| The Content Variation of Natural Product Induced by Different Factor(s) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Species Name: Artemisia absinthium | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Chemotype Comparison | [1] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Ten different plants of wormwood were collected in March 1997 from each one of the following four wild populations in the Spanish Pyrenees: Tallo de Aulet (prov. Huesca) and Pont de Suert, Sort and Farga de Moles (prov. Lleida). In three of the four populations studied, there was another chemotype, with 25-65% of cis-epoxyocimene and 15-50% of chrysanthenyl acetate. This chemotype, called chemotype B, was less frequent in the Pyrenees than the chemotype A, appearing only in 17% of the samples (two samples in TallO de Aulet and in Pont de Suert and three samples in Farga de Moles).
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| Factor Function |
Two chemotypes were detected; a cis-epoxyocimene type (with more than 50% of this compound) which was predominant in all the populations, and a cis-epoxyocimene + chrysanthenyl acetate type (with 25-65% of cis-epoxyocimene and 15-50% of chrysanthenyl acetate). The distribution of these chemotypes had no relation with the altitude of the samples.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Chemotype (cis-epoxyocimene type)
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Leaves | Spain |
NP Content: <0.03 %
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Chemotype (cis-epoxyocimene + chrysanthenyl acetate type)
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Leaves | Spain |
NP Content: 0.05 %
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| Species Name: Artemisia campestris | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Locality Variation | [2] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
The aerial parts (~20 cm, 15-100 g) of A. campestris L. from ten different wild populations of Lithuania were gathered at the full flowering stage. Plant material was dried at room temperature (20-25 ℃). Oils (samples 1-10) obtained from Artemisia campestris plants collected at sampling sites (A-I,Y) characterized by locality, city (c.) or district (d.), soil type (Or, ordo; Sn, sand; Sl, sandy loam; Gr, gravel; Lm, loam) and description of natural habitat (Af, abandoned field; Fe, forest edge; Ct, cutting area; Mw, meadow; Rs, roadside; Rv, river valley): A (1) Birstonas c. (Or, Ct); B (2) Palanga c. (Sn, Fe); C (3) Nociunai, Kedainai d. (Or, Mw); D (4) Alytus c. (Sl, Rs); E (5) Moletai c. (Lm, Af); F (6) Kaltanenai, Sencionys d. (Gr, Fe); G (7) Merkine, Alytus d. (Sl, Ct); H (8) Trakai c. (Gr, Af); I (9) Druskininkai c. (Or, Rv); Y (10) Vilnius c. (Gr, Af).
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| Factor Function |
The main chemical profile (ten samples) was characterized by the predominance of germacrene D (9.8-31.2%), while spathulenol, humulene epoxide II and caryophyllene oxide were found as the first major compounds in another three oils. One oil was determined as a mixed chemotype. Some compounds such as gamma-curcumene, alpha-cadinol, (E,E)-alpha-farnesene, beta-ylangene, beta-selinene and humulene epoxide II have been mentioned for the first time among three principal constituents in A. campestris oils. The fifty-six components made up 73.6.1-98.5% of the total content, while the remaining twenty-six volatile compounds were identified in insignificant amounts in the A. campestris essential oils.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Locality: Birstonas city, Lithuania (soil type: ordo; natural habitat: cutting area)
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Aerial parts | Lithuania |
NP Content: 0.2 %
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Locality: Moletai city, Lithuania (soil type: loam; natural habitat: abandoned field)
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Aerial parts | Lithuania |
NP Content: 0.3 %
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Locality: Kaltanenai, Sencionys district, Lithuania (soil type: gravel; natural habitat: forest edge)
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Aerial parts | Lithuania |
NP Content: <0.05 %
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Locality: Vilnius city, Lithuania (soil type: gravel; natural habitat: abandoned field)
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Aerial parts | Lithuania |
NP Content: 0.2 %
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| Species Name: Artemisia ferganensis | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Developmental Stage Variation | [3] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
The leaves of aerial parts were collected in Heshuo county of Xinjiang province in China in July 2003 (a vegetative stage), June 2003 (a budding stage); and August 2003 (a flowering stage), respectively.
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| Factor Function |
Only 23 constituents were present at the budding stage, while 24 and 26 at the flowering and vegetative stages, respectively. p-Cymene and gamma-terpinene were not detected at the vegetative stage of the plant. During the budding stage, butyric, beta-caryophyllene, geranyl acetate and cis-jasmone could not be detected. Benzaldehyde was observed only at the vegetative stage. Variations were also observed in quantity. In all cases the analyzed oils were characterized by the high concentration of alpha-thujone, ranging in amount from 37.0% at the vegetative stage to 54.8% at the budding stage. The concentration of alpha-thujone at the flowering stage (49.0%) was lower than the budding stage, but higher than the vegetative stage. The concentration of cis-chrysanthenyl acetate varied between 23.5% and 7.2%, respectively, at the vegetative and budding stages. At the vegetative stage the concentration of 1,8-cineole was observed to be the lowest. It was highest at the budding stage, representing 10.4%, then decreased gradually to 8.8% at the flowering stage. The concentration of beta-thujone was relatively low at the vegetative stage, representing 8.6%, and then increased to 10.5% at the budding stage. When flowers appeared it was found to vary a little. Finally, the concentration of sabinyl acetate ranged from 10.2% (vegetative stage) to 6.5% (flowering stage).
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Vegetative stage
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Leaves | Heshuo, Xinjiang, China |
NP Content: <0.10 %
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Aerial part: Flowering stage
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Leaves | Heshuo, Xinjiang, China |
NP Content: 0.3 %
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| Species Name: Baccharis spartioides | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Altitude Variation | [4] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Aerial parts of endemic pichana were harvested in December 1996 at different localities of northern Patagonia. Origin: Planicie Banderita, Dept. Confluencia, Province of NeuquCn. Habitat: altitude, 327 m; average temperature in the station, 21.8 ℃; annual precipitation, 125 mm; sandy soils. Aerial parts (5 kg, 2 kg of dried material;humidity, 11%) from four well developed plants at the fullflowering stage (December, 1996). Sample 2 : Origin: RincBn de 10s; Sauces, Dept. of Pehuenclies, Province of Neuqukn. Habitat: altitude, 750 m; average temperature in the station, 20.9 ℃; annual precipitation, 147 mm; sandy and gritty salty soils. Aerial parts (5 kg, 1.85 kg of dried material, humidity, 10%), from two well developed plants at the full flowering stage, and after several days copious rains (December, 1996). Sample 3: Origin: Coronel GBmez, Dept. General Roca, Province of Rio Negro. Habitat: altitude, 242 m; average temperature in the station, 22.5 ℃; annual precipitation, 179 mm; sandy and stony soils. Aerial parts (4.5 kg, 1.3 kg of dried material, humidity, 9%), from 12 young plants at the beginning flowering stage (December, 1996).
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| Factor Function |
Fifty-four components, representing approximately 84.6-97.4% of the oil samples, were identified. The samples consisted mainly of hydrocarbons and oxygenated monoterpenes. The major constituents were limonene (28.7-56.7%), 6R-7R-bisabolone (3.2-9.1%), sabinene (0.1-11.0%) and citronellal (2.4-5.2%). Significant differences among the content of the three samples could be the result of changes in the climatic conditions (sample 2: Rincon de los Sauces, Province of Neuquen, after strong rains) or by translocations in different parts of the plant (sample 3: Coronel Gomez, Province of Rio Negro, more leaves and less stems).
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Locality: Planicie Banderita, Province of Neuquen; Altitude 327 m
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Aerial parts | Patagonia, Argentina |
NP Content: 1.3 %
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Locality: Rincon de los Sauces, Province of Neuquen; Altitude 750 m
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Aerial parts | Patagonia, Argentina |
NP Content: 0.1 %
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Locality: Coronel Gomez, Province of Rio Negro; Altitude 242 m
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Aerial parts | Patagonia, Argentina |
NP Content: 0.1 %
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| Species Name: Citrus aurantifolia | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Developmental Stage Variation | [5] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Fresh mature lime fruits were harvested from experimental orchards of I.I.H.R., Bangalore at six ripening stages: Peel color; Dark Green, Light Green, Color Turning, 1/2 Yellow, 3/4th Yellow and Full Yellow.
