%) and delta-cadinene (5.4%) in the Corsican oil and by caryophyllene oxide (12.3%) and alpha-humulene (6.5%) in the Sardinian oil. These quantitative differences are also noticeable on the amounts of the different class compounds. Especially, the monoterpene hydrocarbons amounted for 10.3% and 4.1% in Sardinian and Corsican oils respectively and the oxygenated sesquiterpenes amounted for 18.9% and only 7.4% in both oils, respectively. Both oils were qualitatively rather similar in comparison with those reported in the literature from various geographic regions. However, among the 87 components identified in this study, 47 minor components (< 0.6%) reported were identified for the first time in T. chamaedrys oil. This study confirms the quantitative variability of the major components according to the plant origin.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Locality: Corti, Corsica, France
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Aerial parts | France |
NP Content: 0.1 %
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Locality: Oristano, Sardinia, Italy
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Aerial parts | Italy |
NP Content: <0.05 %
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| Species Name: Thymus leucostomus | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Altitude Variation | [14] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Herbal parts were collected from A = Eskisehir: Suluagac village in Turkey, altitude 1100 m, in July 1990 and B = Corum: Osmancik, Berk village in Turkey, altitude 580-600 m, on 22 June 1993.
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| Factor Function |
One chemotype (Suluagac village, Eskisehir, Turkey) contained carvacrol (21.59%), p-cymene (17.80%) and thymol (14.10%); and the other chemotype (Berk village, Corum, Turkey) contained alpha-terpinyl acetate (23.80%), borneol (12.85%), linalool (13.67%) and thymol (11.31%) as major constituents.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Locality: Berk village, Corum, Turkey; Altitude 580-600 m
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Herbal parts | Turkey |
NP Content: 0.03 %
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| Species Name: Thymus longicaulis | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Chemotype Comparison | [15] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Aerial parts of the plants with distinct odors, harvested at full flowering stage, were collected from the same population (growing in an area of one m2) on Mt. Parnis Attiki, at an altitude of 1200 m in June 1995.
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| Factor Function |
Limonene (18.7%) and thymol (19.4%); geraniol (56.8%) and geranyl acetate (7.6%); linalool (63.1%) and alpha-terpinyl acetate (20.4%) were the predominant components in each of the three different chemotypes, respectively.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Chemotype (thyme-odor type)
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Aerial parts | Attiki, Greece |
NP Content: 4 %
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Chemotype (rose-odor type)
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Aerial parts | Attiki, Greece |
NP Content: 6.3 %
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| Species Name: Thymus pseudopulegioides | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Locality Variation | [16] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Plant materials were collected from the following localities in north western Turkey. A = Trabzon: Caykara, Soganli dag on July 28, 1994; B = Bayburt: Caykara, Mohakambo yaylasi on July 25, 1994; C = Trabzon: Koprubasi, Vizara yaylasi on July 20, 1994.
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| Factor Function |
One hundred and four compounds were identified representing 97.5-99.5% of the total components detected in thymol/carvacrol (50.14/10.67%), thymol/linalool (23.14/20.24%) and linalool/alpha-terpinyl acetate/geraniol (21.55/16.70/11.17%) rich oils.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Locality: Soganli dag, Caykara, Trabzon, Eskisehir, Turkey
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Aerial parts | Eskisehir, Turkey |
NP Content: 0.1 %
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Locality: Vizara yaylasi, Koprubasi, Trabzon, Eskisehir, Turkey
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Aerial parts | Eskisehir, Turkey |
NP Content: 0.6 %
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| Species Name: Thymus vulgaris | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Cultivar Comparison; Seasonal Variation | [9] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
One-month-old rooted stem cuttings of three rosescented geranium cultivars, namely Bourbon type, CIM-Pawan and Kelkar were transplanted at 50 × 50 cm plant spacing in the experimental field of Central Institute of Medicinal and Aromatic Plants, Research Center Purara, Uttarakhand, in October 2007. Plants were irrigated immediately after transplanting and further crops were raised following the normal agricultural practices needed to grow the plant. The experimental site is located between the coordinates 28° 60′ and 31° 29′ N, 77° 49′ and 80° 60 m E, and at an altitude of 1250 m in the Kattyur valley. Climatologically, it is categorized as a sub-temperate (1200-1700 m) zone, where monsoon usually breaks in June and continues up to September. Sampling of the rose-scented geranium cultivars was started from March 2008 and taken on the tenth of every month until February 2009. Samples were collected in triplicate in each season. (A, cv. Bourbon type; B, cv. CIM-Pawan; C, cv. Kelkar; I, spring season; II, summer season; III, rainy season;IV, autumn season; V, winter season).
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| Factor Function |
The major components in the essential oil of cv. Bourbon type were geraniol (14.1-34.6%), citronellol (15.2-31.3%), linalool (2.9-9.2%), citronellyl formate (4.4-9.2%), isomenthone (4.5-6.6%), 10-epi-gamma-eudesmol (4.7-6.7%) and geranyl formate (3.8-6.2%). The dominant constituents of the cv. CIM-Pawan essential oil were geraniol (11.9-31.9%), citronellol (16.1-30.2%), citronellyl formate (5.2-8.9%), linalool (3.7-6.4%), isomenthone (4.0-6.3%), 10-epi-gamma-eudesmol (4.4-5.2%) and geranyl formate (4.3-5.0%). However, the chemical composition and odor of cv. Kelkar was quite different from the other two cultivars and the major components found in this oil were citronellol (51.0-63.4%) and isomenthone (9.8-17.8%).
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Pelargonium graveolens cv. Kelkar: (Harvesting time: winter season)
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Fresh herb | Iran |
NP Content: 0.7 %
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Pelargonium graveolens cv. Kelkar: (Harvesting time: rainy season)
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Fresh herb | Iran |
NP Content: 0.1 %
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