| General Information of Species (ID: SP0402) | |||||||
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| Species Name |
Nocardia jiangxiensis
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| Species Genus | Nocardia | ||||||
| Species Family | Nocardiaceae | ||||||
| Microbe Type | Endophytic Actinomycetes | ||||||
| Studied Organism | Nocardia jiangxiensis strain S3311 | ||||||
| External Links | |||||||
| Taxonomy ID | 282685 | ||||||
| Genbank ID | GQ179659 | ||||||
| Host Plant (s) | |||||||
| Aquilaria crassna | Species Info | ||||||
| The Content Variation of Natural Product Induced by Different Factor(s) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| ISP-2 medium | [1] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Factor Info
Click to show the detail information of this Factor
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| Experiment Detail |
Healthy leaf, shoot and root tissues of Eaglewood (Aquilaria crassna Pierre ex Lec.) were collected from the plantations in Nakhonnayok province (14° 7′ 20.60″ N, 101° 4′ 16.23″ E) and Phetchabun province (16° 25′ 27.59″ N, 101° 9′ 42.25″ E) of Thailand, during the period of September-October 2008. The actinomycetes isolates were grown on HT agar at 30 ℃ for 3 weeks. Four-millimeter-diameter agar plugs were cut with a sterile cork borer from the leading edges of colonies, and one such plug was transferred into a 250 ml Erlenmeyer flask containing 100 ml of ISP-2 broth and incubated at 30 ℃ for 3 weeks for preparation of seed culture. One percent of seed culture was propagated in ISP-2 broth supplemented with 0.2% L-tryptophan and incubated in the dark at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in peptone water and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in modified Gaus No.1 broth and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. Catechol-type siderophores were determined by using Arnow's method (Arnow 1937) and hydroxamate siderophores were determined by using the Csaky test (Csaky 1984).
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| ISP-2 medium with 0.2% L-tryptophan (30℃ + 21 days) (Part: Healthy leaves, shoots and root tissues; Location: Nakhonnayok province and Phetchabun province, Thailand) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| NP Name | Link | Host | Plant Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Indole-3-Acetic Acid
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NP Info | Aquilaria crassna | Healthy leaves, shoots and root tissues | Nakhonnayok province and Phetchabun province, Thailand |
NP Content: 0.01035 ± 0.00009 mg/ml
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| Peptone water | [1] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Factor Info
Click to show the detail information of this Factor
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| Experiment Detail |
Healthy leaf, shoot and root tissues of Eaglewood (Aquilaria crassna Pierre ex Lec.) were collected from the plantations in Nakhonnayok province (14° 7′ 20.60″ N, 101° 4′ 16.23″ E) and Phetchabun province (16° 25′ 27.59″ N, 101° 9′ 42.25″ E) of Thailand, during the period of September-October 2008. The actinomycetes isolates were grown on HT agar at 30 ℃ for 3 weeks. Four-millimeter-diameter agar plugs were cut with a sterile cork borer from the leading edges of colonies, and one such plug was transferred into a 250 ml Erlenmeyer flask containing 100 ml of ISP-2 broth and incubated at 30 ℃ for 3 weeks for preparation of seed culture. One percent of seed culture was propagated in ISP-2 broth supplemented with 0.2% L-tryptophan and incubated in the dark at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in peptone water and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in modified Gaus No.1 broth and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. Catechol-type siderophores were determined by using Arnow's method (Arnow 1937) and hydroxamate siderophores were determined by using the Csaky test (Csaky 1984).
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| Peptone water (30℃ + 21 days) (Part: Healthy leaves, shoots and root tissues; Location: Nakhonnayok province and Phetchabun province, Thailand) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| NP Name | Link | Host | Plant Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Ammonia
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NP Info | Aquilaria crassna | Healthy leaves, shoots and root tissues | Nakhonnayok province and Phetchabun province, Thailand |
NP Content: 25 mg/ml
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| MGs medium | [1] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Factor Info
Click to show the detail information of this Factor
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| Experiment Detail |
Healthy leaf, shoot and root tissues of Eaglewood (Aquilaria crassna Pierre ex Lec.) were collected from the plantations in Nakhonnayok province (14° 7′ 20.60″ N, 101° 4′ 16.23″ E) and Phetchabun province (16° 25′ 27.59″ N, 101° 9′ 42.25″ E) of Thailand, during the period of September-October 2008. The actinomycetes isolates were grown on HT agar at 30 ℃ for 3 weeks. Four-millimeter-diameter agar plugs were cut with a sterile cork borer from the leading edges of colonies, and one such plug was transferred into a 250 ml Erlenmeyer flask containing 100 ml of ISP-2 broth and incubated at 30 ℃ for 3 weeks for preparation of seed culture. One percent of seed culture was propagated in ISP-2 broth supplemented with 0.2% L-tryptophan and incubated in the dark at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in peptone water and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. One percent of seed culture was propagated in modified Gaus No.1 broth and incubated at 30 ℃ with shaking at 120 rev/min for 3 weeks. Catechol-type siderophores were determined by using Arnow's method (Arnow 1937) and hydroxamate siderophores were determined by using the Csaky test (Csaky 1984).
Click to Show/Hide
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| MGs medium (Csaky assay) (30℃ + 21 days) (Part: Healthy leaves, shoots and root tissues; Location: Nakhonnayok province and Phetchabun province, Thailand) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| NP Name | Link | Host | Plant Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Hydroxamate Siderophores
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NP Info | Aquilaria crassna | Healthy leaves, shoots and root tissues | Nakhonnayok province and Phetchabun province, Thailand |
NP Content: 0.00503 ± 0.00033 mg/ml
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| References | ||||||||||||||||||||||||
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| 1 | Endophytic actinomycetes isolated from Aquilaria crassna Pierre ex Lec and screening of plant growth promoters production | |||||||||||||||||||||||
