| The Content Variation of Natural Product Induced by Different Factor(s) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Species Name: Actinomadura glauciflava strain S4215 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Peptone water | [1] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Healthy leaf, shoot and root tissues of Eaglewood (Aquilaria crassna Pierre ex Lec.) were collected from the plantations in Nakhonnayok province (14° 7′ 20.60″ N, 101° 4′ 16.23″ E) and Phetchabun province (16° 25′ 27.59″ N, 101° 9′ 42.25″ E) of Thailand, during the period of September-October 2008. The actinomycetes isolates were grown on HT agar at 30 ℃ for 3 weeks. Four-millimeter-diameter agar plugs were cut with a sterile cork borer from the leading edges of colonies, and one such plug was transferred into a 250 ml Erlenmeyer flask containing 100 ml of ISP-2 broth and incubated at 30 ℃ for 3 weeks for preparation of seed culture. One percent of seed culture was propagated in ISP-2 broth supplemented with 0.2% L-tryptophan and incubated in the dark at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in peptone water and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in modified Gaus No.1 broth and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. Catechol-type siderophores were determined by using Arnow's method (Arnow 1937) and hydroxamate siderophores were determined by using the Csaky test (Csaky 1984).
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Peptone water (30℃ + 21 days)
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Healthy leaves, shoots and root tissues | Nakhonnayok province and Phetchabun province, Thailand |
NP Content: 7 mg/ml
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| Species Name: Kibdelosporangium sp. S4312 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Peptone water | [1] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Healthy leaf, shoot and root tissues of Eaglewood (Aquilaria crassna Pierre ex Lec.) were collected from the plantations in Nakhonnayok province (14° 7′ 20.60″ N, 101° 4′ 16.23″ E) and Phetchabun province (16° 25′ 27.59″ N, 101° 9′ 42.25″ E) of Thailand, during the period of September-October 2008. The actinomycetes isolates were grown on HT agar at 30 ℃ for 3 weeks. Four-millimeter-diameter agar plugs were cut with a sterile cork borer from the leading edges of colonies, and one such plug was transferred into a 250 ml Erlenmeyer flask containing 100 ml of ISP-2 broth and incubated at 30 ℃ for 3 weeks for preparation of seed culture. One percent of seed culture was propagated in ISP-2 broth supplemented with 0.2% L-tryptophan and incubated in the dark at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in peptone water and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in modified Gaus No.1 broth and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. Catechol-type siderophores were determined by using Arnow's method (Arnow 1937) and hydroxamate siderophores were determined by using the Csaky test (Csaky 1984).
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Peptone water (30℃ + 21 days)
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Healthy leaves, shoots and root tissues | Nakhonnayok province and Phetchabun province, Thailand |
NP Content: 2 mg/ml
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| Species Name: Nocardia alba strain S4301 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Peptone water | [1] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Healthy leaf, shoot and root tissues of Eaglewood (Aquilaria crassna Pierre ex Lec.) were collected from the plantations in Nakhonnayok province (14° 7′ 20.60″ N, 101° 4′ 16.23″ E) and Phetchabun province (16° 25′ 27.59″ N, 101° 9′ 42.25″ E) of Thailand, during the period of September-October 2008. The actinomycetes isolates were grown on HT agar at 30 ℃ for 3 weeks. Four-millimeter-diameter agar plugs were cut with a sterile cork borer from the leading edges of colonies, and one such plug was transferred into a 250 ml Erlenmeyer flask containing 100 ml of ISP-2 broth and incubated at 30 ℃ for 3 weeks for preparation of seed culture. One percent of seed culture was propagated in ISP-2 broth supplemented with 0.2% L-tryptophan and incubated in the dark at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in peptone water and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in modified Gaus No.1 broth and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. Catechol-type siderophores were determined by using Arnow's method (Arnow 1937) and hydroxamate siderophores were determined by using the Csaky test (Csaky 1984).
