General Information of Species (ID: SP0260)
  Species Name
Streptomyces sp.
  Species Genus Streptomyces
  Species Family Streptomycetaceae
  Microbe Type Endophytic Actinomycetes
  Studied Organism Streptomyces sp. R-5
  External Links
Taxonomy ID 1931
  Host Plant (s)
Rhododendron Species Info

 The Content Variation of Natural Product Induced by Different Factor(s)
      Liquid medium [1]
              Factor Info Click to show the detail information of this Factor
               Experiment Detail
Endophytic actinomycetes were isolated from field-grown rhododendron plants for selecting candidates for biological agents to control plant diseases. A spore suspension of R5 (1 ml) was inoculated into a 300-ml flask containing 100 ml of seed medium composed of 1g soluble starch, 0.5g glucose, 0.3g NZ-case, 0.2g yeast extract, 0.5g tryptone, 0.1g K2HPO4, 0.05g MgSO4.7H2O, and 0.3g CaCO3 (pH 7.0). The seed culture was incubated for 3 days at 30 ℃ on a rotary shaker (200rpm). A 3-ml aliquot of the culture was added to each of 12 flasks containing 100 ml of production medium composed of 0.5g glucose, 2g glycerol, 2g soluble starch, 1.5g Pharmamedia (Traders Protein, Memphis, TN, USA), 0.3g yeast extract, and 0.1g Diaion HP-20 resin (Mitsubishi Chemicals, Tokyo, Japan); it was then incubated on a rotary shaker (200rpm) at 30 ℃ for 6 days.
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  Liquid medium containing 0.5g glucose, 2g glycerol, 2g soluble starch, 1.5g Pharmamedia, 0.3g yeast extract, and 0.1g Diaion HP-20 resin (30℃ + 6 days + 200 rpm)   (Part: .; Location: .)
               NP Name Link Host Plant Part Location NP Content
 
1-Pentene
NP Info Rhododendron NA NA
NP Content: 3.5 mg
 
Actinomycin
NP Info Rhododendron NA NA
NP Content: 15.2 mg
References
1 Identification of endophytic Streptomyces sp. R-5 and analysis of its antimicrobial metabolites