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| Factor Function |
The constituents of lime oil mainly belong to two categories: hydrocarbons and oxygenated compounds. The hydrocarbons were 85.4% of the peel oil isolated from full yellow fruits compared to 57.5% in green fruits. The most abundant monoterpene hydrocarbons, limonene and beta-pinene, showed gradual increase during ripening of lime fruit and they together accounted for 70.7% in full yellow fruits. Organoleptically important oxygenated compounds (neral, geranial, linalool and geraniol) were found to be rich in oil isolated from the peel of green fruits (29.7%); however, it decreased to 8.4% when color of the fruit turned to full yellow. Neral and geranial were found to be high in the peel oil of green fruits (7.8%) compared to full yellow fruits (2.5%).
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Fruit: Dark green stage
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Fruits (dark green) | Bangalore, India |
NP Content: 3 %
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Fruit: Light green stage
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Fruits (light green) | Bangalore, India |
NP Content: 2 %
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Fruit: Color turning stage
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Fruits (color turn) | Bangalore, India |
NP Content: 1.2 %
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Fruit: Half yellow stage
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Fruits (half yellow) | Bangalore, India |
NP Content: 0.7 %
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Fruit: 3/4th yellow stage
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Fruits (3/4th yellow) | Bangalore, India |
NP Content: 0.7 %
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Fruit: Full yellow stage
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Fruits (full yellow) | Bangalore, India |
NP Content: 0.6 %
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| Species Name: Citrus sinensis (Hongjiang) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Variety Comparison | [6] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Four kinds of fresh sweet oranges were obtained in the same season, November 2000, in Guangzhou. Citrus sinensis var. Hongjiang (called 'hong jiang chen' in Chinese) and C. sinensis Osbeck var. Anliu (called 'luo gang chen') were obtained at an orchard in Luo gang in Guangzhou (25 km from the center of Guangzhou). Citrus sinensis var. Sihui (called 'sihui ju') was harvested at the Shigou Experimental Farm in Sihui City in Guangdong Province (75 km far away from Guangzhou). Citrus sinensis var. Washington navel (called 'qi chen') which was produced in Jiangxi Province (200 km from Guangzhou; bordering Guangdong Province), was purchased at the wholesale market in Guangzhou. All oranges were kept in a cold room until prepared a few days later.
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| Factor Function |
The peel oil compositions of four kinds of sweet oranges in China, Citrus sinensis Osbeck var. Hongjian, C. sinensis Osbeck var. Anliu, C. sinensis Osbeck var. Sihui and C. sinensis Osbeck var. Washington navel, were investigated by GC and GC/MS. The essential oils were extracted by cold-pressing method. Forty-two to 53 compounds were quantitatively determined for each variety. Their percentages, respectively, were: > 97.3%, > 98.4%, > 97.5% and > 98.0% in hydrocarbons; > 1.5%, > 0.7%, > 0.8% and > 0.9% in total aldehydes; 0.8%, 0.5%, 0.5% and 0.5% in alcohols. Either cis-or trans-limonene oxide was detected in small amounts in each of the four samples, with Hongjiang containing both limonene oxides. delta-3-Carene was commonly quantified at a level of 0.1% in all the samples. The content of aliphatic aldehydes, including octanal, nonanal, decanal and dodecanal, exceeded that of terpene aldehydes, such as neral and geranial in Hongjiang (0.9%) and Washington navel (0.6%), whereas the aliphatic aldehydes in Anliu and Sihui were present to a lesser degree than the terpene aldehydes. Either alpha- or beta-sinensal was detected in trace amounts in each of the four samples. Linalool was the major alcohol in all the samples. Nootkatone was not detected.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Citrus sinensis var. Anliu
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Fruits | China |
NP Content: < 0.005 %
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Citrus sinensis var. Hongjiang
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Fruits | China |
NP Content: < 0.005 %
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Citrus sinensis var. Sihui
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Fruits | China |
NP Content: < 0.005 %
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Citrus sinensis var. Washington navel
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Fruits | China |
NP Content: < 0.005 %
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| Species Name: Coriandrum sativum | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Locality Variation | [7] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Two samples (20 kg each) of mature coriander (Coriandrum sativum L.) fruits were used for this study. The first was purchased from a spice market of Korba in Tunisia (Tn), the second, from Canada (Can), was supplied by General Herboristerie Laboratory (Marseille, France).
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| Factor Function |
The first from Tunisia (Tn) and the second from Canada (Can). The highest essential oil yield was observed for Can with 0.44% (w/w) and 0.37% (w/w) for Tn. Forty-five compounds were identified in the essential oils and the main compound of both samples was linalool. The total phenol contents varied between two coriander fruit samples; Can sample presented high polyphenol contents (15.16 mg GAE/g) compared with Tn one (12.10 mg GAE/g). Significant differences were also found in total tannin contents among representing 0.7 mg GAE/g in Can and 0.34 mg GAE/g in Tn. The highest contents of total flavonoids were observed in Can sample with 13.2 mg CE/g.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Locality: Canada
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Fruits | Canada |
NP Content: 2.9 %
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Locality: Korba, Tunisia
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Fruits | Tunisia |
NP Content: 1.8 %
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| Factor Name: Locality Variation | [8] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Fruits of coriander of commercial crops from Viamonte (Province of Cordoba), Argentina were compared with three Russian oils imported by the Argentinian fragrance and flavor industry.
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| Factor Function |
Twenty components were identified which accounted for 96.6-99-7% of the total oils composition. The main constituents were linalool (68.9-83-7%), gamma-terpinene (2,2-5.1%), camphor (3.2-4.8%), alpha-pinene (1.0-6.5%), geraniol (1.4-3.2%) and geranyl acetate (0.8-3.8%). The contents of cis- and trans-linalool oxide (0.1-0.4%) were low.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Locality: Russia
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Fruits | Russia |
NP Content: 2.4 %
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Locality: Viamonte, Province of Cordoba, Argentina
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Fruits | Argentina |
NP Content: 1.3 %
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| Species Name: Cymbopogon martinii | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Ethyl methane sulfonate Treatment; Ethyleneimine Treatment; Gamma ray Treatment; Cultivar Comparison | [9] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
The materials (dry palmarosa seeds with 12% moisture content of four cultivars 'Tripta', 'Trishna', 'PRC' and 'MP') and methods of gamma rays (15 Kr, 60CO source with dose rate 0.38 Mrads\h) and chemicals ethyl methane sulfonate (EMS - 0.4%) and ethyleneimine (EI-0.04%) induced mutagenesis. The seed of the four varieties had already been selfed for 4-5 flowering seasons before mutagenesis to maintain genetic homogeneity (or purity) and thereafter all the selected M1 generation suspected mutant plants were individually selfed by bagging to give rise to controlled M2 plant to progeny segregants for further selection. Oil content was estimated on freshly harvested herbage (stems, leaves and inflorescence) using a Clevenger-type apparatus.
