General Information of Natural Product (ID: NP1399)
  Natural Product Name
Bulgariol

 The Content Variation of Natural Product Induced by Different Factor(s)
      Species Name: Bulgaria inquinans isolate MSp3-1
  Factor Name: Czapek medium [1]
              Species Info Factor Info
               Experiment Detail
Fungal material and fermentation: The fungus Bulgaria inquinans (isolate MSp3-1) was isolated from a healthy sprout of mistletoe (Viscum album), collected in January 2017 at Julich, Germany. The fungus was cultivated on solid Czapek medium, which was prepared by autoclaving 200 mL of liquid Czapek medium with the addition of 5.0 g of bacto agar in a 1 L Erlenmeyer flask. The composition of liquid Czapek medium was 10.0 g dextrose, 20.0 g mannitol, 20.0 g maltose, 3.0 g yeast extract, 1.0 g corn steep liquor, 0.5 g tryptophan, 0.5 g K2HPO4.3H2O, 0.3 g MgSO4.7H2O and 1 L of distilled water (pH value of the medium adjusted between 7.2-7.8). The fermentation was performed in 15 flasks at room temperature, under static conditions for 27 days. OSMAC experiments were carried out by growing the fungus on solid Czapek medium containing either 3.5 g NaCl, 3.5 g NaBr, 3.5 g NaI, 3.5 g NaNO3, 3.5 g (NH4)2SO4 or mixtures of (a) MgSO4.7H2O, NaNO3 and NaCl (2.5 g of each), (b) FeSO4.7H2O, NaNO3 and NaCl (2.5 g of each), or (c) ZnSO4.7H2O, NaNO3 and NaCl (2.5 g of each), added to each 1 L flask followed by extraction when the flasks were completely overgrown by the fungus. Based on the chromatographic profiles obtained from these experiments, a large-scale fermentation of B. inquinans was carried out by adding a mixture of MgSO4.7H2O, NaNO3 and NaCl (2.5 g of each) to solid Czapek medium. The fungus was grown under static conditions for 33 days followed by extraction.
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               Factor Part Location NP Content
 
Solid Czapek medium (25℃ + 27 days)
Sprouts Julich, Germany
NP Content: 2.2 mg
References
1 Expanding the chemical diversity of an endophytic fungus Bulgaria inquinans, an ascomycete associated with mistletoe, through an OSMAC approach