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Peptone water (30℃ + 21 days)
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Healthy leaves, shoots and root tissues | Nakhonnayok province and Phetchabun province, Thailand |
NP Content: 20 mg/ml
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| Species Name: Nocardia jiangxiensis strain S3308 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Peptone water | [1] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Healthy leaf, shoot and root tissues of Eaglewood (Aquilaria crassna Pierre ex Lec.) were collected from the plantations in Nakhonnayok province (14° 7′ 20.60″ N, 101° 4′ 16.23″ E) and Phetchabun province (16° 25′ 27.59″ N, 101° 9′ 42.25″ E) of Thailand, during the period of September-October 2008. The actinomycetes isolates were grown on HT agar at 30 ℃ for 3 weeks. Four-millimeter-diameter agar plugs were cut with a sterile cork borer from the leading edges of colonies, and one such plug was transferred into a 250 ml Erlenmeyer flask containing 100 ml of ISP-2 broth and incubated at 30 ℃ for 3 weeks for preparation of seed culture. One percent of seed culture was propagated in ISP-2 broth supplemented with 0.2% L-tryptophan and incubated in the dark at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in peptone water and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in modified Gaus No.1 broth and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. Catechol-type siderophores were determined by using Arnow's method (Arnow 1937) and hydroxamate siderophores were determined by using the Csaky test (Csaky 1984).
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Peptone water (30℃ + 21 days)
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Healthy leaves, shoots and root tissues | Nakhonnayok province and Phetchabun province, Thailand |
NP Content: 30 mg/ml
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| Species Name: Nocardia jiangxiensis strain S3311 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Peptone water | [1] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Healthy leaf, shoot and root tissues of Eaglewood (Aquilaria crassna Pierre ex Lec.) were collected from the plantations in Nakhonnayok province (14° 7′ 20.60″ N, 101° 4′ 16.23″ E) and Phetchabun province (16° 25′ 27.59″ N, 101° 9′ 42.25″ E) of Thailand, during the period of September-October 2008. The actinomycetes isolates were grown on HT agar at 30 ℃ for 3 weeks. Four-millimeter-diameter agar plugs were cut with a sterile cork borer from the leading edges of colonies, and one such plug was transferred into a 250 ml Erlenmeyer flask containing 100 ml of ISP-2 broth and incubated at 30 ℃ for 3 weeks for preparation of seed culture. One percent of seed culture was propagated in ISP-2 broth supplemented with 0.2% L-tryptophan and incubated in the dark at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in peptone water and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in modified Gaus No.1 broth and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. Catechol-type siderophores were determined by using Arnow's method (Arnow 1937) and hydroxamate siderophores were determined by using the Csaky test (Csaky 1984).
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Peptone water (30℃ + 21 days)
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Healthy leaves, shoots and root tissues | Nakhonnayok province and Phetchabun province, Thailand |
NP Content: 25 mg/ml
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| Species Name: Nonomuraea rubra strain S3304 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Peptone water | [1] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Healthy leaf, shoot and root tissues of Eaglewood (Aquilaria crassna Pierre ex Lec.) were collected from the plantations in Nakhonnayok province (14° 7′ 20.60″ N, 101° 4′ 16.23″ E) and Phetchabun province (16° 25′ 27.59″ N, 101° 9′ 42.25″ E) of Thailand, during the period of September-October 2008. The actinomycetes isolates were grown on HT agar at 30 ℃ for 3 weeks. Four-millimeter-diameter agar plugs were cut with a sterile cork borer from the leading edges of colonies, and one such plug was transferred into a 250 ml Erlenmeyer flask containing 100 ml of ISP-2 broth and incubated at 30 ℃ for 3 weeks for preparation of seed culture. One percent of seed culture was propagated in ISP-2 broth supplemented with 0.2% L-tryptophan and incubated in the dark at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in peptone water and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in modified Gaus No.1 broth and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. Catechol-type siderophores were determined by using Arnow's method (Arnow 1937) and hydroxamate siderophores were determined by using the Csaky test (Csaky 1984).
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Peptone water (30℃ + 21 days)
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Healthy leaves, shoots and root tissues | Nakhonnayok province and Phetchabun province, Thailand |
NP Content: 3 mg/ml
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| Species Name: Nonomuraea sp. S3310 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Peptone water | [1] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Healthy leaf, shoot and root tissues of Eaglewood (Aquilaria crassna Pierre ex Lec.) were collected from the plantations in Nakhonnayok province (14° 7′ 20.60″ N, 101° 4′ 16.23″ E) and Phetchabun province (16° 25′ 27.59″ N, 101° 9′ 42.25″ E) of Thailand, during the period of September-October 2008. The actinomycetes isolates were grown on HT agar at 30 ℃ for 3 weeks. Four-millimeter-diameter agar plugs were cut with a sterile cork borer from the leading edges of colonies, and one such plug was transferred into a 250 ml Erlenmeyer flask containing 100 ml of ISP-2 broth and incubated at 30 ℃ for 3 weeks for preparation of seed culture. One percent of seed culture was propagated in ISP-2 broth supplemented with 0.2% L-tryptophan and incubated in the dark at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in peptone water and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in modified Gaus No.1 broth and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. Catechol-type siderophores were determined by using Arnow's method (Arnow 1937) and hydroxamate siderophores were determined by using the Csaky test (Csaky 1984).