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| Factor Function |
The oil content was increased in all the 20 mutants as compared to their respective contols. Most M2 generation mutants were found to exhibit a straight relationship between high herbage (stem, leaves and inflorescence) yield, oil content (%) and oil quality in terms of major and trace constituents of the oil. Six mutants specifically were endowed with the desirable rosy note which remained predominant in the samples of Trishna-gamma-5, MP-gamma-13, Tripta-gamma-8, Tripta-Ethyl methane sulfonate (EMS)-19, Tripta-EMS-21 and PRC-Ethyleneimine (EI)-44. The fresh herbage and oil yield and odor criteria, i.e., rosy note were satisified by the three best mutants, viz., Trishna-gamma-5, MP-gamma-13 and Tripta-gamma-19. The results have been interpreted in the sense that induction of mutations brings about gene level changes from dominance to recessive and vice versa in morpho-economic traits having quantitative trait loci (QTL) under polygenic genetic control.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Cymbopogon martinii cv. MP (Control)
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Herbages | India |
NP Content: 2.55 %
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Mutant 31: C. martinii cv. MP induced by ethyl methane sulfonate
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Herbages | India |
NP Content: 20.31 %
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Mutant 31: C. martinii cv. MP induced by ethyl methane sulfonate
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Herbages | India |
NP Content: 38.04 %
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Mutant 13: C. martinii cv. MP induced by gamma rays
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Herbages | India |
NP Content: 8.93 %
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Cymbopogon martinii cv. PRC (Control)
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Herbages | India |
NP Content: 2.31 %
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Mutant 43: C. martinii cv. PRC induced by ethyleneimine
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Herbages | India |
NP Content: 10.82 %
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Mutant 44: C. martinii cv. PRC induced by ethyleneimine
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Herbages | India |
NP Content: 10.19 %
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Mutant 45: C. martinii cv. PRC induced by ethyleneimine
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Herbages | India |
NP Content: 14.18 %
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Mutant 48: C. martinii cv. PRC induced by ethyleneimine
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Herbages | India |
NP Content: 15.6 %
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Mutant 9: C. martinii cv. PRC induced by gamma rays
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Herbages | India |
NP Content: 6.36 %
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Mutant 10: C. martinii cv. PRC induced by gamma rays
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Herbages | India |
NP Content: 17.57 %
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Mutant 12: C. martinii cv. PRC induced by gamma rays
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Herbages | India |
NP Content: 18.93 %
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Cymbopogon martinii cv. Tripta (Control)
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Herbages | India |
NP Content: 1.89 %
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Mutant 18: C. martinii cv. Tripta induced by ethyl methane sulfonate
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Herbages | India |
NP Content: 9.34 %
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Mutant 19: C. martinii cv. Tripta induced by ethyl methane sulfonate
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Herbages | India |
NP Content: 9.65 %
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Mutant 20: C. martinii cv. Tripta induced by ethyl methane sulfonate
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Herbages | India |
NP Content: 25.16 %
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Mutant 21: C. martinii cv. Tripta induced by ethyl methane sulfonate
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Herbages | India |
NP Content: 13.29 %
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Mutant 1: C. martinii cv. Trishna induced by gamma rays
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Herbages | India |
NP Content: 9.99 %
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Mutant 2: C. martinii cv. Trishna induced by gamma rays
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Herbages | India |
NP Content: 22.02 %
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Cymbopogon martinii cv. Trishna (Control)
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Herbages | India |
NP Content: 3.55 %
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Mutant 41: C. martinii cv. Trishna induced by ethyleneimine
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Herbages | India |
NP Content: 16.29 %
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Mutant 5: C. martinii cv. Trishna induced by gamma rays
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Herbages | India |
NP Content: 12.71 %
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Mutant 6: C. martinii cv. Trishna induced by gamma rays
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Herbages | India |
NP Content: 5.5 %
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Mutant 8: C. martinii cv. Trishna induced by gamma rays
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Herbages | India |
NP Content: 20.85 %
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| Species Name: Cymbopogon nardus | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Gamma ray Treatment | [10] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Dormant vegetative slips of a single clone of Jamrosa of same age and size were subjected to various doses of gamma-ray treatment, doses ranging from 3-10 kR in three replications. Radiation source was gamma cell operating at dose rate of 2.0 R/minute. The irradiated clones along with the parental clones were planted in the experimental fields. Plants raised from irradiated plants were very carefully screened through visual and chemical analysis and were individually harvested.
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| Factor Function |
Examination of the data reveals that highest oil yield per hectare was obtained from variant RL-931 giving a 33% increase in the oil yield over the parent close. This variant clone (RL-931) also had the desired reduction of the neral/geranial content in its oil coupled with increase in total alcohol content calculated as geraniol and geranyl acetate in the oil. Mutant RL-921 had a significantly higher percentage of geranyl acetate content in its oil compared to its control parental clone. Among the five mutant clones, the lowest neral/geranial content was found in clone RI-924, however, it also had reduced level of geraniol and geranyl acetate.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Mutant clone RL-82 (Parental): subjected to gamma-ray treatment
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Whole plant | India |
NP Content: 43.3 %
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Mutant clone RL-921: subjected to gamma-ray treatment
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Whole plant | India |
NP Content: 56.2 %
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Mutant clone RL-922: subjected to gamma-ray treatment
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Whole plant | India |
NP Content: 29.4 %
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Mutant clone RL-923: subjected to gamma-ray treatment
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Whole plant | India |
NP Content: 46.1 %
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Mutant clone RL-924: subjected to gamma-ray treatment
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Whole plant | India |
NP Content: 30.3 %
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Mutant clone RL-931: subjected to gamma-ray treatment
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Whole plant | India |
NP Content: 41.8 %
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| Species Name: Cymbopogon winterianus | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Pest Infestation | [11] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
General plantation of citronella cv. Java 2 was maintained following recommended agricultural practices at the Experimental Farm of Central Institute of Medicinal and Aromatic Plants, Field Station, Hyderabad, India. The experimental station has a semi-arid tropical climate. The experiment was conducted in the same plantation for 2 consecutive years during the summer month of June 1996 and 1997, when the incidence of the disease was higher. In each year, 12 each of healthy and diseased plants were selected at random and harvested. The occurrence of the disease is generally observed during the hot summer season months, when the temperatures are in the range 36-43 ℃. Initial symptoms of the pest attack appear as yellow specks or blotches, mostly along leaf margins, that in later stages develop into yellow streaks running along the length of the affected leaves. Emerging young leaves are pale green to yellow coloured, twisted, crinkled, developed into whip-like structures and in severe cases of infection fail to open. Even if they do open, these leaves fail to exhibit a smooth leaf surface. Severely affected older leaves turn brown, dry and die. The overall growth and development of the infected plant is severely affected, giving it a dwarfed and unhealthy appearance.
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| Factor Function |
The essential oil examined by GC and GC-MS from cultivated healthy plants contained citronellal (28.4%), geraniol (24.8%), citronellol (11.8%) and elemol (10.2%). The major components from diseased plants were geraniol (19.0-25.5%), elemol (15.3-20.4%), citronellal (13.4-19.1%) and citronellol (12.9-15.1%). Caryophyllene oxide (3.5-6.0%) was an important minor component.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Healthy leaves of healthy plant
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Leaves | Hyderabad, India |
NP Content: 4.7 %
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Healthy leaves of diseased plant (Yellowing and crinkling disease)
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Leaves | Hyderabad, India |
NP Content: 2.5 %
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Crinkled, whip like leaves of diseased plant (Yellowing and crinkling disease)
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Leaves | Hyderabad, India |
NP Content: 3.2 %
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Dead leaves of diseased plant (Yellowing and crinkling disease)
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Leaves | Hyderabad, India |
NP Content: 1.4 %
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Semi-diseased leaves of diseased plant (Yellowing and crinkling disease)
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Leaves | Hyderabad, India |
NP Content: 2.4 %
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| Species Name: Ducrosia anethifolia | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Locality Variation | [12] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
The aerial parts of Ducrosia anethifolia (DC.) Boiss. were collected in the wild from Mehdi Abad (Kerman province, in southern Iran) at the flowering stage in June 2006. The material was dried at room temperature.
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| Factor Function |
The 63 components of this interesting plant were identified in the oil of D. anethifolia, representing 94.0% of the oil. alpha-Pinene (11.6%), terpinolene(3.2%) and (z)-beta-ocimene (2.8%) were the main hydrocarbon components present in the oil, while decanal (54.0%), cis-chrysanthenyl acetate(3.2%) and decanoic acid (1.3%) were the major oxygen-containing constituents.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Locality: Karaj, Iran
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Aerial parts | Iran |
NP Content: <0.05 %
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Locality: Kerman, Iran
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Aerial parts | Iran |
NP Content: 0.3 %
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| Species Name: Ducrosia assadii | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Locality Variation | [13] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
The aerial parts of D. assadii Alava. were collected in the wild from Lalehzar (Kerman Province, in southern Iran) at the flowering stage, in July 2007. The material was dried at room temperature and used for distillation. Distillation: A direct-fired field distillation unit containing a distillation tank (capacity: 1,000 L), a condensation column and receiver, all made of stainless steel, and which can process 30-50 kg of dried aerial parts from the plants/batch, was installed at an altitude of 2600 m (boiling point: 87 ℃). Dried aerial parts from the plants (40 kg) were charged into the distillation unit along with 500 L fresh water and the unit was heated by steam. The system was kept open to atmospheric pressure until the temperature reached to 70 ℃, when the air present in the unit was replaced by the vapor. After complete removal of air from the unit, the air vent was closed and the whole unit was operated as a closed system under pressure to distill the oil. The pressure, temperature and rate of distillation were controlled manually. The process was completed after the collection of 500 L of water distillate. The oil collected in the receiver and dried over anhydrous Na2SO4. Extraction of Ducrosia Second Oil From Ducrosia Water by Redistillation: The seprated distillate water collected in the receiver was redistilled in a 1,000 L still to yield more Doucrosia oil (this oil is known as secondary essential oil, second oil, cooked oil or indirect oil).
