| The Content Variation of Natural Product Induced by Different Factor(s) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Species Name: Aspergillus flocculosus strain CBS 112784 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Rice medium | [1] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Fungal material: The endophytic fungus was isolated from the fresh stems of Markhamia platycalyx family (Bignoniaceae) collected in October 2010 from Al-Zohriya gardens (Al-Zamalek, Giza, Egypt). Small-scale fermentation: A small-scale fermentation was carried out in two Erlenmeyer flasks (1 L each) on rice medium, which was prepared with 100 g of rice powder and approximately 100 mL of demineralized water just enough to cover the rice layer. The rice media was autoclaved prior to fungal inoculation. A 15-day fungal inoculum grown on petri dish was inoculated on the sterile rice medium and was allowed to grow at room temperature under static condition for 30 days. Medium-scale 30-day rice culture fermentation: A medium scale fermentation was performed in 10 Erlenmeyer flasks (1 L each) on rice solid medium for 30 days under same condition applied to small scale culture.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Medium-scale fermentation: Rice medium (25℃ + 30 days)
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Fresh stems | Al-Zamalek, Giza, Egypt |
NP Content: 7 mg
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| Species Name: Botryosphaeria dothidea strain KJ-1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Rice Medium | [2] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
The endophytic fungal strain KJ-1 was isolated from the symptomless tissue of stem bark of M. azedarach L., which was collected at Yangling, Shaanxi province, China, in August 2011. The producing strain was cultured on a plate of potato dextrose agar (PDA) medium at 28 ± 0.5 °C for 5 days. Then one piece (approximately 7 mm2) of mycelium was inoculated aseptically to 50 mL Erlenmeyer flasks each containing 20 mL of PD liquid medium, and the seed liquids were incubated at 28 ± 0.5 °C for 3 days on a rotary shaker at 120 rpm. A suspension (100 µL) of the seed liquid was inoculated aseptically to 500 mL Erlenmeyer flasks each containing 60 g of rice and 90 mL of distilled water. There were 300 flasks in total, and the flask cultures were incubated at 28 ± 0.5 °C for 4 weeks.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Rice Medium (28 ± 0.5 degrees Celsius + 28Days)
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tissue of stem bark | Yangling, Shaanxi province, China |
NP Content: 6.5 mg
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