General Information of Species (ID: SP0201)
  Species Name
Bulgaria inquinans
  Species Genus Bulgaria
  Species Family Phacidiaceae
  Microbe Type Endophytic Fungi
  Studied Organism Bulgaria inquinans isolate MSp3-1
  External Links
Taxonomy ID 73172
Genbank ID MK246763
  Host Plant (s)
Viscum album Species Info

 The Content Variation of Natural Product Induced by Different Factor(s)
      Czapek medium [1]
              Factor Info Click to show the detail information of this Factor
               Experiment Detail
Fungal material and fermentation: The fungus Bulgaria inquinans (isolate MSp3-1) was isolated from a healthy sprout of mistletoe (Viscum album), collected in January 2017 at Julich, Germany. The fungus was cultivated on solid Czapek medium, which was prepared by autoclaving 200 mL of liquid Czapek medium with the addition of 5.0 g of bacto agar in a 1 L Erlenmeyer flask. The composition of liquid Czapek medium was 10.0 g dextrose, 20.0 g mannitol, 20.0 g maltose, 3.0 g yeast extract, 1.0 g corn steep liquor, 0.5 g tryptophan, 0.5 g K2HPO4.3H2O, 0.3 g MgSO4.7H2O and 1 L of distilled water (pH value of the medium adjusted between 7.2-7.8). The fermentation was performed in 15 flasks at room temperature, under static conditions for 27 days. OSMAC experiments were carried out by growing the fungus on solid Czapek medium containing either 3.5 g NaCl, 3.5 g NaBr, 3.5 g NaI, 3.5 g NaNO3, 3.5 g (NH4)2SO4 or mixtures of (a) MgSO4.7H2O, NaNO3 and NaCl (2.5 g of each), (b) FeSO4.7H2O, NaNO3 and NaCl (2.5 g of each), or (c) ZnSO4.7H2O, NaNO3 and NaCl (2.5 g of each), added to each 1 L flask followed by extraction when the flasks were completely overgrown by the fungus. Based on the chromatographic profiles obtained from these experiments, a large-scale fermentation of B. inquinans was carried out by adding a mixture of MgSO4.7H2O, NaNO3 and NaCl (2.5 g of each) to solid Czapek medium. The fungus was grown under static conditions for 33 days followed by extraction.
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  Solid Czapek medium (25℃ + 27 days)   (Part: Sprouts; Location: Julich, Germany)
               NP Name Link Host Plant Part Location NP Content
 
Bulgarialactone B
NP Info Viscum album Sprouts Julich, Germany
NP Content: 27 mg
 
Xenofuranone B
NP Info Viscum album Sprouts Julich, Germany
NP Content: 1.2 mg
 
(5Z)-3-Hydroxy-4-Phenyl-5-(Phenylmethylene)-2(5H)-Furanone
NP Info Viscum album Sprouts Julich, Germany
NP Content: 1.9 mg
 
Bulgariline A
NP Info Viscum album Sprouts Julich, Germany
NP Content: 20 mg
 
Bulgariline B
NP Info Viscum album Sprouts Julich, Germany
NP Content: 2.8 mg
 
Bulgariline C
NP Info Viscum album Sprouts Julich, Germany
NP Content: 1.5 mg
 
Bulgariol
NP Info Viscum album Sprouts Julich, Germany
NP Content: 2.2 mg
 
Phenylbutyrolactone IIa
NP Info Viscum album Sprouts Julich, Germany
NP Content: 124 mg
  Solid Czapek medium with the salt mixture (33 days)   (Part: Sprouts; Location: Julich, Germany)
               NP Name Link Host Plant Part Location NP Content
 
Bulgarialactone D
NP Info Viscum album Sprouts Julich, Germany
NP Content: 4 mg
 
(-)-(S)-Flavipesin B
NP Info Viscum album Sprouts Julich, Germany
NP Content: 6.5 mg
 
(5Z)-3-Hydroxy-4-Phenyl-5-(Phenylmethylene)-2(5H)-Furanone
NP Info Viscum album Sprouts Julich, Germany
NP Content: 2.8 mg
 
Bulgariapyrone A
NP Info Viscum album Sprouts Julich, Germany
NP Content: 2.6 mg
 
Bulgariapyrone B
NP Info Viscum album Sprouts Julich, Germany
NP Content: 9.1 mg
 
Bulgariapyrone C
NP Info Viscum album Sprouts Julich, Germany
NP Content: 2.6 mg
 
Bulgariapyrone D
NP Info Viscum album Sprouts Julich, Germany
NP Content: 30.4 mg
 
Bulgariapyrone E
NP Info Viscum album Sprouts Julich, Germany
NP Content: 9.3 mg
 
Bulgariline C
NP Info Viscum album Sprouts Julich, Germany
NP Content: 14.6 mg
 
Bulgariline D
NP Info Viscum album Sprouts Julich, Germany
NP Content: 3.2 mg
 
Bulgarixine A
NP Info Viscum album Sprouts Julich, Germany
NP Content: 2 mg
 
Bulgarixine B
NP Info Viscum album Sprouts Julich, Germany
NP Content: 1.5 mg
References
1 Expanding the chemical diversity of an endophytic fungus Bulgaria inquinans, an ascomycete associated with mistletoe, through an OSMAC approach