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| Factor Function |
Fifty components were identified in a second oil of D. assadii from Lalehzar with decanal (35.2%), nonadecane (12%) and citronellyl acetate (11.6%) as the main constituents. The oil from Dehbakrii also contained decanal (36.4%) as the main component of an oil recovered from the distillate water. The results showed that the amount of decanal is remarkably high in the oils of D. assadii.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Locality: Lalehzar, Kerman Province, southern Iran
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Aerial parts | Iran |
NP Content: 0.4 %
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| Species Name: Eucalyptus urophylla | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Seasonal Variation | [14] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Eucalyptus urophylla and E. grandis were collected in January (summer) and August (winter) 2006 at the mature vegetative state from Goiania city Brazil, and identified by one of the authors (E.P.F.). Leaves from 5-11 randomized individual plants of the same age representing the local population were collected as homogenous samples in each season, dried at room temperature.
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| Factor Function |
The results were submitted to Principal Components and Clusters Analysis which enabled four groups of oils to be distinguished with regard to specimens and harvest seasons: clusters I and II with only E. grandis samples collected in the cold and dry winter and the hot and humid summer, which were characterized by a high percentage of isoleptospermone (9.6% and 13.2%), alpha-pinene (12.2% and 24.7%), p-cymene (20.5% and 14.5%), and alpha-terpineol (14.3% and 4.9%), respectively; clusters III and IV only associated with E. urophylla samples collected in summer and winter with 1,8-cineole (36.6% and 44.7%) and alpha-terpinyl acetate (7.0% and 11.7%) rich oils.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Harvesting time: Summer
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Whole plant | Brazil |
NP Content: 0.3 %
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Harvesting time: Winter
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Whole plant | Brazil |
NP Content: 0.3 %
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| Species Name: Melampodium camphoratum | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Locality Variation | [15] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
The aerial parts of M. camphoratum were collected at Manaus, Amazonas (type A) and Vigia, Para, (type B).
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| Factor Function |
The plants were collected from two different localities in the Amazon Region and their oils were found to be remarkably different. One oil obtained from the sample collected at Manaus was characterized by a high content of terpinolene (30.3%), limonene (13.8%) and delta-3-carene (13.2%). The main constituents found in the other oil distilled from a sample collected at Vigia were camphor (15.0%), alpha-phellandrene (20.5%) and beta-caryophyllene (8.9%)
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Locality: Manaus, Amazonas, Brazil
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Aerial parts | Brazil |
NP Content: 3.7 %
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| Species Name: Mentha spicata | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Month Variation | [16] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Plant material: Leaves of M. spicata plants were collected from a wild population of Mt. Pangeon (alt. 600 m, 40° 55′ N/ 24° 12′ E). Collections were conducted every month during the growing period (April to October).
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| Factor Function |
The oil content ranged from 0.1-1.8%, with the maximum values in late summer/early autumn. The essential oil obtained from the leaves was characterized by a very high content in linalool, i.e. 85.0-93.9% of the total oil (highest percentage in mid-autumn). Other oil constituents occurring in much lower amounts were germacrene D (up to 4.2%), beta-caryophyllene (up to 2.6%) and 1,8-cineole (up to 2.1%).
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Harvesting time: May
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Leaves | Greece |
NP Content: <0.05 %
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Harvesting time: June
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Leaves | Greece |
NP Content: <0.05 %
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Harvesting time: August
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Leaves | Greece |
NP Content: <0.05 %
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Harvesting time: September
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Leaves | Greece |
NP Content: <0.05 %
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| Species Name: Micromeria biflora | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Seasonal Variation | [17] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
The aerial parts of M. biflora collected during November 1993 and June 1994 were used for the investigation.
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| Factor Function |
The major constituents of the oil were neral (25.3-32.2%) and geranial (26.7-41.3%). The oil produced in the winter was found to contain higher amounts of oxygenated monoterpenes than the oil produced in the summer.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Harvesting time: Summer
|
Aerial parts | South India |
NP Content: 1.56 %
|
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Harvesting time: Winter
|
Aerial parts | South India |
NP Content: 0.85 %
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Species Name: Myrtus communis var. italica | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Month Variation | [18] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Myrtle (M. communis var. italica) aerial parts were collected monthly during 2006-2007 from Jbal Stara of Haouaria region in North Tunisia, belonging to a subhumid bioclimate.
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| Factor Function |
In conclusion, high fluctuations were observed in the oil yields and composition of different parts of Myrtus communis var. italica during all the collecting periods. They could be explained by genetic and environmental factors. Moreover, significant differences were revealed in the main oil compounds. alpha-Pinene percentages showed the most remarkable changes among the different part oils. So, leaf oils contained more alpha-pinene than those of the fruits and stems during the myrtle vegetative cycle.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Leaf: (Harvesting time: January)
|
Leaves | Tunisia |
NP Content: 0.5 %
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Leaf: (Harvesting time: February)
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Leaves | Tunisia |
NP Content: 0.4 %
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Leaf: (Harvesting time: March)
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Leaves | Tunisia |
NP Content: 0.8 %
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||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Leaf: (Harvesting time: April)
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Leaves | Tunisia |
NP Content: 1.6 %
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Leaf: (Harvesting time: May)
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Leaves | Tunisia |
NP Content: 1.8 %
|
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Leaf: (Harvesting time: June)
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Leaves | Tunisia |
NP Content: 1.8 %
|
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Leaf: (Harvesting time: July)
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Leaves | Tunisia |
NP Content: 1.3 %
|
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Leaf: (Harvesting time: August)
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Leaves | Tunisia |
NP Content: 1 %
|
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Leaf: (Harvesting time: September)
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Leaves | Tunisia |
NP Content: 1.1 %
|
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Leaf: (Harvesting time: October)
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Leaves | Tunisia |
NP Content: 2.2 %
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Leaf: (Harvesting time: November)
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Leaves | Tunisia |
NP Content: 2.8 %
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Leaf: (Harvesting time: December)
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Leaves | Tunisia |
NP Content: 1.6 %
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Fruit: (Harvesting time: January)
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Fruits | Tunisia |
NP Content: 2.1 %
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Fruit: (Harvesting time: August)
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Fruits | Tunisia |
NP Content: 20.5 %
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Fruit: (Harvesting time: September)
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Fruits | Tunisia |
NP Content: 1.8 %
|
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Fruit: (Harvesting time: October)
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Fruits | Tunisia |
NP Content: 7 %
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Fruit: (Harvesting time: November)
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Fruits | Tunisia |
NP Content: 10.9 %
|
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Fruit: (Harvesting time: December)
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Fruits | Tunisia |
NP Content: 6.5 %
|
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Stem: (Harvesting time: January)
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Stems | Tunisia |
NP Content: 0.9 %
|
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Stem: (Harvesting time: February)
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Stems | Tunisia |
NP Content: 0.2 %
|
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Stem: (Harvesting time: March)
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Stems | Tunisia |
NP Content: 0.8 %
|
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Stem: (Harvesting time: April)
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Stems | Tunisia |
NP Content: 1.7 %
|
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Stem: (Harvesting time: May)
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Stems | Tunisia |
NP Content: 2.5 %
|
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Stem: (Harvesting time: June)
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Stems | Tunisia |
NP Content: 0.3 %
|
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Stem: (Harvesting time: July)
|
Stems | Tunisia |
NP Content: 0.3 %
|
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Stem: (Harvesting time: August)
|
Stems | Tunisia |
NP Content: 3.3 %
|
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Stem: (Harvesting time: September)
|
Stems | Tunisia |
NP Content: 2.5 %
|
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Stem: (Harvesting time: October)
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Stems | Tunisia |
NP Content: 0.3 %
|
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Stem: (Harvesting time: November)
|
Stems | Tunisia |
NP Content: 0.3 %
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Stem: (Harvesting time: December)
|
Stems | Tunisia |
NP Content: 0.1 %
|
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| Species Name: Ocimum basilicum | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Variety Comparison | [19] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Aerial parts of Ocimum basilicum var. purpurascens Benth, Ocimum basilicum var. dianatnejadii Salimi at flowering stage were collected from plants grown in Experimental Station of Pykan Shahr, near Tehran. Elevation 1215 m above sea level, latitude 35° 42′ North, 51° 8′ East, average humidity 36% and climatic category semi-arid.
