General Information of Species (ID: SP0404)
  Species Name
Streptomyces javensis
  Species Genus Streptomyces
  Species Family Streptomycetaceae
  Microbe Type Endophytic Actinomycetes
  Studied Organism Streptomyces javensis strain S4202
  External Links
Taxonomy ID 114698
Genbank ID GQ179657
  Host Plant (s)
Aquilaria crassna Species Info

 The Content Variation of Natural Product Induced by Different Factor(s)
      ISP-2 medium [1]
              Factor Info Click to show the detail information of this Factor
               Experiment Detail
Healthy leaf, shoot and root tissues of Eaglewood (Aquilaria crassna Pierre ex Lec.) were collected from the plantations in Nakhonnayok province (14° 7′ 20.60″ N, 101° 4′ 16.23″ E) and Phetchabun province (16° 25′ 27.59″ N, 101° 9′ 42.25″ E) of Thailand, during the period of September-October 2008. The actinomycetes isolates were grown on HT agar at 30 ℃ for 3 weeks. Four-millimeter-diameter agar plugs were cut with a sterile cork borer from the leading edges of colonies, and one such plug was transferred into a 250 ml Erlenmeyer flask containing 100 ml of ISP-2 broth and incubated at 30 ℃ for 3 weeks for preparation of seed culture. One percent of seed culture was propagated in ISP-2 broth supplemented with 0.2% L-tryptophan and incubated in the dark at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in peptone water and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in modified Gaus No.1 broth and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. Catechol-type siderophores were determined by using Arnow's method (Arnow 1937) and hydroxamate siderophores were determined by using the Csaky test (Csaky 1984).
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  ISP-2 medium with 0.2% L-tryptophan (30℃ + 21 days)   (Part: Healthy leaves, shoots and root tissues; Location: Nakhonnayok province and Phetchabun province, Thailand)
               NP Name Link Host Plant Part Location NP Content
 
Indole-3-Acetic Acid
NP Info Aquilaria crassna Healthy leaves, shoots and root tissues Nakhonnayok province and Phetchabun province, Thailand
NP Content: 0.01055 ± 0.00023 mg/ml
      Peptone water [1]
              Factor Info Click to show the detail information of this Factor
               Experiment Detail
Healthy leaf, shoot and root tissues of Eaglewood (Aquilaria crassna Pierre ex Lec.) were collected from the plantations in Nakhonnayok province (14° 7′ 20.60″ N, 101° 4′ 16.23″ E) and Phetchabun province (16° 25′ 27.59″ N, 101° 9′ 42.25″ E) of Thailand, during the period of September-October 2008. The actinomycetes isolates were grown on HT agar at 30 ℃ for 3 weeks. Four-millimeter-diameter agar plugs were cut with a sterile cork borer from the leading edges of colonies, and one such plug was transferred into a 250 ml Erlenmeyer flask containing 100 ml of ISP-2 broth and incubated at 30 ℃ for 3 weeks for preparation of seed culture. One percent of seed culture was propagated in ISP-2 broth supplemented with 0.2% L-tryptophan and incubated in the dark at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in peptone water and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in modified Gaus No.1 broth and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. Catechol-type siderophores were determined by using Arnow's method (Arnow 1937) and hydroxamate siderophores were determined by using the Csaky test (Csaky 1984).
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  Peptone water (30℃ + 21 days)   (Part: Healthy leaves, shoots and root tissues; Location: Nakhonnayok province and Phetchabun province, Thailand)
               NP Name Link Host Plant Part Location NP Content
 
Ammonia
NP Info Aquilaria crassna Healthy leaves, shoots and root tissues Nakhonnayok province and Phetchabun province, Thailand
NP Content: 30 mg/ml
      MGs medium [1]
              Factor Info Click to show the detail information of this Factor
               Experiment Detail
Healthy leaf, shoot and root tissues of Eaglewood (Aquilaria crassna Pierre ex Lec.) were collected from the plantations in Nakhonnayok province (14° 7′ 20.60″ N, 101° 4′ 16.23″ E) and Phetchabun province (16° 25′ 27.59″ N, 101° 9′ 42.25″ E) of Thailand, during the period of September-October 2008. The actinomycetes isolates were grown on HT agar at 30 ℃ for 3 weeks. Four-millimeter-diameter agar plugs were cut with a sterile cork borer from the leading edges of colonies, and one such plug was transferred into a 250 ml Erlenmeyer flask containing 100 ml of ISP-2 broth and incubated at 30 ℃ for 3 weeks for preparation of seed culture. One percent of seed culture was propagated in ISP-2 broth supplemented with 0.2% L-tryptophan and incubated in the dark at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in peptone water and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in modified Gaus No.1 broth and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. Catechol-type siderophores were determined by using Arnow's method (Arnow 1937) and hydroxamate siderophores were determined by using the Csaky test (Csaky 1984).
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  MGs medium (Csaky assay) (30℃ + 21 days)   (Part: Healthy leaves, shoots and root tissues; Location: Nakhonnayok province and Phetchabun province, Thailand)
               NP Name Link Host Plant Part Location NP Content
 
Hydroxamate Siderophores
NP Info Aquilaria crassna Healthy leaves, shoots and root tissues Nakhonnayok province and Phetchabun province, Thailand
NP Content: 0.01534 ± 0.00013 mg/ml
References
1 Endophytic actinomycetes isolated from Aquilaria crassna Pierre ex Lec and screening of plant growth promoters production