General Information of Species (ID: SP0660)
  Species Name
Botryosphaeria dothidea
  Species Genus Botryosphaeria
  Species Family Botryosphaeriaceae
  Microbe Type Endophytic Fungi
  Studied Organism Botryosphaeria dothidea strain KJ-1
  External Links
Taxonomy ID 55169
  Host Plant (s)
Melia azedarach Species Info

 The Content Variation of Natural Product Induced by Different Factor(s)
      Rice Medium [1]
              Factor Info Click to show the detail information of this Factor
               Experiment Detail
The endophytic fungal strain KJ-1 was isolated from the symptomless tissue of stem bark of M. azedarach L., which was collected at Yangling, Shaanxi province, China, in August 2011. The producing strain was cultured on a plate of potato dextrose agar (PDA) medium at 28 ± 0.5 °C for 5 days. Then one piece (approximately 7 mm2) of mycelium was inoculated aseptically to 50 mL Erlenmeyer flasks each containing 20 mL of PD liquid medium, and the seed liquids were incubated at 28 ± 0.5 °C for 3 days on a rotary shaker at 120 rpm. A suspension (100 µL) of the seed liquid was inoculated aseptically to 500 mL Erlenmeyer flasks each containing 60 g of rice and 90 mL of distilled water. There were 300 flasks in total, and the flask cultures were incubated at 28 ± 0.5 °C for 4 weeks.
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  Rice Medium (28 ± 0.5 degrees Celsius + 28Days)   (Part: tissue of stem bark; Location: Yangling, Shaanxi province, China)
               NP Name Link Host Plant Part Location NP Content
 
3-Methoxy-4-hydroxy-6-methylpyridine-2(1H)-one
NP Info Melia azedarach tissue of stem bark Yangling, Shaanxi province, China
NP Content: 8 mg
 
5-(hydroxymethyl)-1H-pyrrole-2-carbaldehyde
NP Info Melia azedarach tissue of stem bark Yangling, Shaanxi province, China
NP Content: 2.5 mg
 
5-Hydroxymethylfurfural
NP Info Melia azedarach tissue of stem bark Yangling, Shaanxi province, China
NP Content: 5 mg
 
Cerebroside C
NP Info Melia azedarach tissue of stem bark Yangling, Shaanxi province, China
NP Content: 85 mg
 
Sitogluside
NP Info Melia azedarach tissue of stem bark Yangling, Shaanxi province, China
NP Content: 40 mg
 
Chaetoglobosin F
NP Info Melia azedarach tissue of stem bark Yangling, Shaanxi province, China
NP Content: 13 mg
 
(1R,7Z,9S,11Z,13R,14S,16R,17S,18R,19S)-19-(1H-indol-3-ylmethyl)-7,9,16,17-tetramethyl-15-oxa-20-azatetracyclo[11.8.0.01,18.014,16]henicosa-7,11-diene-2,5,6,21-tetrone
NP Info Melia azedarach tissue of stem bark Yangling, Shaanxi province, China
NP Content: 13 mg
 
Ergosterol Peroxide
NP Info Melia azedarach tissue of stem bark Yangling, Shaanxi province, China
NP Content: 6.5 mg
 
Pycnophorin
NP Info Melia azedarach tissue of stem bark Yangling, Shaanxi province, China
NP Content: 25 mg
 
Stemphyperylenol
NP Info Melia azedarach tissue of stem bark Yangling, Shaanxi province, China
NP Content: 13 mg
 
Altenuene
NP Info Melia azedarach tissue of stem bark Yangling, Shaanxi province, China
NP Content: 12 mg
 
Altenusin
NP Info Melia azedarach tissue of stem bark Yangling, Shaanxi province, China
NP Content: 38 mg
 
Alternariol monomethyl ether
NP Info Melia azedarach tissue of stem bark Yangling, Shaanxi province, China
NP Content: 8.5 mg
 
Alternariol
NP Info Melia azedarach tissue of stem bark Yangling, Shaanxi province, China
NP Content: 9.5 mg
 
3-hydroxy-N-(1-hydroxy-3-methylpentan-2-yl)-5-oxohexanamide
NP Info Melia azedarach tissue of stem bark Yangling, Shaanxi province, China
NP Content: 9 mg
 
N-(1-(Hydroxymethyl)-3-methylbutyl)-3-hydroxy-5-oxohexanamide
NP Info Melia azedarach tissue of stem bark Yangling, Shaanxi province, China
NP Content: 9 mg
 
5'-Methoxy-6-Methylbiphenyl-3,4,3'-Triol
NP Info Melia azedarach tissue of stem bark Yangling, Shaanxi province, China
NP Content: 90 mg
 
7-Hydroxy-1-Isochromanone
NP Info Melia azedarach tissue of stem bark Yangling, Shaanxi province, China
NP Content: 2.5 mg
References
1 Secondary metabolites from the endophytic Botryosphaeria dothidea of Melia azedarach and their antifungal, antibacterial, antioxidant, and cytotoxic activities