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| Factor Function |
Methyl chavicol (43.0%) and linalool (28.9%) were identified as the major compounds in the oil of O. basilicum var. purpurascens, while methyl chavicol (37.6%), linalool (33.4%) and alpha-cadinol (5.7%) were the major constituents in the oil of O. basilicum var. dianatnejadii.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Ocimum basilicum var. dianatnejadii Salimi
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Aerial parts | Iran |
NP Content: 0.3 %
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Ocimum basilicum var. purpurascens Benth
|
Aerial parts | Iran |
NP Content: 0.2 %
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| Factor Name: Drought Stress Treatment | [20] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Seeds of Ocimum basilicum cv. keskenylevelu provided from Hungary, were used in this study. Potted seedlings of Ocimum basilicum were subjected to study the effect of different irrigation rigimes on the essential oil content and composition at experimental farm of college of agriculture, Tarbiat Modarres, University, located in Tehran. (1215 m above sea level, latitude 35° 43′ north, altitude 51° 8′ east). The seeds were sown in spring of 2001 in pots. The irrigation regimes to induce of water stress were: 100%, 85%, 70% and 55% of field capacity. This percentage of field capacity kept constant in the soil by daily weighting of pots. The soil was sandy-loam with 22.6% of field capacity. The harvest of whole plants was performed at the beginning of the flowering stage.
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| Factor Function |
The essential oil content of herb increased from 1.12 to 1.26% as plant water deficit increased (till 70% of field capacity). The number of component of the oil of Ocimum basilicum increased as water stress increase. Amount of the main constituents of the oil such as linalool, methyl chavicol, 1,8-cineole and trans alpha-bergamotene significantly affected by water stress.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
100% Field Irrigation (Control)
|
Whole plant | Mali |
NP Content: 0.4 %
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|
55% Field Irrigation
|
Whole plant | Mali |
NP Content: 0.5 %
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| Species Name: Ocimum basilicum 'Fino Verde' | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Harvest Time Variation; High Temperature Treatment | [21] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
MATERIAL AND METHODS: The study was separated in two experiments performed in our research station Campus Rural of The Federal University of Sergipe (UFS), Sao Cristovao city, Sergipe State, from December 03, 2002 to April 28, 2003. First harvesting: The first harvesting (Experiment 1) was performed 40 days after seedlings transplantation during full bloom on 03/06/2003. Harvesting was performed cutting plants at 20 cm height from the soil. The collected material consisted on separating leaves and inflorescences from the stalk. In the first experiment only used leaves in the analysis. Randomized block design in a 3x4 factorial scheme with three replications was used. Each plot was composed of five plants. Treatments were: three harvesting periods (8:00; 12:00, and 16:00 h) combined with three drying temperatures (40, 50, and 60 ℃) and fresh leaves. Second harvesting: To perform the second harvesting (Experiment 2) we collected the regrowth of plants used in Experiment 1. Plants were harvested fifty three days after the first harvesting (on 04/28/2003) at 8:00 h using the same procedures as the first one; however both leaves and infl orescences were used in the analysis. Randomized block design with three replications was used. Treatments were drying periods of 0, 1, 2, 3, 4, 5, 6, 7, 9, 11, 13, and 16 days for leaves and infl orescences in ovens with air renewal and circulation (Marconi model MA-037/5) at 40 ℃.
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| Factor Function |
Harvesting performed at 8:00 h and 12:00 h provided higher essential oil yield. After five days drying, the concentration of linalool raised from 45.18% to 86.80%. O. basilicum should be harvested during morning and the biomass dried at 40 ℃ for five days to obtain linalool rich essential oil.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Dry Leaf: (Harvesting time: 12:00 h) + (Drying temperature: 50 ℃)
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Leaves | Brazil |
NP Content: 0.7 %
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| Species Name: Pelargonium graveolens L'Her. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Cultivar Comparison; Seasonal Variation | [22] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
One-month-old rooted stem cuttings of three rosescented geranium cultivars, namely Bourbon type, CIM-Pawan and Kelkar were transplanted at 50 × 50 cm plant spacing in the experimental field of Central Institute of Medicinal and Aromatic Plants, Research Center Purara, Uttarakhand, in October 2007. Plants were irrigated immediately after transplanting and further crops were raised following the normal agricultural practices needed to grow the plant. The experimental site is located between the coordinates 28° 60′ and 31° 29′ N, 77° 49′ and 80° 60 m E, and at an altitude of 1250 m in the Kattyur valley. Climatologically, it is categorized as a sub-temperate (1200-1700 m) zone, where monsoon usually breaks in June and continues up to September. Sampling of the rose-scented geranium cultivars was started from March 2008 and taken on the tenth of every month until February 2009. Samples were collected in triplicate in each season. (A, cv. Bourbon type; B, cv. CIM-Pawan; C, cv. Kelkar; I, spring season; II, summer season; III, rainy season;IV, autumn season; V, winter season).
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| Factor Function |
The major components in the essential oil of cv. Bourbon type were geraniol (14.1-34.6%), citronellol (15.2-31.3%), linalool (2.9-9.2%), citronellyl formate (4.4-9.2%), isomenthone (4.5-6.6%), 10-epi-gamma-eudesmol (4.7-6.7%) and geranyl formate (3.8-6.2%). The dominant constituents of the cv. CIM-Pawan essential oil were geraniol (11.9-31.9%), citronellol (16.1-30.2%), citronellyl formate (5.2-8.9%), linalool (3.7-6.4%), isomenthone (4.0-6.3%), 10-epi-gamma-eudesmol (4.4-5.2%) and geranyl formate (4.3-5.0%). However, the chemical composition and odor of cv. Kelkar was quite different from the other two cultivars and the major components found in this oil were citronellol (51.0-63.4%) and isomenthone (9.8-17.8%).
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Pelargonium graveolens cv. Bourbon type: (Harvesting time: spring season)
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Fresh herb | Iran |
NP Content: 0.7 %
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Pelargonium graveolens cv. Bourbon type: (Harvesting time: summer season)
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Fresh herb | Iran |
NP Content: 0.5 %
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Pelargonium graveolens cv. Bourbon type: (Harvesting time: autumn season)
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Fresh herb | Iran |
NP Content: 0.7 %
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Pelargonium graveolens cv. Bourbon type: (Harvesting time: winter season)
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Fresh herb | Iran |
NP Content: 1.2 %
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Pelargonium graveolens cv. Bourbon type: (Harvesting time: rainy season)
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Fresh herb | Iran |
NP Content: 0.8 %
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Pelargonium graveolens cv. CIM-Pawan: (Harvesting time: spring season)
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Fresh herb | Iran |
NP Content: 0.4 %
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Pelargonium graveolens cv. CIM-Pawan: (Harvesting time: summer season)
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Fresh herb | Iran |
NP Content: 0.4 %
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Pelargonium graveolens cv. CIM-Pawan: (Harvesting time: autumn season)
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Fresh herb | Iran |
NP Content: 0.6 %
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Pelargonium graveolens cv. CIM-Pawan: (Harvesting time: winter season)
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Fresh herb | Iran |
NP Content: 1.2 %
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Pelargonium graveolens cv. CIM-Pawan: (Harvesting time: rainy season)
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Fresh herb | Iran |
NP Content: 0.9 %
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| Species Name: Piper nigrum | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Cultivar Comparison; Harvest Time Variation | [23] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
The cultivars selected for this study are Sreekara, Vellanamban and one Indonesian cultivar Kutching grown in Kerala. These cultivars are commonly cultivated in the northern parts of Kerala. The fresh berries of the authenticated cultivars were collected from Indian Institute of Spices Research, Calicut and were dried in a cross flow drier at 45 ℃ and taken for the analysis.