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Peptone water (30℃ + 21 days)
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Healthy leaves, shoots and root tissues | Nakhonnayok province and Phetchabun province, Thailand |
NP Content: 9 mg/ml
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| Species Name: Pseudonocardia halophobica strain S4201 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Peptone water | [1] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Healthy leaf, shoot and root tissues of Eaglewood (Aquilaria crassna Pierre ex Lec.) were collected from the plantations in Nakhonnayok province (14° 7′ 20.60″ N, 101° 4′ 16.23″ E) and Phetchabun province (16° 25′ 27.59″ N, 101° 9′ 42.25″ E) of Thailand, during the period of September-October 2008. The actinomycetes isolates were grown on HT agar at 30 ℃ for 3 weeks. Four-millimeter-diameter agar plugs were cut with a sterile cork borer from the leading edges of colonies, and one such plug was transferred into a 250 ml Erlenmeyer flask containing 100 ml of ISP-2 broth and incubated at 30 ℃ for 3 weeks for preparation of seed culture. One percent of seed culture was propagated in ISP-2 broth supplemented with 0.2% L-tryptophan and incubated in the dark at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in peptone water and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in modified Gaus No.1 broth and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. Catechol-type siderophores were determined by using Arnow's method (Arnow 1937) and hydroxamate siderophores were determined by using the Csaky test (Csaky 1984).
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Peptone water (30℃ + 21 days)
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Healthy leaves, shoots and root tissues | Nakhonnayok province and Phetchabun province, Thailand |
NP Content: 30 mg/ml
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| Species Name: Streptomyces hainanensis strain S4303 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Peptone water | [1] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Healthy leaf, shoot and root tissues of Eaglewood (Aquilaria crassna Pierre ex Lec.) were collected from the plantations in Nakhonnayok province (14° 7′ 20.60″ N, 101° 4′ 16.23″ E) and Phetchabun province (16° 25′ 27.59″ N, 101° 9′ 42.25″ E) of Thailand, during the period of September-October 2008. The actinomycetes isolates were grown on HT agar at 30 ℃ for 3 weeks. Four-millimeter-diameter agar plugs were cut with a sterile cork borer from the leading edges of colonies, and one such plug was transferred into a 250 ml Erlenmeyer flask containing 100 ml of ISP-2 broth and incubated at 30 ℃ for 3 weeks for preparation of seed culture. One percent of seed culture was propagated in ISP-2 broth supplemented with 0.2% L-tryptophan and incubated in the dark at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in peptone water and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in modified Gaus No.1 broth and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. Catechol-type siderophores were determined by using Arnow's method (Arnow 1937) and hydroxamate siderophores were determined by using the Csaky test (Csaky 1984).
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Peptone water (30℃ + 21 days)
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Healthy leaves, shoots and root tissues | Nakhonnayok province and Phetchabun province, Thailand |
NP Content: 60 mg/ml
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| Species Name: Streptomyces javensis strain S4202 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Peptone water | [1] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Healthy leaf, shoot and root tissues of Eaglewood (Aquilaria crassna Pierre ex Lec.) were collected from the plantations in Nakhonnayok province (14° 7′ 20.60″ N, 101° 4′ 16.23″ E) and Phetchabun province (16° 25′ 27.59″ N, 101° 9′ 42.25″ E) of Thailand, during the period of September-October 2008. The actinomycetes isolates were grown on HT agar at 30 ℃ for 3 weeks. Four-millimeter-diameter agar plugs were cut with a sterile cork borer from the leading edges of colonies, and one such plug was transferred into a 250 ml Erlenmeyer flask containing 100 ml of ISP-2 broth and incubated at 30 ℃ for 3 weeks for preparation of seed culture. One percent of seed culture was propagated in ISP-2 broth supplemented with 0.2% L-tryptophan and incubated in the dark at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in peptone water and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in modified Gaus No.1 broth and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. Catechol-type siderophores were determined by using Arnow's method (Arnow 1937) and hydroxamate siderophores were determined by using the Csaky test (Csaky 1984).
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Peptone water (30℃ + 21 days)
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Healthy leaves, shoots and root tissues | Nakhonnayok province and Phetchabun province, Thailand |
NP Content: 30 mg/ml
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| References | ||||||||||||||||||||||||
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| 1 | Endophytic actinomycetes isolated from Aquilaria crassna Pierre ex Lec and screening of plant growth promoters production | |||||||||||||||||||||||