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| Factor Function |
The main components of vellanamban oil were sabinene (3.9-18.8%), beta-pinene (3.9-10.9%), limonene (8.3-19.8%) and beta-caryophyllene (28.4- 32.9%). Sreekara oil contained as major compounds beta-pinene (0-11.2%), limonene (20.1-22.1%) and beta-caryophyllene (16.8-23.1 %). Kutching oil contained alpha-pinene(2.3-5.4%), sabinene (6.7-13.3%), limonene (14.5-17.5%) and beta-caryophyllene (20.8-39.1%).
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Piper nigrum cv. Sreekara: (Harvesting time: 1990)
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Berries | India |
NP Content: 0.1 %
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Piper nigrum cv. Sreekara: (Harvesting time: 1992)
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Berries | India |
NP Content: 0.1 %
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Piper nigrum cv. Vellanamban: (Harvesting time: 1991)
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Berries | India |
NP Content: 0.1 %
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Piper nigrum cv. Vellanamban: (Harvesting time: 1992)
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Berries | India |
NP Content: 0.1 %
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| Species Name: Rosa damascena | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Variety Comparison | [24] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Experimental site: The present study was conducted at the experimental farm of the CSIR-Institute of Himalayan Bioresource Technology, Palampur (1325 m amsl, 32° 06′ 05″ N, 76° 34′10″ E), India, in 2011. Minimum temperature ranges from 3.5 ℃ to 19.8 ℃, maximum temperature ranges from 15.2 ℃ to 31.4 ℃, relative humidity varies between 62.2% and 94.1% in the morning and 45.0% and 87.2% in the evening, and bright sunshine hour ranges from 2.9 to 8.9 hours. Plant material: A population of approximately 50,000 plants raised from mixed stem cuttings collected from perennial rose plantations at the University of Agriculture, Udaipur, Rajasthan, India, and maintained in the field of the CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India, were utilized as an original gene pool of R. damascena. Two varieties, Jwala and Himroz were diversified through selections of desirable traits (morphological/oil content) across 25,000 plants. The five elites, three of R. damascena var. Jwala, (Indica, Super jwala and Jwala) and two of R. damascena var. Himroz (Hot himroz and Himroz) were developed through field selections and maintained at the Natural Plant Products Division Experimental Farm of the Institute. Rosa bourboniana plants were collected from the Fragrance and Flavour Development Centre, Kannauj, UP, India, during 1992 and maintained at the Natural Plant Products Division Experimental Farm of the Institute.
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| Factor Function |
The essential oil content of the varieties of R. damascena varied from 0.037% to 0.051% and that of R. bourboniana was 0.017%. Super jwala recorded the highest oil content (0.051%). A total of 32 components were identified in the different varieties of rose oil. These components constituted 78.1-93.5% of the total rose oil species. The main components of rose oil were citronellol + nerol (16.3-30.1%), geraniol (15.8-29.3%), linalool (0.7-1.9%), rose oxide (0.9-2.6%), phenyl ethyl alcohol (0.1-0.4%), eugenol (0.3-2.2%), nonadecane (7.3-14.7%). The content of citronellol + nerol (30.1%) and geraniol (29.3%) was the highest in Himroz compared with other varieties.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Rosa damascena var. Himroz
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Flowers | India |
NP Content: 1 %
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Rosa damascena var. Hot Himroz
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Flowers | India |
NP Content: 1.7 %
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Rosa damascena var. Indica
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Flowers | India |
NP Content: 1.1 %
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Rosa damascena var. Jwala
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Flowers | India |
NP Content: 2.1 %
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Rosa damascena var. Super Jwala
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Flowers | India |
NP Content: 2.5 %
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| Species Name: Rosamarinus officinalis | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Month Variation; Developmental Stage Variation | [25] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
The plants from Shawieh were harvested four times in 1998 on different separate plants: at full flowering (March), after flowering (May) and at late flowering season (November). And in 1999 at full flowering (March).
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| Factor Function |
The oil samples were found to be rich in alpha-pinene (18.8-38.5%) and 1,8-cineole (19.1-25.1%). The Lebanese oils had particularly high levels of alpha-terpineol (2.9-11.2%) and geraniol (1.8-9.3%). The maximum alpha-pinene content is related to flowering period. Although the results obtained did not indicate a large variation of oil composition in relation to harvest time (flowering and after flowering), some reproducible differences were noticeable.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Whole plant: flowering stage + Harvesting time: March-1998
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Whole plant | Shaweih, Lebanon |
NP Content: 0.3 %
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Whole plant: after flowering stage + Harvesting time: May-1998
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Whole plant | Shaweih, Lebanon |
NP Content: 0.2 %
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Whole plant: late flowering stage + Harvesting time: November-1998
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Whole plant | Shaweih, Lebanon |
NP Content: 0.4 %
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Flower: flowering stage + Harvesting time: March-1999
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Flowers | Shaweih, Lebanon |
NP Content: 0.3 %
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Leaves: flowering stage + Harvesting time: March-1999
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Leaves | Shaweih, Lebanon |
NP Content: 0.3 %
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| Species Name: Rosmarinus eriocalyx | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Locality Variation | [26] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
The leaves of R. eriocalyx were harvested at random from two localities of the forest in the North and South ranges of Boutaleb in Algeria at different altitudes during the full flowering stage. Sample N3(Locality: Northern slope; Altitude (m): 850; Collection date: Mar 20,1993); Sample S3(Locality: Southern slope; Altitude (m): 850; Collection date: Mar 20,1993).
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| Factor Function |
Concerning the alcohols, the highest amount of 1,8-cineole (11.4%) coincided with a very low amount of terpinen-4-ol(1.0%) in sample N3 as well as with a generally low concentration of hydrocarbons (apart from camphene and pinene) in all samples of R. eriocalyx.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Locality: Northern slope, Boutaleb range, Algeria; Altitude 850 m + Harvesting time: 20-Mar-1993
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Leaves | Algeria |
NP Content: 0.1 %
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Locality: Southern slope, Boutaleb range, Algeria; Altitude 850 m + Harvesting time: 20-Mar-1993
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Leaves | Algeria |
NP Content: 0.1 %
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| Species Name: Salvia officinalis | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Altitude Variation; Month Variation | [27] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Sage plant material was collected from two different localities (altitudes 110 and 400 m) in central Herzegovina near Mostar and at four different stages of development: vegetative period (leaves and stalks, January 2003), prior to flowering (leaves and stalks, April 2003), in the course of flowering (flowering tops, leaves and stalks, May 2003) and after flowering (leaves and stalks, August 2003).
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| Factor Function |
The highest oil yield of the plant was after flowering (August). The oil samples obtained prior to flowering (April) and in the course of flowering (May) yielded remarkably less than those after flowering (August) and in the vegetative period (January). An unexpected high oil yield of the plant in the vegetative period (January) is probably due to lower moisture content in this stage of development. The oil yields ranged from 0.29% to 0.64% (altitude 110 m) and 0.45% to 1.07% (altitude 400 m), which reveals that altitude also has significant influence on oil yields. The oils from plant materials gathered prior to flowering (April) and in the course of flowering (May) were found to contain significantly higher percentages of alpha-humulene, manool, viridiflorol and caryophyllene, while the oils produced after flowering (August) and in vegetative period (January) have had higher percentages of alpha-thujone and camphor. Although the altitude has had an obvious influence on oil yields, it did not have significant influence on the qualitative and quantitative composition of their constituents.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Harvesting time: January + Locality: Altitude 110 m
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Whole plant | Bosnia and Herzegovina |
NP Content: 0.3 %
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Harvesting time: August + Locality: Altitude 110 m
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Whole plant | Bosnia and Herzegovina |
NP Content: 0.1 %
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Harvesting time: January + Locality: Altitude 400 m
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Whole plant | Bosnia and Herzegovina |
NP Content: 0.6 %
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| Species Name: Salvia sclarea | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Locality Variation | [28] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
200 g of fresh flowering spikes were collected randomly at full bloom stage (browning of lower floret stage) from the 2006-2007 crops of clary sage cultivar CIM-Chandni cultivated at CIMAP Lucknow and resource center Purara, Uttarakhand. The oil of Kashmir origin was collected from the Chemistry division of IIIM Jammu.
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| Factor Function |
Linalool (23.6%), alpha-terpineol (3.8%), linalyl acetate (51.2%), beta-caryophyllene (3.2%), germacrene D (1.3%) and sclareol (1.3%) were recorded in the oil S. sclarea cultivated in Lucknow UP while the Kashmir oil sample possessed the highest percentage of linalyl acetate (60.8%) and lowest linalool (14.5%) along with alpha-terpineol (1.8%), geranyl acetate (2.2%), beta-caryophyllene (1.9%), germacrene D (2.6%) and sclareol (1.3%) as the other minor constituents. In contrast, the oil of S. sclarea from Purara in Uttarakhand showed highest percentage of linalool (29.8%), alpha-terpineol (5.3%) and sclareol (2.3%) and the lowest linalyl acetate (45.7%) among all the three samples.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Locality: Lucknow UP, India
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Spikes | India |
NP Content: 3.3 %
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Locality: Jammu and Kashmir, India
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Spikes | India |
NP Content: 2.2 %
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Locality: Uttarakhand, India
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Spikes | India |
NP Content: 3 %
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| Factor Name: Altitude Variation | [29] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Clones of T. daenensis populations were collected from 11 locations including seven locations in Fars and four locations in Kohkiluyeh provinces of Iran. The clones of T. daenensis populations were transplanted to the farm at IANRRC Research Station, located in NajafAbad (18 km west Isfahan, 32° 36′ N, 51° 26′ E and 1612 m asl) in March 2002 . Clones were grown in 5 × 2 m plots with 50 × 50 cm planting density. Fertilizers were applied prior to planting at a rate of 60 kg P/ha and 50 kg N/ha. After 3 years (2004), the aerial parts of plants were harvested at full flowering stage, dried at room temperature, and stored until analysis inside paper bags in a cool and dark place. Td1 (Fars Province, Eghlid, Asepas; Altitude: 2000); Td2 (Fars Province, Sourian, Bavanat; Altitude: 2500); Td3 (Fars Province, Abadeh, Keverlar; Altitude: 2280); Td4 (Fars Province, Abadeh -Shiraz Rd, Kolikosh; Altitude: 2400); Td5 (Fars Province, Shiraz -Yasouj Rd, Komehr; Altitude: 2415); Td6 (Fars Province, Yasouj -Shiraz Rd, Margoon; Altitude: 2170); Td7 (Fars Province, Shiraz -Isfahan Rd, Pasargad; Altitude: 2190); Td8 (Kohkiluyeh Province, Sisakht, Gol; Altitude: 2570); Td9 (Kohkiluyeh Province, Kakan; Altitude: 2200); Td10 (Kohkiluyeh Province, Yasouj -Sepidan Rd, Mahparviz; Altitude: 2660); Td11 (Kohkiluyeh Province, Sepidar, Pazanan; Altitude: 2600).
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| Factor Function |
Carvacrol, thymol and geraniol were found as the main constituents in the oils of the tested populations. Variation of the oils in populations was subjected to cluster analysis and three different chemotypes including carvacrol (47.3-80.1%), thymol (53.1-72.2%) and geraniol (65.6-75.7%) were identiified. Other important components were beta-caryophyllene (1.7-9%), p-cymene (0.1-10.9%) and gamma-terpinene (0.1-7.8%). Although Thymus is known as having high thymol content in its oil, it is revealed that T. daenensis subsp. daenensis has also a high potential for carvacrol and geraniol constituents in the oil. The largest similarity of the oil components of populations was detected between Td4 and Td7 and the lowest was revealed between Td8 and Td9. The differences in the oil content and composition of the populations could be attributed to their genetic variability and they could be a good genetic source for breeding purposes.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Locality: Asepas, Eghlid, Fars Province, Iran; Altitude 2000 m
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Aerial parts | Iran |
NP Content: < 0.05 %
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Locality: Bavanat, Sourian, Fars Province, Iran; Altitude 2500 m
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Aerial parts | Iran |
NP Content: 2.7 %
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Locality: Margoon, Yasouj-Shiraz Rd, Fars Province, Iran; Altitude 2170 m
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Aerial parts | Iran |
NP Content: 8.8 %
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Locality: Gol, Sisakht, Kohkiluyeh Province, Iran; Altitude 2570 m
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Aerial parts | Iran |
NP Content: < 0.05 %
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Locality: Kakan, Kohkiluyeh Province, Iran; Altitude 2200 m
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Aerial parts | Iran |
NP Content: 2.1 %
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Locality: Pazanan, Sepidar, Kohkiluyeh Province, Iran; Altitude 2600 m
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Aerial parts | Iran |
NP Content: 9 %
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| Species Name: Satureja thymbra | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Month Variation; Developmental Stage Variation | [30] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Fresh plant materials were obtained in 2004 and 2005. S. thymbra 1(vegetative stage: just before flowering, date: June 7, 2004, location: Mt. Immitos, altitude(m): 350); S. thymbra 2(vegetative stage: full flowering, date: July 7, 2004, location: Mt. Immitos, altitude(m): 350); S. thymbra 3(vegetative stage: after flowering, date: Aug 7, 2004, location: Mt. Immitos, altitude(m): 350); S. thymbra 4(vegetative stage: fruiting, date: Sept 7, 2004, location: Mt. Immitos, altitude(m): 350); S. thymbra 5(vegetative stage: fruiting, date: Nov 7, 2004, location: Mt. Immitos, altitude(m): 350); S. thymbra 6(vegetative stage: fruiting, date: Feb 7, 2005, location: Mt. Immitos, altitude(m): 350); S. thymbra 7(vegetative stage: before flowering, date: May 7, 2005, location: Mt. Immitos, altitude(m): 350); S. parnassica 8(vegetative stage: before flowering, date: June 16, 2004, location: Mt. Parnon, altitude(m): 1800); S. parnassica 9(vegetative stage: just before flowering, date: July 16, 2004, location: Mt. Parnon, altitude(m): 1800); S. parnassica 10(vegetative stage: full flowering, date: Aug 16, 2004, location: Mt. Parnon, altitude(m): 1800); S. parnassica 11(vegetative stage: after flowering, date: Sept 16, 2004, location: Mt. Parnon, altitude(m): 1800).
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| Factor Function |
It is evident that the phytochemical content of the essential oils for both Satureja species varied greatly, depending on the period examined, and showed large prevalence of phenolic content. It must also be pointed out that regardless of the vegetative stage of the plant collected, the sum of the two isomeric phenol monoterpenes (carvacrol and thymol) and their biosynthetic monoterpene precursors p-cymene and gamma-terpinene represented always the bulk of each essential oil (~76%). More specificallysfor both species-during their premature vegetative stage, gamma-terpinene constitutes the major component of their essential oils. The approach of the flowering period results in the simultaneous gradual diminishment of monoterpene precursors and the prevalence of their phenolic metabolites. Thus, essential oils obtained from plants collected during the 'just before their flowering' stage contain thymol as their major component, which constitutes 27.88 and 38.51% of the total oil content for S. thymbra and S. parnassica, respectively. On the other hand, during their full flowering period carvacrol prevails as the major component, accounting for 39.10% for S. thymbra and for 34.61% for S. parnassica. The end of the flowering stage delineates a sharp decrease of carvacrol levels and the predominance of thymol as the major component of the essential oils. A few months later, as the premature vegetative stage approached, the level of gamma-terpinene was restored. The content of p-cymenesthe other major monoterpene precursor-fluctuated seasonally in a manner similar to that shown by gamma-terpinene. Other monoterpene hydrocarbons such as myrcene and alpha-terpinene were also detected in smaller quantities, whereas various monoterpene alcohols such as linalool, borneol, and terpin-4-ol were found mainly in the oils obtained after the flowering stage. Finally, it is notable that the oils obtained during the just before the full flowering period contain beta-caryophyllene as one of their major components.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Harvesting time: just before flowering satge; 7-June-2004
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Leaves and stems | Mt. Immitos, Continental Greece |
NP Content: 0.07 %
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Harvesting time: full flowering satge; 7-June-2004
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Leaves, stems and flowers | Mt. Immitos, Continental Greece |
NP Content: 0.24 %
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Harvesting time: fruiting satge; 7-November-2004
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Leaves and stems | Mt. Immitos, Continental Greece |
NP Content: 0.42 %
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Harvesting time: fruiting satge; 7-February- 2005
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Leaves and stems | Mt. Immitos, Continental Greece |
NP Content: 0.37 %
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Harvesting time: before flowering satge; 7-May-2005
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Leaves and stems | Mt. Immitos, Continental Greece |
NP Content: 0.28 %
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| Species Name: Solidago virgaurea | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Altitude Variation | [31] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Aerial parts of Solidago virgaurea plants were randomly collected from the wild at two different altitudes, as described below, during the 2000 vegetation period. All the collections of the plant samples were carried out during massive bud formation and the beginning of flowering stage. Sample # 1, LTS00-46; 10 kg of the sample was collected on July 31, 2000 at LAT: 51° 07′ LON: 81° 10′ HEI 290 m from Altai land, Lokteev district, near the village of NovoMikhaylovskoe, on the left bank of the Aley River, outskirts of pine forest, fire area, sandy soils. Sample # 2, LTS00-57; 5.6 kg of the sample was collected on August 3, 2000 at LAT 51° 14′ LON 82° 28′ HEI 650 m from Altai land, Kur'in district, around the Kolyvanm quarries, with diverse turf grasses, along the river bank of Aley.
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| Factor Function |
The main components from 290 m were alpha-pinene (36.5%), myrcene (14.8%), beta-caryophyllene (10.5%), germacrene D (8.2%), beta-pinene (7.1%) and limonene+beta-phellandrene (6.4%). The oil from the sample collected at 650 m had benzyl benzoate (57.0%), beta-caryophyllene (6.3%), germacrene D (6.0%), alpha-pinene (4.4%) and alpha-humulene (4.0%) as major components, suggesting polymorphism or the existence of different chemoytpes.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Locality: Lokteev district, Russia; Altitude 290 m
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Flowers | Russia |
NP Content: 0.2 %
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| Species Name: Stachys pilifera | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Locality Variation | [32] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Plant material and isolation procedure: Aerial parts of the plant were collected from two regions, from Kazeroon in southern Iran and Shahr-e-kord in western Iran at the time of flowering in June 2002.
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| Factor Function |
The main components of the oil of S. pilifera collected from Kazeroon, in southern Iran, were spathulenol (15.8%), cis-chrysanthenol (15.3%), beta-caryophyllene (8.4%) and cis-chrysanthenyl acetate (6.9%), while for the plant collected from Shahr-e-kord, in western Iran, they were cis-chrysanthenyl acetate (21.8%), linalool (18.9%), terpinen-4-ol (11.9%) and cis-chrysanthenol (9.2%).
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Locality: Shahr-e-kord, western Iran
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Aerial parts | Iran |
NP Content: 2.6 %
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| Species Name: Tanacetum dolichophyllum | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Altitude Variation | [33] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Wild growing Tanacetum dolichophyllum samples were collected during the period of full flowering, between September-October 2009 from high alpine meadows of Western Himalaya (Uttarakhand, India): Sample I (Dayara, altitude 3200 m) and Sample II (Tungnath, altitude 3800 m).
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| Factor Function |
Plant collected from Dayara meadow (Sample I) afforded cis-lanceol (11.8%), beta-pinene (10.7%), (E)- beta-farnesene (7.4%), alpha-bisabolol (7.2%), beta-eudesmol (5.2%) and terpinen-4-ol (5.1%) as the major constituents, whereas in the sample collected from Tungnath (Sample II) beta-eudesmol (31.4%), alpha-bisabolol (10.7%) were the most abundant components followed by neryl acetate (5.8%) and (E)-beta-farnesene (5.7%). The composition was dominated by sesquiterpene hydrocarbons and oxygen containing sesquiterpenes (49.2-71.1%). The oils are clearly different from those of all other previously reported T. dolichophyllum oils.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Locality: Dayara; Altitude 3200m
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Aerial parts | Himalyas, Uttarakhand, India |
NP Content: <0.05 %
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Locality: Tungnath; Altitude 3800m
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Aerial parts | Himalyas, Uttarakhand, India |
NP Content: 0.2 %
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| Species Name: Thymus longicaulis | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Locality Variation; Developmental Stage Variation | [34] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Fresh plant materials were obtained on 2002. Collection Data: Thymus longicaulis, abbreviation: TLK, vegetative stage: in fruiting, date: 03/06/02, location: Mt. Kitheron, continental Greece, altitude (m): 600; Thymus longicaulis, abbreviation: TLP, vegetative stage: full flowering, date: 17/06/02, location: Mt. Parnon, Peloponnesus, altitude (m): 1650.
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| Factor Function |
T. longicaulis specimens, obtained fromvaried stations, showed large prevalent phenolic contents. The sample of TLK was exceptionally poor in phenolic monoterpenes (35.83%) and the essential oil of OVH was perticularly rich in carvacrol (88.71%).
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Stems, leaves, calyx, and seeds: fruiting stage + (Locality: Mt. Kitheron, continental Greece)
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Stems; Leaves; Calyx; Seeds (fresh) | Mt. Kitheron, continental Greece |
NP Content: 2.98 %
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| Factor Name: Chemotype Comparison | [35] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Aerial parts of the plants with distinct odors, harvested at full flowering stage, were collected from the same population (growing in an area of one m2) on Mt. Parnis Attiki, at an altitude of 1200 m in June 1995.
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| Factor Function |
Limonene (18.7%) and thymol (19.4%); geraniol (56.8%) and geranyl acetate (7.6%); linalool (63.1%) and alpha-terpinyl acetate (20.4%) were the predominant components in each of the three different chemotypes, respectively.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Chemotype (thyme-odor type)
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Aerial parts | Attiki, Greece |
NP Content: 0.1 %
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Chemotype (rose-odor type)
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Aerial parts | Attiki, Greece |
NP Content: 7.6 %
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Chemotype (lavender-odor type)
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Aerial parts | Attiki, Greece |
NP Content: 0.1 %
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| Species Name: Thymus pseudopulegioides | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Locality Variation | [36] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Experiment Detail |
Plant materials were collected from the following localities in north western Turkey. A = Trabzon: Caykara, Soganli dag on July 28, 1994; B = Bayburt: Caykara, Mohakambo yaylasi on July 25, 1994; C = Trabzon: Koprubasi, Vizara yaylasi on July 20, 1994.
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| Factor Function |
One hundred and four compounds were identified representing 97.5-99.5% of the total components detected in thymol/carvacrol (50.14/10.67%), thymol/linalool (23.14/20.24%) and linalool/alpha-terpinyl acetate/geraniol (21.55/16.70/11.17%) rich oils.
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| Factor | Part | Location | NP Content | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Locality: Soganli dag, Caykara, Trabzon, Eskisehir, Turkey
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Aerial parts | Eskisehir, Turkey |
NP Content: 1.4 %
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Locality: Vizara yaylasi, Koprubasi, Trabzon, Eskisehir, Turkey
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Aerial parts | Eskisehir, Turkey |
NP Content: 7.9 %
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| Species Name: Thymus vulgaris | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Factor Name: Cultivar Comparison; Seasonal Variation | [22] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||